Properties of probiotic bacteria explored by proteomic approaches

Guchte, Maarten van de; Chaze, Thibault; Jan, Gwénaël; Mistou, Michel‐Yves

doi:10.1016/j.mib.2012.04.003

Cited by 17 publications

(9 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Proteomic-based probiotic research is beginning to inform both researchers and industry that adaptation and adherence properties specific to probiotic strains influence their ability to colonize the host (Siciliano and Mazzeo, 2012; van de Guchte et al, 2012). Additionally, these adaptation and adherence mechanisms have been reported to potentially be strain specific, making it difficult to globally apply these mechanisms to all probiotic bacterial strains (Siciliano and Mazzeo, 2012).…”

Section: The Aging Gutmentioning

confidence: 99%

Diet and the development of the human intestinal microbiome

2014

View full text Add to dashboard Cite

The important role of the gut microbiome in maintaining human health has necessitated a better understanding of the temporal dynamics of intestinal microbial communities as well as the host and environmental factors driving these dynamics. Genetics, mode of birth, infant feeding patterns, antibiotic usage, sanitary living conditions and long term dietary habits contribute to shaping the composition of the gut microbiome. This review focuses primarily on diet, as it is one of the most pivotal factors in the development of the human gut microbiome from infancy to the elderly. The infant gut microbiota is characterized by a high degree of instability, only reaching a state similar to that of adults by 2–3 years of age; consistent with the establishment of a varied solid food diet. The diet-related factors influencing the development of the infant gut microbiome include whether the child is breast or formula-fed as well as how and when solid foods are introduced. In contrast to the infant gut, the adult gut microbiome is resilient to large shifts in community structure. Several studies have shown that dietary changes induce transient fluctuations in the adult microbiome, sometimes in as little as 24 h; however, the microbial community rapidly returns to its stable state. Current knowledge of how long-term dietary habits shape the gut microbiome is limited by the lack of long-term feeding studies coupled with temporal gut microbiota characterization. However, long-term weight loss studies have been shown to alter the ratio of the Bacteroidetes and Firmicutes, the two major bacterial phyla residing in the human gastrointestinal tract. With aging, diet-related factors such as malnutrition are associated with microbiome shifts, although the cause and effect relationship between these factors has not been established. Increased pharmaceutical usage is also more prevalent in the elderly and can contribute to reduced gut microbiota stability and diversity. Foods containing prebiotic oligosaccharide components that nurture beneficial commensals in the gut community and probiotic supplements are being explored as interventions to manipulate the gut microbiome, potentially improving health status.

show abstract

Section: The Aging Gutmentioning

confidence: 99%

Diet and the development of the human intestinal microbiome

2014

View full text Add to dashboard Cite

show abstract

“…The identification of adhesion mechanisms and molecules is a fundamental step in the elucidation of the bacterium/host cross-talk (van de Guchte et al, 2012). This was lacking in probiotic dairy propionibacteria.…”

Section: Introductionmentioning

confidence: 99%

Propionibacterium freudenreichii Surface Protein SlpB Is Involved in Adhesion to Intestinal HT-29 Cells

et al. 2017

Self Cite

View full text Add to dashboard Cite

Propionibacterium freudenreichii is a beneficial bacterium traditionally used as a cheese ripening starter and more recently for its probiotic abilities based on the release of beneficial metabolites. In addition to these metabolites (short-chain fatty acids, vitamins, and bifidogenic factor), P. freudenreichii revealed an immunomodulatory effect confirmed in vivo by the ability to protect mice from induced acute colitis. This effect is, however, highly strain-dependent. Local action of metabolites and of immunomodulatory molecules is favored by the ability of probiotics to adhere to the host cells. This property depends on key surface compounds, still poorly characterized in propionibacteria. In the present study, we showed different adhesion rates to cultured human intestinal cells, among strains of P. freudenreichii. The most adhesive one was P. freudenreichii CIRM-BIA 129, which is known to expose surface-layer proteins. We evidenced here the involvement of these proteins in adhesion to cultured human colon cells. We then aimed at deciphering the mechanisms involved in adhesion. Adhesion was inhibited by antibodies raised against SlpB, one of the surface-layer proteins in P. freudenreichii CIRM-BIA 129. Inactivation of the corresponding gene suppressed adhesion, further evidencing the key role of slpB product in cell adhesion. This work confirms the various functions fulfilled by surface-layer proteins, including probiotic/host interactions. It opens new perspectives for the understanding of probiotic determinants in propionibacteria, and for the selection of the most efficient strains within the P. freudenreichii species.

show abstract

“…The pool of proteins produced by a microorganism at certain growth stage is highly influenced by environmental factors and therefore, production of key proteins for probiotics adaptation and functionality may vary under specific conditions (Table 2). Proteomics helps discern probiotic attributes modulation by environmental factors [31,105], but also their effect on the intestinal cells proteome and gut metaproteome [106].…”

Section: Proteomics For the Study Of Changes In Probiotic Functionalitymentioning

confidence: 99%

Tackling probiotic and gut microbiota functionality through proteomics

Ruíz

Hidalgo

Blanco-Míguez

et al. 2016

Journal of Proteomics

View full text Add to dashboard Cite

Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. Molecular basis underlying the functional properties of probiotic bacteria responsible for the health promoting effects have been in the background for many years. Breakthrough of omics technologies in the probiotic and microbiota fields has had a very relevant impact in the elucidation of probiotic mechanisms and in the procedures to select these microorganisms, based on solid scientific evidence. It is unquestionable that, in the near future, the evolution of proteomic techniques will play a pivotal role in the generation of knowledge about the functions of probiotics and gut commensals, still a pending issue in the field of intestinal microbiomics.

show abstract

Properties of probiotic bacteria explored by proteomic approaches

Cited by 17 publications

References 73 publications

Diet and the development of the human intestinal microbiome

Diet and the development of the human intestinal microbiome

Propionibacterium freudenreichii Surface Protein SlpB Is Involved in Adhesion to Intestinal HT-29 Cells

Tackling probiotic and gut microbiota functionality through proteomics

Contact Info

Product

Resources

About