Properties of transposon SCTn1 of Streptomyces coelicolor A3(2)

Sermonti, G.; Lanfaloni, Luisa; Micheli, Maria Rita

doi:10.1007/bf00330904

Cited by 7 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transposons have been suggested to be involved in the alterations (SERMONTI et al 1983, NAKANO et al 1984. For about ten years plasmids are known to possibly take part in the control of secondary metabolism.…”

mentioning

confidence: 98%

“…Several authors were able to show correlations between the altered phenotype and structural changes in the chromosomal DNA (HINTERMA" et al 1981, ONO et al 1982, CRAMKRI et al 1983, FISHMAN and HERSHBERGER 1983, ALTENBUCHNER and CULLUM 1984. Transposons have been suggested to be involved in the alterations (SERMONTI et al 1983, NAKANO et al 1984. For about ten years plasmids are known to possibly take part in the control of secondary metabolism.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Directed selection of differentiation mutants of Streptomyces noursei using chemostat cultivation

Noack¹

1986

J. Basic Microbiol.

View full text Add to dashboard Cite

A nourseothricin-producing Streptomyces noursei strain was continuously cultivated in a chemostat equipped with a stirrer for mechanical fractionation of the mycelium. Different cultivation conditions allowed the selection of six types of differentiation mutants after the culture had reached a population genetically stationary state. The mutants showed an altered control pattern of sporulation as well as altered antibiotic biosynthesis and antibiotic resistance. In addition, the stability of the recombinant plasmid pIJ385 in several differentiation type mutants as host strains was tested. The results suggest that there exists a strong correlation between the cultivation conditions employed and the type of differentiation mutants selected.

show abstract

mentioning

confidence: 98%

mentioning

confidence: 99%

Directed selection of differentiation mutants of Streptomyces noursei using chemostat cultivation

Noack¹

1986

J. Basic Microbiol.

View full text Add to dashboard Cite

show abstract

“…On the other hand, SERMONTI et al 15) predicted that S. coelicolor might contain a "jumping" gene capable of transposing onto plasmid SCPI. In spite of these findings, there remains much to be studied about the biological and physical properties of streptomycete plasmids.…”

mentioning

confidence: 99%

Mutual relation of three pock-forming plasmids resident in Streptomyces noursei.

1985

View full text Add to dashboard Cite

From Streptonryces noursei B3, two plasmids -designated pSCY5 and pSCY6 -were isolated in addition to the known plasmid pSCY3. Strains carrying one or more of these plasmids generated pocks on a lawn of the plasmid-free strain, S. noursei KL3. The pocks elicited by pSCY3 and pSCY5 belonged to the A type; i.e., showed a clear inhibition zone, while those produced by pSCY6 were of the B type; i.e., exhibited a turbid inhibition zone. The strains carrying either pSCY3 or pSCY5, were also capable of forming pocks on a lawn of S. noursei 6T-11 harboring pSCY6, and vice versa. However, pock formation was not observed between strains harboring pSCY3 and strains carrying pSCY5. The endonuclease cleavage maps of these plasmids revealed that pSCY3 differed clearly from that of pSCY6, whereas pSCY5 was found to be a hybrid plasmid consisting of the entire pSCY3 plasmid and an 8.4 Md or longer fragment originating from pSCY6. The pocks elicited by pSCY5 were much smaller than those produced by pSCY3. Transformation experiments showed that pSCY6 elicited pocks in Streptomyces lividans as well as in S. noursei, whereas the pSCY6 transformants of S. lividans failed to produce pocks on a lawn of plasmid-free S. noursei.Numerous plasmids have recently been isolated from streptomycetes. Their functions have been examined from the point of view of antibiotic biosynthesis, regulation of antibiotic production, resistance to antibiotics by the producing organisms, relation with pleiotropic phenotypes, etc.1~5). However, their role is still poorly understood, except in the case of the plasmid carrying the genetic determinants for methylenomycin biosynthesis and resistance, sex plasmids and self-transmissible plasmids capable of producing pocks5~9). Pock formation, initially designated as lethal zygosis, was described by BIBB et al.7) in the case of SCP2 and SCP2* plasmids of Streptomyces coelicolor A3 (2)8) and this phenomenon is thought to be characteristic of several streptomycete plasmids10~12) Subsequently, KIESER et al.13) assigned the probable pock-determinant region to a part of piJ101 which was named the "spread" region . In addition to the detailed biological studies of plasmids, BIBB et al.14) showed that SLP1 plasmids in Streptonryces lividans were formed by excision of DNA segments from the chromosome of S. coelicolor during mating.On the other hand, SERMONTI et al.15) predicted that S. coelicolor might contain a "jumping" gene capable of transposing onto plasmid SCPI. In spite of these findings, there remains much to be studied about the biological and physical properties of streptomycete plasmids.S. noursei 2217-G 1 is a producer of cycloheximide and nystatin, and carries several types of plasmids.HAYAKAWA et al.16) reported the isolation of plasmids pSCY1 and pSCY2 from this streptomycete, but their functions were not elucidated. TAKEDA et al.11) of our laboratory, described pSCY2, pSCY3 and pSCY4 in S. noursei variant strain B3 and constructed the endonuclease cleavage map of pSCY3 which had been transformed into Esche...

show abstract

Evidence of heterokaryosis in Streptomyces coelicolor A3(2)

1984

View full text Add to dashboard Cite

Hyphae from mixed cultures of complementary auxotrophs of Streptomyces coelicolor A3(2) did not grow on minimal media (MM) when fertility plasmids ( SCP1 and SCP2) were missing in both strains. The addition of one part per cent of complete medium (CM) to MM allowed growth of vigorous colonies among the tiny colonies of the parental types. The former, amounting to 1%-10% of the total population, turned out to be heterokaryons. They could be propagated on the same medium by plating of hyphal fragments. When five parts per cent of CM were added to MM, beside the heterokaryotic colonies, vigorous 'spindles' of aerial mycelium were formed whenever complementary colonies overlapped. When the SCP1 and SCP2 plasmids were present in one or both parents a clear constraint on the outburst of heterokaryotic aerial mycelium was observed.

show abstract

Properties of transposon SCTn1 of Streptomyces coelicolor A3(2)

Cited by 7 publications

References 15 publications

Directed selection of differentiation mutants of Streptomyces noursei using chemostat cultivation

Directed selection of differentiation mutants of Streptomyces noursei using chemostat cultivation

Mutual relation of three pock-forming plasmids resident in Streptomyces noursei.

Evidence of heterokaryosis in Streptomyces coelicolor A3(2)

Contact Info

Product

Resources

About