1978
DOI: 10.1073/pnas.75.8.3693
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Properties of the interaction of fluoride- and guanylyl-5'-imidodiphosphate-regulatory proteins with adenylate cyclase.

Abstract: The mechanism of activation of adenylate cyclase by guanylyl-5'-imidodiphosphate [Gpp(NH)p] and NaF has been investigated by studying the reconstitution of Gpp(NH)p and NaF sensitivity of an enzyme rendered insensitive to these agents by differential detergent extraction of a particulate brain enzyme. Such reconstitution can be achieved by the addition of macromolecular regulatory factors from membranes of various tissues. Trypsin digestion and thermal inactivation provide evidence for the existence of two dis… Show more

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Cited by 26 publications
(17 citation statements)
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“…The largest molecular form derived from Triton shells may include one or more cytoskeletal elements. The intermediate molecular entity appears to be similar in size to that reported earlier (21) and purified subsequently (15 (17,28). The recent determination of the subunit structure of the N-protein (15) should facilitate further elucidation of these molecular changes.…”
Section: Discussionsupporting
confidence: 67%
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“…The largest molecular form derived from Triton shells may include one or more cytoskeletal elements. The intermediate molecular entity appears to be similar in size to that reported earlier (21) and purified subsequently (15 (17,28). The recent determination of the subunit structure of the N-protein (15) should facilitate further elucidation of these molecular changes.…”
Section: Discussionsupporting
confidence: 67%
“…In addition, we also demonstrated a reversible interaction between this protein and p[NH]ppG, leading to a conformational change in the N-protein which altered its sensitivity to proteolysis and thermal inactivation (17). This activated N-protein formed a highaffinity complex with a catalytic component, resulting in the activation of adenylate cyclase (17). Affinity chromatography (18) and somatic cell variants (19) have also been used to isolate and characterize the N-protein.…”
Section: Discussionmentioning
confidence: 99%
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