Properties of some citrus seeds. Part 1. Physico-chemical characteristics of proteins

Abstract

The protein solubility of some citrus seeds flours as well as physico‐chemical characteristics were determined by gel filtration, polyacrylamide gel electrophoresis (PAGE), polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate (SDS‐PAGE), and UV‐spectrum. The highest solubility was in 0.1 M NaOH followed by both 0.1 M KCl and NaCl, respectively. The lowest solubility was in water. The protein solubility showed a minium with shift towards the acidic side in presence of 0.1 M and 0.5 M NaCl. The PAGE pattern showed two major protein fractions for citron, orange and mandarin seeds. SDS‐PAGE proved presence of 3 major polypeptide chains in all seeds proteins. The apparent molecular weight was 37 304, 23 516 and 16 740 daltons, respectively. Also, the major protein fraction in all samples was globulin. The polypeptide chains were 4 for both albumins and globulins, 3 and 2 for prolamins and glutelins, respectively. Gel filtration revealed four peaks in all samples. The major peaks were eluted at 79 and 160 ml. All peaks were principally free from nucleic acids except peak No. 4.

Lipid Evaluation of Some Tunisian Citrus Seeds

“…The chemical composition and mineral content of Citrus seeds, the characteristics and structure specifics of their oils as well as their nutritional properties have been studied. According to El‐Adawy et al. (1999), citrus seeds are rich in oil and protein and also serve as a good source of K, Ca, Na, Fe and Mg.…”

Changes in Lipid Composition and Antioxidant Capacity of Bitter Orange (Citrus aurantium. L) and Mandarin (Citrus reticulata. Blanco) Oilseeds on Different Stages of Maturity

“…However, the importance of Citrus fruit is not only due to its essential oils but also because of the importance of its seeds that contain different components that can be used for value-added production of a number of products. According to El-Adawy et al [1], Citrus seeds are rich in oil and protein and also serve as a good source of K, Ca, Na, Fe and Mg. They are also rich in limonoids and their glucoside derivative [2].…”