Properties of cellulases fromSclerotium rolfsii

Darmwal, N. S.; Gaur, Anshu

doi:10.1007/bf01741767

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1990

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“…However, exoglucanase (filter paper activity) of Sclerotium rolfsii (17) has been reported to be most active at pH 4.8. The optimum assay temperature (50°C) of purified exoglucanase was similar to that of S. rolfsii (6) and A. terreus GN-l (8).…”

Section: And Discussionmentioning

confidence: 69%

Properties of exoglucanase from Aspergillus niger.

Singh

Agrawal

Abidi

et al. 1990

J. Gen. Appl. Microbiol.

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Exoglucanase (EC 3.2.1.91) was partially purified from Aspergillus niger. The enzyme was stable at room temperature in the pH range of 4.0-6.0 for 24h, with the optimum at 5.5. The enzyme had an optimum temperature of 50°C and a t112 at 65°C was 70 min. Km and Vmax values were found to be 55.5 mg/ml and 0.9 ,uM/min, respectively. Glycerol protected the enzyme from inactivation on storage and from denaturation due to freezing and thawing. The effect of the sulfhydryl group reagents tested suggested the presence of -SH on the active site of the enzyme. Mn2+ and Co2+ were good activators of the enzyme, whereas Hg2 + and Pb2 + were potent inhibitors. The enzyme was a metalloprotein, or it requires certain metal ions for activation.

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Section: And Discussionmentioning

confidence: 69%