Properties of the chitinase of the antifungal biocontrol agent Streptomyces lydicus WYEC108

Mahadevan, B; Crawford, Don L.

doi:10.1016/s0141-0229(96)00175-5

Cited by 152 publications

(83 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is produced commercially to control soil-borne and leaf surface diseases in turf and greenhouse crops (27). WYEC108 produces chitinase (14), which functions synergistically with several antibiotics (9,23) to kill other fungi.…”

Section: Discussionmentioning

confidence: 99%

Identification of an antifungal metabolite produced by a potential biocontrol Actinomyces strain A01

Liu²,

Qiu³

et al. 2008

Braz. J. Microbiol.

View full text Add to dashboard Cite

Actinomyces strain A01 was isolated from soil of a vegetable field in the suburb of Beijing, China. According to the morphological, cultural, physiological and biochemical characteristics, and 16S rDNA sequence analysis, strain A01 was identified as Streptomyces lydicus. In the antimicrobial spectrum test strain A01 presented a stable and strong inhibitory activity against several plant pathogenic fungi such as Fusarium oxysporum, Botrytis cinerea, Monilinia laxa, etc. However, no antibacterial activity was found. In pot experiments in greenhouse, the development of tomato gray mold was markedly suppressed by treatment with the fermentation broth of the strain A01, and the control efficacy was higher than those of Pyrimethanil and Polyoxin. A main antifungal compound (purity 99.503%) was obtained from the fermentation broth of strain A01 using column chromatography and HPLC. The chemical structural analysis with UV, IR, MS, and NMR confirmed that the compound produced by the strain A01 is natamycin, a polyene antibiotic produced by S. chattanovgensis, S. natalensis, and S. gilvosporeus, widely used as a natural biological preservative for food according to previous reports. The present study revealed a new producing strain of natamycin and its potential application as a biological control agent for fungal plant diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of an antifungal metabolite produced by a potential biocontrol Actinomyces strain A01

Liu²,

Qiu³

et al. 2008

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Streptomyces promote plant growth either by producing indole-3-acetic acid (Aldesuquy et al 1998) or siderophores (Tokala et al 2002). Streptomyces has also been extensively studied and used for biocontrol of soil-borne fungal pathogens (Mahadevan and Crawford 1997;Trejo-Estrada et al 1998;Macagnan et al 2008.…”

Section: Introductionmentioning

confidence: 99%

Evaluation ofStreptomycesspp. for their plant-growth-promotion traits in rice

et al. 2013

View full text Add to dashboard Cite

Five strains of Streptomyces were earlier reported to have potential for charcoal rot control and plant growth promotion (PGP) in sorghum. In this study, those five Streptomyces strains were characterized for their enzymatic activities and evaluated for their PGP capabilities on rice. All the Streptomyces were able to produce lipase, β-1,3-glucanase, grew in NaCl (up to 8%), at pH 5−13, temperatures 20−40 o C and were resistant to ampicillin, sensitive to nalidixic acid and highly sensitive to chloramphenicol, kanamycin, streptomycin and tetracycline. They were highly tolerant to fungicide bavistin, whereas highly sensitive to benlate, benomyl and radonil. When evaluated on rice in the field, the Streptomyces significantly enhanced tillers, panicles, stover yield, grain yield, dry matter, root length, volume and dry weight over the control. In the rhizosphere at harvest, microbial biomass carbon and nitrogen, dehydrogenase activity, total N, available P and % organic carbon were also found significantly higher in Streptomyces treated plots over the control. This study further confirms that the selected Streptomyces have PGP activities.

show abstract

“…In this instance the chitin serves as a source of energy and nutrients (principally C and N) to a microbial culture that is capable of producing one or more useful products. Mahadevan and Crawford (1997) cultured Streptomyces lydicus on chitin to produce chitinases with anti-fungal activity, whilst Trejo-Estrada et al, (2000) found that S.…”

Section: Introductionmentioning

confidence: 99%

Production of streptomycin from chitin using Streptomyces griseus in bioreactors of different configuration

Meanwell

Shama

2008

Bioresource Technology

View full text Add to dashboard Cite

• This is a journal article. Rushton impellers and operated at high speed was used, the yield of streptomycin (3.1 mg/l) was the highest observed and occurred at approximately 500 hours. Cultivation of S. griseus in a bioreactor stirred at low speed by a U-shaped paddle resulted in a lower yield of streptomycin (1.8 mg/l) but this was achieved in a shorter period of time (400 hours). Increasing the concentration of chitin from 5 to 10 % w/v had no significant effect on either of these two parameters. The use of a novel vertical basket bioreactor in which the chitin flakes were contained within a wire mesh basket and were gently fluidised by air, enabled comparatively high yields of streptomycin (2.8 mg/l) in the relatively short time of 300 hours.

show abstract

Properties of the chitinase of the antifungal biocontrol agent Streptomyces lydicus WYEC108

Cited by 152 publications

References 22 publications

Identification of an antifungal metabolite produced by a potential biocontrol Actinomyces strain A01

Identification of an antifungal metabolite produced by a potential biocontrol Actinomyces strain A01

Evaluation ofStreptomycesspp. for their plant-growth-promotion traits in rice

Production of streptomycin from chitin using Streptomyces griseus in bioreactors of different configuration

Contact Info

Product

Resources

About