Prophylaxis against Staphylococcus epidermidies vascular graft infection with rifampicin-soaked, gelatin-sealed Dacron

Sardelic, F.; Ao, Peng Y.; Taylor, DJ; Fletcher, J.

doi:10.1016/0967-2109(95)00075-5

Cited by 37 publications

(17 citation statements)

References 18 publications

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“…None of the animals included in the negative control group had anatomic or microbiological evidence of graft infection (no graft contamination). In addition, treatment with these peptides was compared with the conventionally used antibiotic rifampin (63). These experimental groups received grafts presoaked with 5 mg of rifampin per liter alone or rifampin and RIP, DD 13 , or DD 13 -RIP at 10 mg per liter.…”

Section: Resultsmentioning

confidence: 99%

“…Effective strategies for the prevention of prosthetic infections vary and include the use of antimicrobials bound in high concentrations to prosthetic grafts (11,44,63,69,71). Because of the increase in resistance to antibiotics, several studies have focused on developing new prosthetic materials that reduce biofilm formation (28,71).…”

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confidence: 99%

“…These common skin bacteria are also known to be a frequent cause of infections related to prosthetic and indwelling medical devices (16,46), where these infections are related to bacterial biofilm formation on material surfaces, such as venous or urinary catheters, prosthetic heart valves, orthopedic devices, and contact lenses (16,40,46,63). In particular, the late-appearing vascular graft infections are one of the most feared complications that the vascular surgeon treats, frequently resulting in prolonged hospitalization, organ failure, amputation, and death (7, 34).Effective strategies for the prevention of prosthetic infections vary and include the use of antimicrobials bound in high concentrations to prosthetic grafts (11,44,63,69,71). Because of the increase in resistance to antibiotics, several studies have focused on developing new prosthetic materials that reduce biofilm formation (28,71).…”

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confidence: 99%

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A Chimeric Peptide Composed of a Dermaseptin Derivative and an RNA III-Inhibiting Peptide Prevents Graft-Associated Infections by Antibiotic-Resistant Staphylococci

Balaban

Gov

Giacometti

et al. 2004

Antimicrob Agents Chemother

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Staphylococcal bacteria are a prevalent cause of infections associated with foreign bodies and indwelling medical devices. Bacteria are capable of escaping antibiotic treatment through encapsulation into biofilms. RNA III-inhibiting peptide (RIP) is a heptapeptide that inhibits staphylococcal biofilm formation by obstructing quorum-sensing mechanisms. K 4 -S4(1-13) a is a 13-residue dermaseptin derivative (DD 13 ) believed to kill bacteria via membrane disruption. We tested each of these peptides as well as a hybrid construct, DD 13 -RIP, for their ability to inhibit bacterial proliferation and suppress quorum sensing in vitro and for their efficacy in preventing staphylococcal infection in a rat graft infection model with methicillin-resistant Staphylococcus aureus (MRSA) or S. epidermidis (MRSE). In vitro, proliferation assays demonstrated that RIP had no inhibitory effect, while DD 13 -RIP and DD 13 were equally effective, and that the chimeric peptide but not DD 13 was slightly more effective than RIP in inhibiting RNA III synthesis, a regulatory RNA molecule important for staphylococcal pathogenesis. In vivo, the three peptides reduced graft-associated bacterial load in a dosedependent manner, but the hybrid peptide was most potent in totally preventing staphylococcal infections at the lowest dose. In addition, each of the peptides acted synergistically with antibiotics. The data indicate that RIP and DD 13 act in synergy by attacking bacteria simultaneously by two different mechanisms. Such a chimeric peptide may be useful for coating medical devices to prevent drug-resistant staphylococcal infections. Nosocomial infections due to Staphylococcus aureus andStaphylococcus epidermidis have increased over the last decades (36, 41, 42). These common skin bacteria are also known to be a frequent cause of infections related to prosthetic and indwelling medical devices (16,46), where these infections are related to bacterial biofilm formation on material surfaces, such as venous or urinary catheters, prosthetic heart valves, orthopedic devices, and contact lenses (16,40,46,63). In particular, the late-appearing vascular graft infections are one of the most feared complications that the vascular surgeon treats, frequently resulting in prolonged hospitalization, organ failure, amputation, and death (7, 34).Effective strategies for the prevention of prosthetic infections vary and include the use of antimicrobials bound in high concentrations to prosthetic grafts (11,44,63,69,71). Because of the increase in resistance to antibiotics, several studies have focused on developing new prosthetic materials that reduce biofilm formation (28,71). One of the most interesting studies was based on the fact that the resistance of a biofilm to antimicrobial agents is acquired as a multicellular strategy that relies on exchange of chemical signals between cells in a process known as quorum sensing (48). Thus, interfering with this mechanism of bacterial cell-cell communication could provide a novel approach to prevent biofilm forma...

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A Chimeric Peptide Composed of a Dermaseptin Derivative and an RNA III-Inhibiting Peptide Prevents Graft-Associated Infections by Antibiotic-Resistant Staphylococci

Balaban

Gov

Giacometti

et al. 2004

Antimicrob Agents Chemother

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“…In the case of vascular grafts, antimicrobials such as rifampin, bounded in high concentrations to prosthetic grafts, have been proposed as adjunctive prophylaxis. 17,18 Today, novel associations of promising molecules with antimicrobial capacity are being tested. 19 A novel way to prevent biofilm formation would be to interfere with bacterial cell-cell communication that leads to the virulence phenotype.…”

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confidence: 99%

Prophylactic Efficacy of Topical Temporin A and RNAIII-Inhibiting Peptide in a Subcutaneous Rat Pouch Model of Graft Infection Attributable to Staphylococci With Intermediate Resistance to Glycopeptides

et al. 2003

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Background-Bacteria that adhere to implanted medical devices play an important role in industry and in modern medicine. Staphylococci are among the most common pathogens that cause biomaterial infections. Vascular prosthetic graft infection is one of the most feared complications that the vascular surgeon treats, frequently resulting in prolonged hospitalization, organ failure, amputation, and death. A rat model was used to investigate the topical efficacies of temporin A and the quorum-sensing inhibitor RNAIII-inhibiting protein (RIP) as prophylactic agents of vascular prosthetic graft infections caused by Staphylococcus aureus and Staphylococcus epidermidis with intermediate resistance to glycopeptides. Methods and Results-Graft infections were established in the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses 1 cm 2 followed by topical inoculation with 2ϫ10 7 colony-forming units of bacterial strains. The study included, for each staphylococcal strain, a control group (no graft contamination), a contaminated group that did not receive antibiotic prophylaxis, and 6 contaminated groups that received grafts soaked with temporin A, RIP, rifampin, temporin A plus RIP, RIP plus rifampin, or temporin A plus RIP. The infection was evaluated by quantitative agar culture. When tested alone, temporin A and RIP showed comparable efficacies, and their efficacies were significantly higher than that of rifampin against both strains. All combinations showed efficacies significantly higher than that of each single compound. The combinations of temporin A and RIP exerted the strongest antistaphylococcal efficacies, eliminating infection by 100%. Conclusions-The

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Prophylaxis against Staphylococcus aureus Vascular Graft Infection with Mupirocin-Soaked, Collagen-Sealed Dacron

Ghiselli

Giacometti

Goffi

et al. 2001

Journal of Surgical Research

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Prophylaxis against Staphylococcus epidermidies vascular graft infection with rifampicin-soaked, gelatin-sealed Dacron

Cited by 37 publications

References 18 publications

A Chimeric Peptide Composed of a Dermaseptin Derivative and an RNA III-Inhibiting Peptide Prevents Graft-Associated Infections by Antibiotic-Resistant Staphylococci

A Chimeric Peptide Composed of a Dermaseptin Derivative and an RNA III-Inhibiting Peptide Prevents Graft-Associated Infections by Antibiotic-Resistant Staphylococci

Prophylactic Efficacy of Topical Temporin A and RNAIII-Inhibiting Peptide in a Subcutaneous Rat Pouch Model of Graft Infection Attributable to Staphylococci With Intermediate Resistance to Glycopeptides

Prophylaxis against Staphylococcus aureus Vascular Graft Infection with Mupirocin-Soaked, Collagen-Sealed Dacron

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