Prophylactic, therapeutic and anti-metastatic effects of BMDC and DC lines in mice carrying HPV 16-associated tumours

Mendoza, Luis; Bubenı́k, Jan; Šímová, Jana; Jandlová, T; Vonka, V.; Mikyšková, Romana

doi:10.3892/ijo.23.1.243

Cited by 10 publications

(13 citation statements)

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“…These cells, due to their homogeneous and well-characterized features, are thought to be technically easier to prepare than fresh BM-DCs. It was found that JAWS II cells, applied as an antitumor vaccine, effectively induced a protective and therapeutic antitumor response against HPV 16-associated tumors (Mendoza et al 2002;Mendoza et al 2003). The cells are also useful for optimization of DC transduction conditions.…”

Section: Discussionmentioning

confidence: 97%

Generation of antitumor response by IL-2-transduced JAWS II dendritic cells

et al. 2011

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Section: Discussionmentioning

confidence: 97%

Generation of antitumor response by IL-2-transduced JAWS II dendritic cells

et al. 2011

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“…Here, we characterized an immature DC line, JAWS II, which originated from BMDCs of p53 Ϫ/Ϫ C57BL/6 mice. JAWS II cells have already been used for study of other infectious pathogens (23,38) and compared with BMDCs (15,22). However, they have not yet been studied for their interaction with Chlamydia.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, for immunoproteomic experiments large numbers of mice have to be sacrificed to generate enough primary BMDCs in order to purify sufficient antigen-loaded major histocompatibility complexes (MHCs) for mass spectrometry. These limitations could be overcome with an immortal DC line if it should have stable characteristics and be able to grow at high density, thus satisfying quantitative requirements and providing standardized quality (8,15,22).…”

mentioning

confidence: 99%

Characterization of Murine Dendritic Cell Line JAWS II and Primary Bone Marrow-Derived Dendritic Cells inChlamydia muridarumAntigen Presentation and Induction of Protective Immunity

et al. 2008

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“…DC, being the primary antigen capturing and presenting cell of the immune system, offers an excellent experimental system to understand the adjuvant effects of LPD. The murine cell line DC2.4, which has proven to be a good in vitro model for the antigen presenting cells (APCs) was selected for these studies [13,14]. The observations were further correlated with primary dendritic cells derived from the murine bone marrow.…”

Section: Introductionmentioning

confidence: 99%

Immunostimulation of dendritic cells by cationic liposomes

Dileep¹,

Cui²,

Chen³

et al. 2006

Molecular Membrane Biology

153

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A nano-aggregate liposome-polycation-DNA (LPD), composed of a cationic lipid, protamine and plasmid DNA was found to effectively deliver a human papillomavirus (HPV)-E7 epitope antigen to the antigen presenting cells of the immune system, eliciting enhanced anti-tumor immune responses in mouse models of cervical carcinoma. Both the cationic liposome and plasmid DNA were essential for the full immunostimulation activity of LPD. Interestingly, cationic liposomes alone could stimulate the antigen presenting dendritic cells (DC) leading to the expression of co-stimulatory molecules, CD80 and CD86. However, cationic lipids could not stimulate DC for the expression of pro-inflammatory cytokines. Moreover, they were unable to enhance the expression of NF-kappaB, suggesting that dendritic cells stimulation by cationic lipids is signaled through an NF-kappaB independent mechanism. DC stimulation was specific to cationic lipids, the zwitterionic and anionic lipids showed little or no activity. The ability of different cationic lipids to stimulate the expression of co-stimulatory molecules on DC varied significantly. In general, the cationic lipids bearing ethyl phosphocholine head groups were better stimulants than their trimethylammonium counterparts. In case of the cationic lipids bearing trimethyl ammonium head groups, the ones bearing unsaturated or shorter saturated hydrophobic chains exhibited enhanced immunostimulatory activity. The LPS-induced TNF-alpha expression by dendritic cells was inhibited by active cationic lipids but not the inactive ones, suggesting the possible involvement of lipopolysaccharide binding protein (LBP) in cationic lipid mediated DC stimulation. Based on the structure-specific activation of dendritic cells by cationic lipids, a model for the immunostimulation of DC by such lipids is proposed.

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Prophylactic, therapeutic and anti-metastatic effects of BMDC and DC lines in mice carrying HPV 16-associated tumours

Cited by 10 publications

References 0 publications

Generation of antitumor response by IL-2-transduced JAWS II dendritic cells

Generation of antitumor response by IL-2-transduced JAWS II dendritic cells

Characterization of Murine Dendritic Cell Line JAWS II and Primary Bone Marrow-Derived Dendritic Cells inChlamydia muridarumAntigen Presentation and Induction of Protective Immunity

Immunostimulation of dendritic cells by cationic liposomes

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