Properties of carbon nanotube-dispersed Sr-hydroxyapatite injectable material for bone defects

Raucci, Maria Grazia; Álvarez-Pérez, Marco Antonio; Giugliano, Daniela; Zeppetelli, S.; Ambrosio, Luigi

doi:10.1093/rb/rbv026

Cited by 26 publications

(12 citation statements)

References 51 publications

(48 reference statements)

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“…Meanwhile, lowest values of cell proliferation were detected for neat MeHA 30 and MeHA 30-DN, to the poor adhesive properties of hyaluronic acid. 49 Meanwhile, for the composite materials, the synergic effect of bioactive signal, due to HAp, exposed on the material surface 50 and improved mechanical properties, in terms of storage modulus and maximum strength, reported in Table II and Figure 8(C), induced an early differentiation of hMSC. 51 Here, an increase of ALP expression over culture time (3 and 7 days) for all composite materials was observed ( Fig.…”

Section: Biological Analysismentioning

confidence: 98%

Bioactive composites based on double network approach with tailored mechanical, physico‐chemical, and biological features

D’Amora

Ronca

Raucci

et al. 2018

J Biomedical Materials Res

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Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes. In this study, HA was chemically functionalized with photo-cross-linkable motifs by reacting with methacrylic anhydride (MA) to obtain methacrylated hyaluronic acid (MeHA). A range of MA/HA molar ratios was used to obtain different degrees of substitution (DS) ranging from 3.5% to 74.5%, as showed by nuclear magnetic resonance and attenuated total reflection spectroscopy. By fine tuning the DS, the chemical reaction parameters, and the polymer concentration, it was demonstrated the possibility to tailor their mechanical features. Double network (DN) hydrogels were prepared through the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA). To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation. The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium. This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3079-3089, 2018.

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Section: Biological Analysismentioning

confidence: 98%

Bioactive composites based on double network approach with tailored mechanical, physico‐chemical, and biological features

D’Amora

Ronca

Raucci

et al. 2018

J Biomedical Materials Res

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“…Such substitutions are accompanied by changes in network parameters and unit cell volume, which are generally related to the size of the cation ionic radius compared to that of Ca 2+ [238]. Most commonly used cations to replace calcium ions are zinc (Zn 2+ ) [239], strontium (Sr 2+ ) [240], magnesium (Mg 2+ ), and manganese (Mn 2+ ). Other materials have been studied, such as silicate (SiO 4…”

Section: -mentioning

confidence: 99%

A brief review on hydroxyapatite production and use in biomedicine

et al. 2019

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Hydroxyapatite (HAp) is a bioceramic widely studied due to its chemical similarity with the mineral component of bones. Besides, it is biocompatible, bioactive and thermodynamically stable in the body fluid what poses it as an attractive material for a wide range of applications in the biomedical field. Several efforts have been focused on the synthesis of particles of this material aiming to the precise control of size and morphology, porosity and surface area. HAp is widely used as an implant for bone tissue regeneration, as a coating for metallic implants and in a drug-controlled release. In this sense, the objective of this review is to gather information related to HAp, providing readers with information about synthesis methods, material characteristics and their applications.

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“…Autologous graft is known as the gold standard in the bone replacement, but is concomitant with shortcomings such as limited graft quantity and donor site morbidity [ 3 ]. Accordingly, various synthetic bone graft substitutes including metallic implants [ 4 ], bioactive glasses [ 5 ] and ceramics [ 6 , 7 ], and bone-like composites [ 8 , 9 ], have been studied in order to meet the clinical need for reconstruction of bone deficiencies. Systematic in vitro and in vivo assessments are generally involved in these studies.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the osteogenic capability of rat bone mesenchymal stem cells on collagen, collagen/hydroxyapatite, hydroxyapatite and biphasic calcium phosphate

Sun

et al. 2017

Regenerative Biomaterials

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Collagen (COL), collagen/hydroxyapatite (COL/HA), HA and biphasic calcium phosphate were prepared as representative bone grafting materials with composition analogous to bone, and their structural characteristics were analyzed. The rat bone mesenchymal stem cells (BMSCs) were further seeded onto four groups of materials, and BMSCs grown in basic medium and standard osteogenic medium were set as controls of a reference model to show the basic and osteogenic behavior of cells without the intervention of materials. Cellular behaviors were characterized, including proliferation, spreading morphology and expression of osteogenesis factors. The rat BMSCs proliferated properly with time on four groups of materials as well on two groups of controls, and typical cuboidal, polygonal and extremely-elongated morphologies of cells were observed. According to the real-time polymerase chain reaction data, a higher osteogenic gene expression level was dependent upon the growing morphology but not the proliferation rate of cells, and the osteogenic differentiation capacity of cells onto four groups of materials varied in specific genes. In general, BMSCs exhibited the highest osteogenic capacity onto COL/HA, but the poorest onto HA. The growing behaviors of cells on materials were further discussed in comparison with the cases of OC and BC of the reference model. The present attempt to comparatively analyze cell experimental data with a reference model is expected to be useful for revealing the difference in the osteogenic capability of MSCs onto materials or even the bioactivity of materials.

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Properties of carbon nanotube-dispersed Sr-hydroxyapatite injectable material for bone defects

Cited by 26 publications

References 51 publications

Bioactive composites based on double network approach with tailored mechanical, physico‐chemical, and biological features

Bioactive composites based on double network approach with tailored mechanical, physico‐chemical, and biological features

A brief review on hydroxyapatite production and use in biomedicine

Comparison of the osteogenic capability of rat bone mesenchymal stem cells on collagen, collagen/hydroxyapatite, hydroxyapatite and biphasic calcium phosphate

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