Properties of heparin binding to purified plasma membranes from bovine granulosa cells

Winer, Martin A.; Ax, R. L.

doi:10.1530/jrf.0.0870337

Cited by 10 publications

(8 citation statements)

References 30 publications

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“…Furthermore, since CXCL17 had no effect in membrane preparations this suggested the involvement of a site that was lost once intact cells were disrupted and pointed to the involvement of an accessory protein. Here we present several lines of evidence that this potential accessory protein contains glycosaminoglycans: 1) CXCL17 contains multiple highly conserved putative GAG binding domains and preparation of purified cell membranes certainly disrupts the native structure, function and/or potentially presence of GAGs 41 . 2) In support of this, the effect of CXCL17 was mimicked by known GAG binders, surfen and protamine sulphate.…”

Section: Discussionmentioning

confidence: 99%

CXCL17 is an endogenous inhibitor of CXCR4 via a novel mechanism of action

White

Kilpatrick

Dale

et al. 2021

Preprint

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CXCL17 is the most recently described chemokine. It is principally expressed by mucosal tissues, where it facilitates chemotaxis of monocytes, dendritic cells, and macrophages and has antimicrobial properties. CXCL17 is also implicated in the pathology of inflammatory disorders and progression of several cancers, as well as being highly upregulated during viral infections of the lung. However, the exact role of CXCL17 in health and disease is largely unknown, mainly due to a lack of known molecular targets mediating CXCL17 functional responses. Using a range of bioluminescence resonance energy transfer (BRET) based assays, here we demonstrate that CXCL17 inhibits CXCR4-mediated signalling and ligand binding. Moreover, CXCL17 interacts with neuropillin-1, a VEGFR2 co-receptor. Additionally, we find CXCL17 only inhibits CXCR4 ligand binding in intact cells and demonstrate that this effect is mimicked by known glycosaminoglycan binders, surfen and protamine sulfate. This indicates that CXCL17 inhibits CXCR4 by a unique mechanism of action that potentially requires the presence of a glycosaminoglycan containing accessory protein. Altogether, our results reveal that CXCL17 is an endogenous inhibitor of CXCR4 and represents an important discovery in our understanding of the (patho) physiological functions of CXCL17 and regulation of CXCR4 signalling.

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Section: Discussionmentioning

confidence: 99%

CXCL17 is an endogenous inhibitor of CXCR4 via a novel mechanism of action

White

Kilpatrick

Dale

et al. 2021

Preprint

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“…Aside from smooth muscle cells and endothelial cells, heparins also bind to various other cell types, including granulosa cells, 17 epithelial cells, 18,19 and melanoma cells. 20 Although there is no reference (to the best of our knowledge) to heparin-binding receptors on fibroblast cells, the presence of specific heparin-binding sites (receptors) on fibroblast cells is nonetheless expected.…”

Section: Discussionmentioning

confidence: 99%

Heparin-carrying polystyrene to mediate cellular attachment and growth via interaction with growth factors

Ishihara

Saitō²,

Yura³

et al. 2000

J. Biomed. Mater. Res.

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Various sugar-carrying polystyrenes (PSs), which consist of synthetic styrene and sugar moieties, are glycoconjugates that are able to attach to polymeric surfaces. Heparin-carrying PS (HCPS) is especially able to retain the binding of heparin-binding growth factors (GFs) such as vascular endothelial GF 165 (VEGF(165)) or fibroblast GF 2 (FGF-2). Human skin fibroblast cells, human coronary smooth muscle cells, and human coronary endothelial cells have good adherence to the HCPS-coated plate. The growth rate of fibroblast cells on HCPS-coated plates is higher than or comparable to fibronectin-coated, gelatin-coated, or tissue culture treated plates, and the HCPS coating inhibits the growth of smooth muscle cells. On the other hand, the growth rate of endothelial cells on HCPS-coated plates in the presence of either VEGF(165) or FGF-2 is comparable to that on fibronectin-coated, gelatin-coated, and tissue culture treated plates. Endothelial cells grow at a higher rate on HCPS-coated plates retained with either VEGF(165) or FGF-2 than on the other coated plates. These results indicate that growth of various cells can be controlled by the HCPS coating, thereby retaining the bioactivity of molecules such as heparin-binding GFs. Thus, HCPS-coated surfaces control selective growth of various cells.

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“…Thus, the additional interaction between heparin and phospholipid must have contributed to the intermolecular association of lipid-conjugated heparin and the interaction with the cell membrane to induce cell aggregation. It has been reported that heparin can attach to the cell membrane by interacting with membrane proteins − or by directly interacting with phospholipids . The intermolecular association among lipid-conjugated heparin molecules was evident from the formation of aggregates by lipid-conjugated heparins themselves.…”

Section: Resultsmentioning

confidence: 99%

Rapid and Versatile Cell Aggregate Formation Using Lipid-Conjugated Heparin

Kim

Min

et al. 2018

ACS Appl. Mater. Interfaces

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Cell aggregates hold significant therapeutic promise for in vitro cell analysis, ex vivo tissue models, and in vivo cell therapy and tissue engineering. Traditional methods of making cell aggregates require long incubation times and can only produce three-dimensional-spheroid-shaped aggregates. We propose a novel method of making cell aggregates of diverse sizes and shapes using lipid-conjugated heparin. Shaking the cell suspension containing a small amount of lipid-conjugated heparin for approximately 30 min produced cell aggregates. This approach can be applied to any cell type, including stem cells, fibroblast cells, and T lymphocytes. The shape of biocompatible templates could modulate the shape of cell aggregates. In addition to layered, multicompartmental cell aggregates on template, template-free, tube-shaped cell aggregates could also be made. The cell aggregates formed were alive and maintained biological activities.

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Properties of heparin binding to purified plasma membranes from bovine granulosa cells

Abstract: Summary. Bovine

Cited by 10 publications

References 30 publications

CXCL17 is an endogenous inhibitor of CXCR4 via a novel mechanism of action

CXCL17 is an endogenous inhibitor of CXCR4 via a novel mechanism of action

Heparin-carrying polystyrene to mediate cellular attachment and growth via interaction with growth factors

Rapid and Versatile Cell Aggregate Formation Using Lipid-Conjugated Heparin

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