Prophylaxis of experimentally induced ovomucoid allergy in neonatal pigs using Lactococcus lactis

Rupa, Prithy; Schmied, Julie; Wilkie, B.N.

doi:10.1016/j.vetimm.2010.11.004

Cited by 21 publications

(18 citation statements)

References 24 publications

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“…To provoke responses, these molecules interact with pattern recognition receptors of the host [83]. A number of reports show that also L. lactis strains can exert similar immune modulatory effects [78,84-86], amongst others driven by teichoic acids [87], also in conjunction with purified allergen [88]. Again, this shows that L. lactis in itself is a good candidate delivery vehicle.…”

Section: Lactis a Top-notch Carrier For Tolerance Inductionmentioning

confidence: 99%

Recombinant Lactococcus lactis can make the difference in antigen-specific immune tolerance induction, the Type 1 Diabetes case

Robert

Steidler

2014

Microb Cell Fact

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Especially in western civilizations, immune diseases that are driven by innocuous (auto- or allo-) antigens are gradually evolving to become pandemic threats. A particularly poignant example is type 1 diabetes, where young children are confronted with the perspective and consequences of total pancreatic β-cell destruction. Along these disquieting observations we find ourselves equipped with impressively accumulating molecular immunological knowledge on the ins and outs of these pathologies. Often, however, it is difficult to translate this wealth into efficacious medicines. The molecular understanding, the concept of oral tolerance induction, the benefit of using recombinant Lactococcus lactis therein and recent openings towards their clinical use may well enable turning all colors to their appropriate fields on this Rubik's cube.

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Section: Lactis a Top-notch Carrier For Tolerance Inductionmentioning

confidence: 99%

Recombinant Lactococcus lactis can make the difference in antigen-specific immune tolerance induction, the Type 1 Diabetes case

Robert

Steidler

2014

Microb Cell Fact

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“…In addition, pigs are outbred, similar to humans, perhaps better mimicking the effect of environmental influences than do inbred mice. The clinical signs of allergy in pigs are similar to those in humans, and several studies have been reported (11,15,16,32,33,35,41).…”

mentioning

confidence: 59%

“…This may have been due to the time of sample collection (96 h); however, previous experiments with cells from pigs of similar age in the same research facility yielded measurable IFN-␥ at this time point (data not shown). Previous research here has shown that the concentrations of IL-4 and IL-10, traditionally considered type 2 cytokines in pigs (29), are elevated in allergic animals and reduced in nonallergic pigs pretreated with live L. lactis (32). Therefore, the elevated concentration of IL-10 in MDP-treated pigs may reflect increased type 2 IR bias, leading to enhanced susceptibility to allergy.…”

Section: Foxp3mentioning

confidence: 72%

“…Immunoglobulin G2 is type 1 associated, while IgG1 is type 2 (8). Pigs pretreated orally with live L. lactis had increased allergen-specific IgG2 AbA levels compared to those of PBS controls, associated with a decrease in sensitization to, and clinical signs of, Ovm allergy (32). Since L. lactis PGN mediates protection against infectious disease in mice and is linked to type 1 induction of IR (28), the decreased activity associated with IgG2 in MDP-treated animals in the present experiment was unexpected.…”

Section: Foxp3mentioning

confidence: 98%

“…of heat-killed Escherichia coli, on each of the first 7 days of life, prevented clinical signs of allergy upon subsequent sensitization and oral challenge with egg white (35). Similarly, live, orally administered Lactococcus lactis treatment prevented allergic sensitization and clinical signs to Ovm and was associated with an increase in type 1 IR bias as indicated by Ig isotype-specific antibody activity (AbA) ratios and a decrease in type 2 cytokine production in treated pigs (32). Although the mechanisms of this reduction in allergy were not investigated, it is likely that interactions between PRRs and microbial-associated molecular patterns were involved.…”

mentioning

confidence: 97%

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Effect of Heat-Killed Escherichia coli, Lipopolysaccharide, and Muramyl Dipeptide Treatments on the Immune Response Phenotype and Allergy in Neonatal Pigs Sensitized to the Egg White Protein Ovomucoid

Schmied

Rupa

Garvie

et al. 2012

Clin Vaccine Immunol

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Predisposition to food allergies may reflect a type 2 immune response (IR) bias in neonates due to the intrauterine environment required to maintain pregnancy. The hygiene hypothesis states that lack of early environmental stimulus leading to inappropriate development and bias in IR may also contribute. Here, the ability of heat-killed Escherichia coli, lipopolysaccharide (LPS), or muramyl dipeptide (MDP) to alter IR bias and subsequent allergic response in neonatal pigs was investigated. Three groups of three litters of pigs (12 pigs/litter) were given intramuscular injections of E. coli, LPS, MDP, or phosphate-buffered saline (PBS) (control) and subsequently sensitized to the egg white allergen ovomucoid using an established protocol. To evaluate change in IR bias, immunoglobulin isotype-associated antibody activity (AbA), concentrations of type 1 and 2 and proinflammatory cytokines released from mitogen-stimulated blood mononuclear cells, and the percentage of T-regulatory cells (T-regs) in blood were measured. Clinical signs of allergy were assessed after oral challenge with egg white. The greatest effect on IR bias was observed in MDP-treated pigs, which had a type 2-biased phenotype by isotype-specific AbA, cytokine production, and a low proportion of T-regs. LPS-treated pigs had decreased type 1-and type 2-associated AbA. E. coli-treated pigs displayed increased response to Ovm as AbA and had more balanced cytokine profiles, as well as the highest proportion of T-regs. Accordingly, pigs treated with MDP were more susceptible to allergy than PBS controls, while pigs treated with LPS were less susceptible. Treatment with E. coli did not significantly alter the frequency of clinical signs.

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Impact of Maternal Selenium Supplementation from Late Gestation and Lactation on Piglet Immune Function

Sun

Ansari

et al. 2019

Biol Trace Elem Res

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Prophylaxis of experimentally induced ovomucoid allergy in neonatal pigs using Lactococcus lactis

Cited by 21 publications

References 24 publications

Recombinant Lactococcus lactis can make the difference in antigen-specific immune tolerance induction, the Type 1 Diabetes case

Recombinant Lactococcus lactis can make the difference in antigen-specific immune tolerance induction, the Type 1 Diabetes case

Effect of Heat-Killed Escherichia coli, Lipopolysaccharide, and Muramyl Dipeptide Treatments on the Immune Response Phenotype and Allergy in Neonatal Pigs Sensitized to the Egg White Protein Ovomucoid

Impact of Maternal Selenium Supplementation from Late Gestation and Lactation on Piglet Immune Function

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