Properties of broad bean yellow band virus, a possible new tobravirus

Russo, Marcello; Gallitelli, D.; Vovlas, C.; Savino, V.

doi:10.1111/j.1744-7348.1984.tb03046.x

Cited by 13 publications

(16 citation statements)

References 15 publications

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“…Robinson, personal communication). These plants were also resistant to BBYBV, which has substantial nucleotide sequence homology with PEBV but is serologically unrelated (22,23). However, 54.3 plants were not resistant to infection by either PRV (24) or the 16 isolate of TRV (25).…”

Section: Resultsmentioning

confidence: 99%

Plants transformed with a region of the 201-kilodalton replicase gene from pea early browning virus RNA1 are resistant to virus infection.

MacFarlane

Davies²

1992

Proc. Natl. Acad. Sci. U.S.A.

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The 3' proximal portion of the gene encoding the 201-kDa putative replicase protein from the Tobravirus pea early browning virus (PEBV) can potentially be expressed separately as a 54-kDa protein. Nicoiana benthamiana plants transformed with the open reading frame (ORF) encoding the 54-kDa protein, designated 54K ORF, were resistant to infection by purified PEBV at inoculum doses of up to 1 mg/mi, the highest concentration tested. However, resistance was abolished by the introduction into the 54K ORF of mutations that would cause premature termination of translation. This suggests that the resistance mechanism requires the involvement of an intact 54-kDa protein. The 54K ORF-transformed plants were also resistant to infection by broad bean yellow band virus and an uncharacterized isolate of British PEBV (PGRO R) but were not resistant to infection by two other tobraviruses, pepper ringspot virus and the 16 isolate of tobacco rattle virus. Additionally, two variants of PEBV which overcame 54K ORF-mediated resistance have been isolated, the analysis of which might provide important information about both the resistance mechanism itself and the process of normal virus replication.Pea early browning virus (PEBV) is a member of the Tobravirus group of plant viruses, which have a genome consisting of two positive-sense RNAs separately encapsidated in rodshaped particles. Analysis of the sequence of the viral RNAs revealed that the genetic organization of the larger RNA (RNA1) of PEBV resembles that of the monopartite virus tobacco mosaic virus (TMV) (1, 2). PEBV RNA1 encodes two proteins with molecular masses of 141 and 201 kDa, which share amino acid sequence homology with the TMV 126-kDa and 183-kDa replicase proteins. These proteins are NH2-coterminal, the larger protein being expressed by the read-through of an opal (UGA) termination codon at the COOH terminus of the 141-kDa protein (1,3). Additionally, PEBV RNA1 encodes a 30-kDa protein with homology to the TMV 30-kDa cell-to-cell spread protein. However, whereas the 3' proximal gene of TMV encodes the virus coat protein, this region in PEBV RNA1 encodes a cysteine-rich 12-kDa protein. PEBV coat protein is encoded by the 5' proximal gene of RNA2, which also encodes putative 29.6-kDa and 23-kDa proteins of unknown function (4).Closer inspection of the PEBV RNA1 sequence revealed that the COOH-terminal region of the 201-kDa protein could potentially be expressed separately as a 54-kDa protein by translation of an internal open reading frame (ORF) initiating at an AUG codon situated 147 nucleotides (encoding 49 amino acids) downstream of the termination codon (nucleotide 3866) for the 141-kDa protein. The PEBV ORF encoding the 54-kDa protein, designated 54K ORF,t encodes amino acid sequences that are highly conserved among the RNAdependent RNA polymerases from a wide range of plant, animal, and bacterial viruses. Several studies have presented detailed alignments of these proteins and identified a number of highly conserved sequence motifs that presumably ar...

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Section: Resultsmentioning

confidence: 99%

Plants transformed with a region of the 201-kilodalton replicase gene from pea early browning virus RNA1 are resistant to virus infection.

MacFarlane

Davies²

1992

Proc. Natl. Acad. Sci. U.S.A.

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“…N5 is the isolate from narcissus plant N5, described by Harrison et al (1983). The isolates of PEBV used were SHE (PEBV-SHE) and SP5 (PEBV-SP5), which are isolates of the British serotype from pea (Robinson & Harrison, 1985a), El 16, an isolate of the Dutch serotype from pea (kindly supplied by L. Bos; PEBV-D) and BBYBV, an isolate from Viciafaba in Italy (Russo et aL, 1984;PEBV-BBYB). Unless otherwise stated, TRV was strain SYM (Kurppa et al, 1981); other strains were as used by Robinson & Harrison (1985a).…”

Section: Methodsmentioning

confidence: 99%

Two Anomalous Tobravirus Isolates: Evidence For RNA Recombination in Nature

Robinson

Hamilton²,

Harrison

et al. 1987

Journal of General Virology

100

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SUMMARY16 and N5 are naturally occurring tobravirus isolates that produce symptoms in herbaceous plants similar to those induced by strains of tobacco rattle virus (TRV). In immunosorbent electron microscopy tests, however, they reacted with antisera to particles of pea early-browning virus (PEBV), not TRV. Furthermore, these tests indicated that 16 was related to the British serotype of PEBV and N5 to the Dutch. Pseudo-recombinant isolates were produced by reassortment of the genome parts of 16 or N5 with those of TRV, in any combination, but not in most combinations with those of PEBV. However, I6 RNA-2 was replicated in plants inoculated also with RNA-1 from an isolate of the British serotype of PEBV, but the PEBV RNA-1 was imperfectly packaged by 16 coat protein, and the virus particles seemed to have only limited stability. Nucleic acid hybridization experiments showed that the RNA-1 sequences of both I6 and N5 were similar to those of TRV strains. I6 RNA-2 contained sequences resembling those of the British serotype of PEBV, but with some TRV-like sequences at the 3' and 5' ends, whereas N5 RNA-2 contained more extensive TRV-like 3' and 5' ends flanking sequences that were related, but perhaps not closely, to those of the Dutch serotype of PEBV. Thus, the RNA-2 species of 16 and N5 were recombinant molecules that contained sequences typical of both TRV and PEBV, and which probably had separate but similar evolutionary origins. As a result of their hybrid nature, I6 and N5 were part of the gene pool and had the pathogenicity of TRV, while possessing the serological properties of PEBV.

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“…BBYBV RNA (Russo et aL, 1984) was a gift from Dr M. Russo, Dipartimento di Patologia Vegetale, Bari, Italy; tobacco mosaic and cowpea mosaic virus (TMV and CPMV) RNAs were kindly supplied by Drs T. M. A. Wilson and G. P. Lomonossoff (John Innes Institute) respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Broad bean yellow band virus (BBYBV) has genomic RNAs of Mr 2"48 × 106 (RNA-1) and 1"06 × 106 (RNA-2) (Russo et al, 1984) which can form pseudorecombinants with PEBV RNA (Robinson & Harrison, 1985b), which suggests that the two viruses are closely related. BBYBV RNA-2 encodes a coat protein of approx.…”

Section: Introductionmentioning

confidence: 99%

In vitro Translation of the Bipartite Genomic RNA of Pea Early Browning Virus

Hughes

Davies

Wood

1986

Journal of General Virology

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SUMMARYTranslation of the two genomic RNAs of a British strain (SP5) of pea early browning virus (PEBV-B) in rabbit reticulocyte lysates produced three principal polypeptides of Mr 165 × 103 (165K), 134K and 23K. The two largest polypeptides were produced by RNA-1 alone, and evidence is presented which suggests that the 165K polypeptide arises from the 134K product by a readthrough event. RNA-2 directed the translation of the 23K polypeptide, which was immunoprecipitated by anti-PEBV-B serum.

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Properties of broad bean yellow band virus, a possible new tobravirus

Cited by 13 publications

References 15 publications

Plants transformed with a region of the 201-kilodalton replicase gene from pea early browning virus RNA1 are resistant to virus infection.

Plants transformed with a region of the 201-kilodalton replicase gene from pea early browning virus RNA1 are resistant to virus infection.

Two Anomalous Tobravirus Isolates: Evidence For RNA Recombination in Nature

In vitro Translation of the Bipartite Genomic RNA of Pea Early Browning Virus

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