Properties of the Probiotic Strain Lactobacillus plantarum 8-RA-3 Grown in a Biofilm by Solid Substrate Cultivation Method

Abstract

The biofilm formation took place in 48 h within the solid substrate cultivation of Lactobacillus plantarum 8-RA-3 strain on the wheat bran saturated with the MRS medium. The drying of the bran fermented by lactobacilli resulted in a decrease in the number of colony-forming units (CFU) from 23.0 × 10(8) to 6.9 × 10(5) CFU/g in daily samples and to less than 10(4) CFU/g in 2- and 3-day samples. However, according to the fluorescence-based live/dead assay data, more than 40 % of the non-cultured bacteria were viable. As a result of mice kept on a diet with the introduction of bran fermented by Lact. plantarum 8-RA-3 for 72 h into the fodder, a recovery of normal level of intestinal lactobacilli, inhibited by administration of antibiotic was noted. The strain genetically identical to the Lact. plantarum 8-RA-3 was isolated from the feces of these mice. The results indicate that solid substrate cultivated Lact. plantarum 8-RA-3 strain formed a biofilm. Once dried and transferred into a non-cultured state, biofilm cells retained its viability and biological activity.

Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation

“…F613461, Sangon Biotech Co., Ltd., Shanghai, China) to filter the GM group culture, and then washed the particles to wash away planktonic cells. The colony-forming units (CFU) and pH values in the GM and control culture group were determined at 16, 34, 68, and 86 h during fermentation [31]. For GM culture group CFU counting, samples were vortexed for 30 s, sonicated for 10 s (Vibra Cell Model VCX150PB, Sonics & Materials Inc., Danbury, CT, USA) and vortexed again for 30 s in order to disperse biofilm cells into the suspension [32].…”

Poultry-beneficial solid-state Bacillus amyloliquefaciens B-1895 fermented soybean formulation

“…Ushakova et al [ 10 ] indicated the importance of biofilms and their promising application in medical and veterinary probiotic formulations, specifically those generated by B. subtilis and other spore-forming probiotic microorganisms [ 11 ]. These produce nutritionally-functional products capable of strong antagonistic activity to pathogens [ 12 ], technological plasticity and high enzymatic activity [ 13 ].…”

A Review of the Effects and Production of Spore-Forming Probiotics for Poultry

“…However, this phenomenon has a second side. Probiotic bacteria combined in biofilms grow better, are more resistant to drying, and can colonize the gastrointestinal tract of the host more efficiently [221].…”