Properties of protein isolates prepared from ground seeds

Abstract

A dye binding procedure has been developed for the estimation of protein in seed extracts and solutions of seed protein isolates. The method was evaluated using extracts of five seed materials, viz. field beans (Phaseolus vulgaris), rapeseed (Brassica napus) , sesame seed (Sesame indicum), cowpeas ( Vigna unguiculata) and cottonseed (Gossypiurn hirsutum L.). A high correlation between the dye binding measurements and analyses obtained by the Kjeldahl procedure has been demonstrated and the suitability of the dye binding method for routine protein estimations in this work has been confirmed.