Properties of pectinesterase from Penicillium fellutanum Biourge and new developments in pectin applications

Айзенберг, В. Л.; Syrchin, S. A.; Sedina, S.A.; Vasil'ev, V N; Shinkarenko, L. N.; Demchenko, P. I.; Vitte, P.N.

doi:10.1016/s0921-0423(96)80338-8

Cited by 2 publications

(2 citation statements)

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“…5). Data was almost agreed with Aizenberg et al, (1996) stated that the stability of PME at pH range (4-5). However, Christgau, et al, (1996) mentioned that PME of A. aculeatus was found most stable at pH 5.5-6.…”

Section: Optimum Ph Of Pme Activitysupporting

confidence: 84%

“…After 30 minutes of incubation at 55, 60, 65, 70 and 75°C, it lost 13, 32, 41, 89 and 100% of the original activity comparing with 18, 26, 37, 83 and 100 % after 20 min and 20, 23, 35, 77 and 96% after 10 minutes of incubation, respectively. Aizenberg et al, (1996) and Arbaisah et al, (1997) reported that PME was stable at temperatures less than 40°C. Both authors indicated that, for temperatures above 40°C, inactivation was notable after a short period of incubation.…”

Section: Optimum Ph Of Pme Activitymentioning

confidence: 99%

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Characterization of Pectin Methylesterase from Aspergillus niger

Al-Jammaas¹,

Sultan²,

Mahmood³

2023

TJAS

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The present study was undertaken to evaluate the characteristics of pectin methylesterase produced from Aspergillus niger. The enzyme had a molecular weight of 59.668 kDa. The enzyme exhibited maximum activity at 45°C. The enzyme lost most of its initial activity after 20 min of incubation at 75°C. Optimal activity and stability of the enzyme occurred at pH 4, with more than 90 % of retained activity after 10 min at pH 5. Michaelis constant (Km) was found equal to (1) mg/ml, whereas, maximum velocity (Vmax) was (1000) μmol/min. The catalyzed reaction by pectin methylesterase had activation energy equal to 9.66 Kcal/mol. The enzyme lost 24% and 13% of its initial activity during two months of storage at4°C and -20 °C, respectively. The enzyme was activated by NaCl, KCl and CaCl2 at concentration of 25 mM and inhibited by MgSO4, polygalacturonic acid, methanol, IAA and EDTA.

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Section: Optimum Ph Of Pme Activitysupporting

confidence: 84%

Section: Optimum Ph Of Pme Activitymentioning

confidence: 99%