Properties of Liposomes Prepared with Various Lipids

Hsieh, Y. F.; Chen, T.-L.; Wang, Y.-T.; Chang, Jer-Haur; Chang, Hung-Chi

doi:10.1111/j.1365-2621.2002.tb08820.x

Cited by 54 publications

(40 citation statements)

References 22 publications

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“…24,25 The high entrapment efficiency of PG-containing liposomes with respect to temperature exposure was reported previous- ly. 26,27 In general, instability of liposomes was attributed to collisions and eventual merging of liposomal membranes of two or more liposomes.…”

Section: Discussionmentioning

confidence: 65%

Characterization of Antimicrobial-bearing Liposomes by ζ-Potential, Vesicle Size, and Encapsulation Efficiency

et al. 2007

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Liposome entrapment may improve activity of protein or polypeptide antimicrobials against a variety of microorganisms. In this study, ability of liposomes to withstand exposure to environmental and chemical stresses typically encountered in foods and food processing operations were tested. Liposomes consisting of distearoylphosphatidylcholine (PC) and distearoylphosphatidylglycerol (PG), with 0, 5, or 10 μg/ml of the antimicrobial peptide nisin entrapped, were exposed to elevated temperatures (25-75°C) and a range of pH (5.5-11.0). Ability of liposomes to maintain integrity was assessed by measuring the encapsulation efficiency (EE), z-potential, and particle size distribution of liposomes. Distearoylphosphatidylcholine, PC/PG 8:2, and PC/PG 6:4 (mole fraction) liposomes retained between~70-90% EE despite exposure to elevated temperature and alkaline or acidic pH. Particle size of liposomes averaged between 100 and 240 nm depending on liposome preparation. Liposomal surface charge depended primarily on phospholipid composition and changed little with inclusion of nisin. Surface charge was not affected by temperature for PC and PC/PG 8:2 but decreased for PC/PG 6:4 liposomes. Our results suggest that liposomes containing nisin may be suitable for use as antimicrobial-active ingredients in low-or high-pH foods subjected to moderate heat treatments.

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Section: Discussionmentioning

confidence: 65%

Characterization of Antimicrobial-bearing Liposomes by ζ-Potential, Vesicle Size, and Encapsulation Efficiency

et al. 2007

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“…In the present study, we have investigated the use of stearic acid and cocoa butter as alternative stabilizers to cholesterol. Stearic acid was already tested in liposomal formulations [35,36]; however only the encapsulation efficiency was assessed and no investigation on long-term stability has been carried out. Cocoa butter has never been used in a liposome preparation; it was choosen since it is a widely used excipient in pharmacy and exhibits a better biocompatibility and lower in-vivo toxicity than semi-synthetic lipids.…”

Section: Choice Of the Stabilizermentioning

confidence: 99%

Preparation of liposomes: a novel application of microengineered membranes - investigation of the process parameters and application to the encapsulation of vitamin E

et al. 2013

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Liposomes with a mean size of 59-308 nm suitable for pulmonary drug delivery were prepared by the ethanol injection method using nickel microengineered flat disc membranes with a uniform pore size of 5-40 mm and a pore spacing of 80 or 200 mm. An ethanolic phase containing 20-50 mg ml 21 phospholipid (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) or Lipoid1 E80), 5-12.5 mg ml 21 stabilizer (cholesterol, stearic acid or cocoa butter), and 0 or 5 mg ml 21 vitamin E was injected through the membrane into an agitated aqueous phase at a controlled flux of 142-355 l m 22 h 21 and a shear stress on the membrane surface of 0.80-16 Pa. The mean particle size obtained under optimal conditions was 84 and 59 nm for Lipoid E80 and POPC liposomes, respectively. The particle size of the prepared liposomes increased with an increase in the pore size of the membrane and decreased with an increase in the pore spacing. Lipoid E80 liposomes stabilized by cholesterol or stearic acid maintained their initial size within 3 months. A high entrapment efficiency of 99.87% was achieved when Lipoid E80 liposomes were loaded with vitamin E. Transmission electron microscopy images revealed spherical multi-lamellar structure of vesicles. The reproducibility of the developed fabrication method was high.

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“…The electrostatic interaction of nisin with negatively charged membrane phospholipids is more pronounced than its interaction with neutral phospholipids [25]. Cationic vesicles containing stearylamine showed lower encapsulation efficiency when compared with other kind of vesicles, which could result from electrostatic repulsion between positively charged nisin and cationic vesicles [35]. The functional properties of liposomal nisin depend on the interaction of nisin with the liposome membrane and with the bacterial cell membrane.…”

Section: Characteristics Of Liposome-encapsulated Nisinmentioning

confidence: 97%

“…Their formation is based on the interactions between phospholipids and water molecules in which the polar headgroups of phospholipids are exposed to the inner and outer aqueous phases and the hydrophobic carbohydrate tails are forced to face each other in a bilayer [12,35]. Liposomal encapsulation has been shown to stabilize the encapsulated compound against enzymatic degradation and chemical modification [36].…”

Section: Liposomes As a Tool For Preventive Medicinementioning

confidence: 99%

Prospects for Liposome-Encapsulated Nisin in the Prevention of Dental Caries

Tsumori

Shimizu²,

Nagatoshi³

et al. 2015

Interface Oral Health Science 2014

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Dental caries is a common oral bacterial infectious disease. Its prevention requires the control of the causative pathogens, such as Streptococcus mutans, that exist within dental plaque. Nisin is a proteinaceous bacteriocin produced by Lactococcus lactis that is used to suppress bacterial infections. It has an inhibitory mode of action on a wide range of gram-positive bacteria. Improvements in the medical benefits of antibacterial agents can be achieved if they can be retained in liposomes for a long period after administration. Liposome systems can increase the ability of the encapsulated compounds, which have been widely used to encapsulate many kinds of compounds in various scientific settings. Liposomes can release labile molecules at a moderate rate. Liposome technologies that effectively protect the encapsulated molecules from decomposition have the potential to improve their preventive and therapeutic effects. Therefore, the use of liposomes to administer antimicrobial agents has spurred research into their utility in preventive medicine. The encapsulation of nisin in liposomes can provide means of improving the stability of nisin and its antibacterial effect against S. mutans. The present chapter will review the prospects for liposome-encapsulated nisin for the prevention of oral infectious diseases.

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Properties of Liposomes Prepared with Various Lipids

Cited by 54 publications

References 22 publications

Characterization of Antimicrobial-bearing Liposomes by ζ-Potential, Vesicle Size, and Encapsulation Efficiency

Characterization of Antimicrobial-bearing Liposomes by ζ-Potential, Vesicle Size, and Encapsulation Efficiency

Preparation of liposomes: a novel application of microengineered membranes - investigation of the process parameters and application to the encapsulation of vitamin E

Prospects for Liposome-Encapsulated Nisin in the Prevention of Dental Caries

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