Properties of plasma membrane H+-ATPase in salt-treated Populus euphratica callus

Abstract

The plasma membrane (PM) vesicles from Populus euphratica (P. euphratica) callus were isolated to investigate the properties of the PM H(+)-ATPase. An enrichment of sealed and oriented right-side-out PM vesicles was demonstrated by measurement of the purity and orientation of membrane vesicles in the upper phase fraction. Analysis of pH optimum, temperature effects and kinetic properties showed that the properties of the PM H(+)-ATPase from woody plant P. euphratica callus were consistent with those from herbaceous species. Application of various thiol reagents to the reaction revealed that reduced thiol groups were essential to maintain the PM H(+)-ATPase activity. In addition, there was increased H(+)-ATPase activity in the PM vesicles when callus was exposed to NaCl. Western blotting analysis demonstrated an enhancement of H(+)-ATPase content in NaCl-treated P. euphratica callus compared with the control.

The plasma membrane H+-ATPase is related to the development of salicylic acid-induced thermotolerance in pea leaves

“…Recently, a lot of research focused on the reception and amplification of heat signals and the expression of specific genes to synthesize proteins and metabolites against heat injury. In this study, the protein with molecular mass of 100 kDa was specifically recognized in heat-acclimated leaves and PAC-pretreated pea leaves, which were in agreement to the results of Zhang et al (2006) and Yang et al (2007). Sung et al (1999) have shown that cold treatment stimulated the expression of Ps H ?…”

“…-ATPase was identified from salt-stressed expanded leaf tissue of tomato (Kalampanayil and Wimmers 2001). In line with this, Yang et al (2007) demonstrated the increase of activity of PM H ? -ATPase and an enhancement of H ?…”

“…It is also modulated by calcium ions, abscisic acid, auxin, cGMP, light, phospholipids and kinases (Kinoshita et al 1995;Michelet and Boutry 1995;Suwastika and Gehring 1999;Peng et al 2003). Furthermore, the activity and gene expression of this enzyme are affected by various environmental factors, such as phosphorus starvation (Shen et al 2006), Al stress (Ahn et al 2001) and salinity (Vitart et al 2001;Yang et al 2007). An induced PM H ?…”

The plant plasma membrane proton pump ATPase: a highly regulated P-type ATPase with multiple physiological roles

“…However, plants are able to extrude Na + through a Na + /H + antiporter (SOS1 in Arabidopsis), the activity of which depends on the pH gradient generated across the plasma membrane by H + -ATPase (for review see [47]). Exposure of Populus euphratica callus or Medicago to salt led to increased H + -ATPase expression and activity [48,49]. In Arabidopsis, analysis of the aha4-1 line encoding a truncated AHA4 mRNA indicated that this line was more sensitive to NaCl stress, resulting in a four-to fivefold higher root Na + /K + ratio than in the wild type; however, it was not possible to determine whether this phenotype was related to a dominant negative effect or to a gene-silencing effect [44].…”

Effect of NaCl on leaf H+-ATPase and the relevance to salt tolerance in two contrasting poplar species

“…Evidence by Western blotting proved that the expression of PM H ? -ATPase was up-regulated by NaCl stress in calluses and leaf tissues of P. euphratica (Yang et al 2007b;Zhang et al 2007). However, the localisation of H ?…”