1977
DOI: 10.1021/bi00638a017
|View full text |Cite
|
Sign up to set email alerts
|

Properties of an altered RNA polymerase II activity from an α-amanitin-resistant mouse cell line

Abstract: alpha-Amanitin-resistant clones were selected in the mouse lymphoblastoid cell line L5178Y. One resistant clone, named A169b, was recloned and the properties of its DNA-dependent RNA polymerases were examined. The RNA polymerase II activity from A169b differs from the parental cell line in that approximately half the activity is resistant to 0.5 microgram/mL alpha-amanitin, while the parental enzyme is 50% inhibited at 0.005 microgram/mL. The enzymes from A169b and the parental line were purified free of polym… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

0
10
0

Year Published

1979
1979
2018
2018

Publication Types

Select...
3
2
1

Relationship

0
6

Authors

Journals

citations
Cited by 24 publications
(10 citation statements)
references
References 21 publications
0
10
0
Order By: Relevance
“…Surviving colonies were ex-VOL. 7,1987 588 BARTOLOMEI AND CORDEN panded, and their RNA polymerase activities were assayed (51) to determine the level of resistance. Cell lysates were assayed in the presence of increasing concentrations of a-amanitin, and the activity was expressed as a fraction of the counts incorporated when no amanitin was present.…”
Section: Resultsmentioning
confidence: 99%
See 3 more Smart Citations
“…Surviving colonies were ex-VOL. 7,1987 588 BARTOLOMEI AND CORDEN panded, and their RNA polymerase activities were assayed (51) to determine the level of resistance. Cell lysates were assayed in the presence of increasing concentrations of a-amanitin, and the activity was expressed as a fraction of the counts incorporated when no amanitin was present.…”
Section: Resultsmentioning
confidence: 99%
“…7,1987 on April 4, 2019 by guest http://mcb.asm.org/ 592 BARTOLOMEI AND CORDEN structure and function. First, the study of this mutation and other a-amanitin resistance mutations will elucidate the mechanism by which a-amanitin binds and inhibits RNA polymerase.…”
Section: Discussionmentioning
confidence: 99%
See 2 more Smart Citations
“…Alpha amanitin resistant variants have been isolated from Chinese hamster ovary cells (Chan et al, 1972;Ingles et al, 1976;Ingles, 1978;Lobban et al, 1976), murine lymphoblastoid cells (Bryant et al, 1977), baby hamster kidney (BHK) cells (Amati et al, 1975), and from rat L6 myoblasts (Somers et al, 1975;Crerar et al, 1977a,b). The basis for resistance has been related to an altered RNA polymcrasc I1 molecule.…”
Section: Discussionmentioning
confidence: 99%