Properties of Arsenite Efflux Permeases (Acr3) from Alkaliphilus metalliredigens and Corynebacterium glutamicum

Fu, Hseuh-Liang; Meng, Yuling; Ordóñez, Efrén; Villadangos, Almudena Fernández; Bhattacharjee, Hiranmoy; Gil, José A.; Mateos, Luís M.; Rosen, Barry P.

doi:10.1074/jbc.m109.011882

Cited by 85 publications

(94 citation statements)

References 33 publications

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“…Two families of transmembrane efflux pumps are known: the ArsB and the ACR3 family. The ACR3 type is more widespread in nature being found in prokaryotes, animals and plants (Fu et al 2009), remarkably in arsenic hyper accumulating plant Pteris vittata. ACR3(2) in particular, has been found in Alphaproteobacteria (Achour et al 2007) and in Betaproteobacteria (Pohlmann et al 2006;Bachate et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Rhizosphere colonization and arsenic translocation in sunflower (Helianthus annuus L.) by arsenate reducing Alcaligenes sp. strain Dhal-L

Cavalca

Corsini

Bachate

et al. 2013

World J Microbiol Biotechnol

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In the present study, six arsenic-resistant strains previously isolated were tested for their plant growth promoting characteristics and heavy metal resistance, in order to choose one model strain as an inoculum for sunflower plants in pot experiments. The aim was to investigate the effect of arsenic-resistant strain on sunflower growth and on arsenic uptake from arsenic contaminated soil. Based on plant growth promoting characteristics and heavy metal resistance, Alcaligenes sp. strain Dhal-L was chosen as an inoculum. Beside the ability to reduce arsenate to arsenite via an Ars operon, the strain exhibited 1-amino-cyclopropane-1-carboxylic acid deaminase activity and it was also able to produce siderophore and indole acetic acid. Pot experiments were conducted with an agricultural soil contaminated with arsenic (214 mg kg -1 ). A real time PCR method was set up based on the quantification of ACR3(2) type of arsenite efflux pump carried by Alcaligenes sp. strain Dhal-L, in order to monitor presence and colonisation of the strain in the bulk and rhizospheric soil. As a result of strain inoculation, arsenic uptake by plants was increased by 53 %, whereas ACR3(2) gene copy number in rhizospheric soil was 100 times higher in inoculated than in control pots, indicating the colonisation of strain. The results indicated that the presence of arsenate reducing strains in the rhizosphere of sunflower influences arsenic mobilization and promotes arsenic uptake by plant.

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Section: Introductionmentioning

confidence: 99%

Rhizosphere colonization and arsenic translocation in sunflower (Helianthus annuus L.) by arsenate reducing Alcaligenes sp. strain Dhal-L

Cavalca

Corsini

Bachate

et al. 2013

World J Microbiol Biotechnol

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“…Members of the Acr3 family are found in bacteria, archaea and fungi (Rosen, 1999;Wysocki et al, 2003) and in environmental studies acr3 genes seem to be very widespread among arsenic resistant bacteria, even more than arsB genes (Achour et al, 2007;Cai et al, 2009). Nevertheless, Acr3 proteins have been functionally characterized only in a few species including Bacillus subtilis, Synechocystis sp., Corynebacterium glutamicum and Saccharomyces cerevisiae (López-Maury et al, 2003;Aaltonen and Silow, 2008;Fu et al, 2009;MaciaszczykDziubinska et al, 2011;Villadangos et al, 2012), the last being the best studied microorganism where Acr3 acts as a metalloid/H(+) antiporter for arsenite and antimonite. Poirel et al suggest that arsB and acr3, arsenite transporter genes, are appropriate biomarkers of arsenic stress that may be suitable for further exploring the adaptive response of bacterial communities to arsenic in contaminated environments (Poirel et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Genome comparison of two Exiguobacterium strains from high altitude andean lakes with different arsenic resistance: identification and 3D modeling of the Acr3 efflux pump

Ordoñez

Lanzarotti

Kurth

et al. 2015

Front. Environ. Sci.

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Arsenic exists in natural systems in a variety of chemical forms, including inorganic arsenite (As [III]) and arsenate (As [V]). The majority of living organisms have evolved various mechanisms to avoid occurrence of arsenic inside the cell due to its toxicity. Common core genes include a transcriptional repressor ArsR, an arsenate reductase ArsC, and arsenite efflux pumps ArsB and Acr3. To understand arsenic resistance we have performed arsenic tolerance studies, genomic and bioinformatic analysis of two Exiguobacterium strains, S17 and N139, from the high-altitude Andean Lakes. In these environments high concentrations of arsenic were described in the water due to a natural geochemical phenomenon, therefore, these strains represent an attractive model system for the study of environmental stress and can be readily cultivated. Our experiments show that S17 has a greater tolerance to arsenite (10 mM) than N139, but similar growth in arsenate (150 mM). We sequenced the genome of the two Exiguobacterium and identified an acr3 gene in S17 as the only difference between both species regarding known arsenic resistance genes. To further understand the Acr3 we modeled the 3D structure and identified the location of relevant residues of this protein. Our model is in agreement with previous experiments and allowed us to identify a region where a relevant cysteine lies. This Acr3 membrane efflux pump, present only in S17, may explain its increased tolerance to As(III) and is the first Acr3-family protein described in Exiguobacterium genus.

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“…S1 in the supplemental material). ArsR is a trans-acting repressor involved in the regulation of the ars operon, ArsC is an arsenate reductase that reduces arsenate to arsenite prior to efflux, and Acr3 is a transmembrane antiporter (35)(36)(37) that pumps arsenite out of the cell.…”

Section: Resultsmentioning

confidence: 99%

Arsenic Detoxification by Geobacter Species

Dang

Walker

Vautour

et al. 2017

Appl Environ Microbiol

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Insight into the mechanisms for arsenic detoxification by Geobacter species is expected to improve the understanding of global cycling of arsenic in ironrich subsurface sedimentary environments. Analysis of 14 different Geobacter genomes showed that all of these species have genes coding for an arsenic detoxification system (ars operon), and several have genes required for arsenic respiration (arr operon) and methylation (arsM). Genes encoding four arsenic repressorlike proteins were detected in the genome of G. sulfurreducens; however, only one (ArsR1) regulated transcription of the ars operon. Elimination of arsR1 from the G. sulfurreducens chromosome resulted in enhanced transcription of genes coding for the arsenic efflux pump (Acr3) and arsenate reductase (ArsC). When the gene coding for Acr3 was deleted, cells were not able to grow in the presence of either the oxidized or reduced form of arsenic, while arsC deletion mutants could grow in the presence of arsenite but not arsenate. These studies shed light on how Geobacter influences arsenic mobility in anoxic sediments and may help us develop methods to remediate arsenic contamination in the subsurface. IMPORTANCE This study examines arsenic transformation mechanisms utilized byGeobacter, a genus of iron-reducing bacteria that are predominant in many anoxic iron-rich subsurface environments. Geobacter species play a major role in microbially mediated arsenic release from metal hydroxides in the subsurface. This release raises arsenic concentrations in drinking water to levels that are high enough to cause major health problems. Therefore, information obtained from studies of Geobacter should shed light on arsenic cycling in iron-rich subsurface sedimentary environments, which may help reduce arsenic-associated illnesses. These studies should also help in the development of biosensors that can be used to detect arsenic contaminants in anoxic subsurface environments. We examined 14 different Geobacter genomes and found that all of these species possess genes coding for an arsenic detoxification system (ars operon), and some also have genes required for arsenic respiration (arr operon) and arsenic methylation (arsM).KEYWORDS ars operon, Geobacter, iron reduction, detoxification, genetics, transcriptomics, arsenic A rsenic is a naturally occurring metalloid that is found in many minerals, usually in conjunction with such metals as iron, lead, nickel, copper, and cobalt (1). Both inorganic and organic arsenic forms are found in soils, sediments, water, and living organisms, with inorganic forms being most abundant (2, 3). In the environment, the oxidized form of inorganic arsenic [arsenate; As(V)] is relatively nonmobile as it strongly adsorbs to such minerals as ferrihydrite and alumina. The reduced form, arsenite [As(III)], on the other hand, does not adsorb well to many minerals, making it highly

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Properties of Arsenite Efflux Permeases (Acr3) from Alkaliphilus metalliredigens and Corynebacterium glutamicum

Cited by 85 publications

References 33 publications

Rhizosphere colonization and arsenic translocation in sunflower (Helianthus annuus L.) by arsenate reducing Alcaligenes sp. strain Dhal-L

Rhizosphere colonization and arsenic translocation in sunflower (Helianthus annuus L.) by arsenate reducing Alcaligenes sp. strain Dhal-L

Genome comparison of two Exiguobacterium strains from high altitude andean lakes with different arsenic resistance: identification and 3D modeling of the Acr3 efflux pump

Arsenic Detoxification by Geobacter Species

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