Properties of amphibian renal arginase—I. The effect of dialysis and sulphydryl compounds

Characteristics of Arginase from the Terebellid Polychaete Pista Pacifica Berkeley

“…Glutathione has had a stimulatory effect on arginase on several occasions (Campbell, 1966). Glutathione, dithiothreitol, 2-mercaptoeth anol, and cysteine have a stimulatory effect on both bullfrog arginase and bovine arginase (Carlisky & Sadnik, 1972). The same effect was ob served with Pista.…”

“…The activity toward L-arginine is inhibited by p-chloromercuribenzoate in the cray fish (Hartenstein, 1971), chicken liver (Hora.!!. al., 1966), cockroach fat body (Reddy & Campbell, 1969a), and bullfrog kidney and bovine liver (Carlisky & Sadnik, 1972). Rat liver O'Iora et a!., 1966) and silkmoth are unaffected (Reddy & Campbell, 1969a).…”

“…This enzyme once thought to be present only in ureotelic species (Clementi, 1937) now appears to be widespread throughout the animal kingdom. Characterization data have been reported for an oligochaete annelid (Bishop & Campbell, 1965: Red dy & Campbell, 1968), a crayfish (Hartenstein, 1971), a mollusc (Camp bell, 1966), insects (Reddy & Campbell, 1969a), amphibians (Soberbn ~ al., 1967;Carlisky & Sadnik, 1972), reptiles and birds (Mora et al, 1965;Brown, 1966), and manunals (Greenberg, 1960;lIirsch-Kolb ~al. , 1970) • Several research groups have reported significant differences in arginases from ureotelic, uricotelic, and ammonotelic organisms with re spect to antigenic properties, molecular weights, Michaelis constants, substrate inhibition, and stability during dialysis (Mora et al, 1965(Mora et al, , 1966Soru, 1965;Brown, 1966;Soberon, et al, 1967;Reddy & Campbell, 1968, 1969aRossi & Grazi, 1969;Middlehoven, 1969;Hirsch-Kolb ~t al., 1970).…”

Structure and properties of arginase from the polychaete annelid Pista pacifica Berkeley

“…As illustrated by P. pacifica arginase, the effect of thiol groups on enzyme activity depends on whether the enzyme is incubated with the thiol-group inhibitor p-hydroxymercuribenzoate before the addition of arginine or whether the enzyme is added directly to a solution of arginine plus p-hydroxymercuribenzoate. When bullfrog and bovine arginases (Carlisky & Sadnik, 1972) are preincubated with GSH, dithiothreitol, 2-mercaptoethanol or cysteine, these thiolcontaining reagents have a stimulatory effect whereas p-chloromercuribenzoate has an inhibitory effect. P. pacifica arginase is unaffected by preincubation withp-hydroxymercuribenzoate.…”