Properties of bacterial cellulose developed from genetically stable FM833 bacterial strain for 30 generations via Kombucha tea

Abstract

The quality control of the bacterial cellulose (BC) production has been facing challenges due to the genetic stability of bacterial strains, which is often overlooked or underestimated. Thus, its commercialized application is limited. Therefore, a bacterial strain FM883 with a 16S rRNA sequence 97% identical to Komagataeibacter xylinus NBRC15237 T was isolated from kombucha tea. Cultivation of FM883 was conducted in series to observe the morphology and BC yield among different generations from 1 st generation to 30 th generation. The results show that FM883 can e ciently produce BC in modi ed Hestrin-Schramn media at the 30 th generation (163.5 ± 0.3 g/L), which was even higher than the 1 st generation (157.6 ± 0.3 g/L). This indicated that FM883 has strong genetic stability. Furthermore, several characterizations of the BC indicated that the quality of the BC biosynthesized by FM883 was good with excellent thermostability. With such strong genetic stability, properties, su ciently high consistency and high yield, BC biosynthesized by FM883 had demonstrated excellent production quality control, which is desired for commercial manufacturing.