Properties of aqueous-saline human placental extracts and their fractions after storage of placenta at various subzero temperatures

Nardid, О. А.; Repina, S.V.; Rozanova, E. D.; Cherkashina, Yana; Nardid, E. O.

doi:10.5455/jeim.231115.or.141

Cited by 4 publications

(4 citation statements)

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“…All studies have been approved by the Bioethics Committee of the Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine and have been performed in accordance with the ethical standards stated in the 1964 'Declaration of Helsinki'. In our study we used total aqueous-saline extract obtained from fresh human placentas as well as its individual fractions, isolated by gel chromatography, as described by us previously (19). Human placentas, weighing between 400-600 g, were collected after normal pregnancies from healthy parturients (23-28 years old Caucasians) with their informed consent.…”

Section: Methodsmentioning

confidence: 99%

“…This ensured recording EPR signal exclusively from the probes which penetrated into cytoplasm. The EPR spin probe investigations were carried out as described by (19) with some modifications. Briefly, EPR spectra of TEMPON were recorded in the temperature range from 30 to 55°С using EPR spectrometer equipped with a thermostat maintaining temperature with accuracy of ± 0.5ºС.…”

Section: Cytosol Microviscosity and Membrane Barrier Function At Hypementioning

confidence: 99%

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Beneficial impact of human placenta extracts on erythrocyte membrane thermostability

Nardid¹,

Repina²,

Bobrova³

et al. 2018

TJS

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PURPOSE: To study the influence of human placenta extract (HPE) and its individual fractions on the thermal stability of human erythrocyte membrane. METHODS: HPE fractions were isolated by gel chromatography. Thermal hemolysis of erythrocytes, exposed to 55°C was measured spectrophotometrically. Cytosol microvscosity and barrier function of erythrocyte membranes at hyperthermia were investigated by EPR spin probe TEMPON. Thermal denaturation of erythrocyte membrane proteins were studied by differential scanning calorimetry. RESULTS: Pre-treatment of erythrocytes with HPE or its fractions inhibited thermal hemolysis. Low-molecular fractions (below 4 kDa and 12-20 kDa) were the most effective in thermal hemolysis inhibition ((31.7±3.3) % and (31.5±3.2) %, respectively). The latter fractions markedly reduced the hyperthermia (55°C)-induced permeability of erythrocytes for ferricyanide ions and inhibited the thermo-induced structural transitions in erythrocyte membrane between 40 and 50°C, which are associated with cytoskeletal proteins. HPE fractions reversibly increased the denaturation temperatures of erythrocyte membrane proteins, except that of spectrin, and enlarged the enthalpy of denaturation of all membrane proteins. CONCLUSIONS: HPE and its individual fractions increased the thermal stability of erythrocyte membranes and erythrocytes. This effect was attributed to the reversible binding of some low molecular ingredient of HPE to the integral proteins and consequent stabilization of their interaction with under-membrane cytoskeleton.

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Section: Methodsmentioning

confidence: 99%

Section: Cytosol Microviscosity and Membrane Barrier Function At Hypementioning

confidence: 99%

Beneficial impact of human placenta extracts on erythrocyte membrane thermostability

Nardid¹,

Repina²,

Bobrova³

et al. 2018

TJS

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“…Екстракти плаценти людини (ЕПЛ), які мають широкий спектр протеїнів, пептидів, мінералів, амінокислот, стероїдних гормонів тощо, також знайшли широке використання у медичній галузі [3]. Дослідження впливу низьких температур на біологічно-активні речовини водносольових екстрактів плаценти проводилися [4], проте це питання вивчено недостатньо.…”

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“…Відмивали у 6-кратному об'ємі фізіологічного розчину. Екстракти плаценти людини (ЕПЛ), як зі свіжоотриманих плацент, так і з їх заморожених-відігрітих фрагментів, одержували, як описано нами раніш [4]. Окремі фракції ЕПЛ отримували за допомогою гельхроматографії.…”

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Influence of Freeze-Thawing of Placenta on the Biological Activity of its Extracts

Bobrova¹,

Repina²,

Narozhnyi³

et al. 2019

Ukr. ž. med. bìol. sportu

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Low-Temperature Storage of Placenta Affects Anti-Inflammatory and Wound Healing Properties of Its Extracts

Bobrova¹,

Naumenko²,

Shchetinskyi³

et al. 2022

Probl Cryobiol Cryomed

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Here we have studied the effect of low-temperature storage of the placenta at different temperatures on anti-inflammatory activity of its extracts in cell models of erythrocyte thermohemolysis and adenosine-5’-diphosphate-induced platelet aggregation. The wound-healing effect of cryopreserved placental extracts was also evaluated in rat thermal burn model. When preserving placenta for up to a month, the temperature of storage was shown to cause no significant impact on the percentage of inhibition of erythrocyte thermohemolysis and platelet aggregation by the extracts. Placental storage for three months at –20°C decreased anti-inflammatory activity of its extracts, and led to its complete loss during 6 months. Placental cryopreservation at –196°C enabled to preserve the anti-inflammatory and wound-healing activity. The cryopreserved placenta-derived extracts showed a pronounced positive effect on reparative process at third-degree (B) skin burn injury in rats.

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Properties of aqueous-saline human placental extracts and their fractions after storage of placenta at various subzero temperatures

Cited by 4 publications

References 0 publications

Beneficial impact of human placenta extracts on erythrocyte membrane thermostability

Beneficial impact of human placenta extracts on erythrocyte membrane thermostability

Influence of Freeze-Thawing of Placenta on the Biological Activity of its Extracts

Low-Temperature Storage of Placenta Affects Anti-Inflammatory and Wound Healing Properties of Its Extracts

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