Properties of scaffolds based on chitosan and collagen with bioglass 45S5

Kaczmarek, Beata; Nadolna, K.; Owczarek, A.; Mazur, Olha; Sionkowska, Alina; Łukowicz, Krzysztof; Vishnu, Jithin; Manivasagam, Geetha; Osyczka, Anna M.

doi:10.1049/iet-nbt.2020.0045

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…Furthermore, characteristic peaks for collagen (Amide I, II, and III), as well as a typical peak corresponding to Amide A, hydroxyl, and amine groups, were observed. This is in line with our previous study, where we confirmed that the interaction between collagen and chitosan hydrogen occurs without new covalent formation [62]; the addition of melatonin did not influence its polymeric structure. It should, however, be noted that an increased number of hydroxyl groups in the resulting biomaterials determines elevated water binding.…”

Section: Discussionsupporting

confidence: 93%

“…Namely, collagen can be blended with other proteins, such as silk fibroin or elastin [ 57 , 58 ], but also with polysaccharides, including hyaluronic acid [ 59 ], chitosan [ 60 ], or sodium alginate [ 61 ]. Following an earlier study of Kaczmarek et al [ 62 ], herein, we investigated different scaffolds based on collagen derived from rat tail tendon or from Salmo salar fish skin mixed with chitosan. Resultant matrices were assessed as carriers for melatonin.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing

Kaczmarek-Szczepańska

Ostrowska

Kozłowska

et al. 2021

IJMS

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The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms of its potential clinical importance for future therapies of cutaneous diseases. Mammalian skin is not only a prominent melatonin target, but also produces and rapidly metabolizes the multifunctional methoxyindole to biologically active metabolites. In our methodology, chitosan/collagen (CTS/Coll)-contained biomaterials are blended with melatonin at different doses to fabricate biomimetic hybrid scaffolds. We use rat tail tendon- and Salmo salar fish skin-derived collagens to assess biophysical and cellular properties by (i) Fourier transform infrared spectroscopy—attenuated total reflectance (FTIR–ATR), (ii) thermogravimetric analysis (TG), (iii) scanning electron microscope (SEM), and (iv) proliferation ratio of cutaneous cells in vitro. Our results indicate that melatonin itself does not negatively affect biophysical properties of melatonin-immobilized hybrid scaffolds, but it induces a pronounced elevation of cell viability within human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF), and reference melanoma cells. These results demonstrate that this indoleamine accelerates re-epithelialization. This delivery is a promising technique for additional explorations in future dermatotherapy and protective skin medicine.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing

Kaczmarek-Szczepańska

Ostrowska

Kozłowska

et al. 2021

IJMS

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“…Despite the advantages of collagen and chitosan scaffolds in the tissue engineering the main drawback is their biological inertness aspect. Accordingly, bioactive fillers (such as, bioactive glass) have been suggested to be incorporated in this kind of polymers to improve their bioactivity (Kaczmarek et al, 2020;Gao et al, 2021;Deen et al, 2022). Moreover, the scaffold can be functionalized with by doping of bioactive glass filler with therapeutic ions such as, silver, copper and cerium, in these fillers.…”

Section: Advances Inmentioning

confidence: 99%

Ce-doped Nanobioactive Glass/ Collagen/ Chitosan Composite Scaffolds: Biocompatibility with Normal Rabbit’s Osteoblast Cells and Anticancer Activity Test

Hammouda¹,

Farag²,

Deftar³

et al. 2022

Adv. Anim. Vet. Sci.

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This research aims to evaluate cerium-doped nanobioactive glass/collagen/chitosan composites scaffolds with osteoblast mineralization of normal rabbit bone marrow mesenchymal stem cells (rBM-MSCs) and cancer osteosarcoma cells. The non-cellular in vitro bioactivity test was performed in simulated body fluids for periods 1, 3, 10, 20 and 30 d by measuring the calcium and phosphate ion concentrations by SEM/EDX analysis. While, the bioactivity of expanded and differentiated osteoblast cells derived from isolated rBM-MSCs by flowcytometric analysis was studied by histochemical staining with Alizarin Red and von Kossa to confirm the osteogenic differentiation process. Also, cell viability assay by MTT was used to measure the number of viable osteoblast cells cultured with scaffolds extracts. Also, the antitumor activity of the scaffolds was studied against cancer osteosarcoma cell lines using Sulforhodamine B (SRB) assay. The results showed that addition of cerium-doped nanobioactive glass to the composite scaffolds was triggered an increase in cell growth, proliferation and mineralization markers of osteoblast cells that increased with time as the highest concentrations of CeO 2 in nanobioactive glass (sample CL/CH/C10). Cell viability proved also that all scaffolds and their extracts showed proliferation inhibition with time < 25% reference to final cell number of control cells. Among the composites, having CL/CH/C5 showed the highest cytotoxic effect and reduced survival rate of osteosarcoma cells to 75.68% after 24 h. The subsequent increase of CeO 2 concentration was also effective but its effect was less than CL/CH/C5 sample. Finally, cerium-doped nanobioactive glass/collagen/chitosan composites scaffolds were exhibited good biocompatibility on normal cells and increased cytotoxicity on cancer osteosaroma cells.

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