Properties of adenylate cyclase and cyclic nucleotide phosphodiesterase in hamster isolated capillary preparations

Nemecek, Georgina M.

doi:10.1016/0304-4165(80)90359-1

Cited by 10 publications

(1 citation statement)

References 26 publications

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“…The use of endothelial cell cultures provides the advantage of providing a homogeneous cell population but has the disadvantage of low yield after several passages in culture [ 12 , 19 , 20 , 21 , 22 , 23 , 24 ]. It is necessary to obtain an appropriate amount of suspended, uncontaminated endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

Established Immortalized Cavernous Endothelial Cells Improve Erectile Dysfunction in Rats with Cavernous Nerve Injury

Bak¹,

Kim

et al. 2023

Pharmaceuticals

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The main cause of erectile dysfunction (ED) is the damage in penile cavernous endothelial cells (EC). Murine primary ECs have a limited growth potential, and the easy availability of murine ECs will facilitate the study of cavernous endothelial dysfunction in rats. This study was performed to establish immortalized rat penile cavernous ECs (rEC) and investigate how they could repair erectile dysfunction in rats with cavernous nerve injury (CNI). rEC was isolated enzymatically by collagenase digestion and were cultured. An amphotropic replication-incompetent retroviral vector encoding v-myc oncogene was used to transfect rEC for immortalization (vREC). Morphological and immunohistochemical properties of vREC were examined. Eight-week-old male Sprague-Dawley rats were divided into three groups of five rats each, including group 1 = sham operation, group 2 = bilateral CN injury, group 3 = vREC (1 × 106 cells) treatment after CNI. Erectile response was assessed at 2, 4 weeks after transplantation of vREC., Penile tissue were harvested at 4 weeks after transplantation and immune–histochemical examination was performed. vREC showed the expression of CD31, vWF, cell type-specific markers for EC by RT-PCR and flowcytometry. At 2, 4 weeks after transplantation, rats with CNI had significantly lower erectile function than control group (p < 0.05). The group transplanted with vREC showed higher erectile function than the group without vRECs (p < 0.05). vREC was established and repaired erectile dysfunction in rats with CNI. This cell line may be useful for studying mechanisms and drug screening of erectile dysfunction of rats.

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Section: Discussionmentioning

confidence: 99%

Established Immortalized Cavernous Endothelial Cells Improve Erectile Dysfunction in Rats with Cavernous Nerve Injury

Bak¹,

Kim

et al. 2023

Pharmaceuticals

View full text Add to dashboard Cite

show abstract

Regulation of endothelial cell function by cyclic nucleotides

Schafer¹,

Gimbrone²,

Handin³

1984

Developments in Cardiovascular Medicine

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Isolation of metabolically active endothelial cells in high yield from bovine cavernous bodies

1983

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Work on endothelial cells has been limited by the availability of procedures for obtaining such cells in quantities adequate for direct in vitro analysis. The present paper describes a method for the isolation of endothelial cells from bovine cavernous bodies. A number of cells ranging from 2.5 to 4 X 10(8) per animal has been obtained. The cells were identified as follows 1) presence of the "Weibel and Palade" bodies in the isolated cells, 2) "cobblestone" appearance of cell cultures, and 3) presence of factor VIII, as demonstrated by immunofluorescence assays. The cell viability at the end of the purification procedure was tested 1) by dye-exclusion tests and 2) by metabolic assays. Features of this preparation are 1) the very high yield of viable endothelial cells, 2) the absence of contamination by fibroblasts and smooth muscle cells and a very low contamination by erythrocytes and 3) the fine dispersion of the isolated cells. These properties allow functional and subcellular fractionation studies on freshly isolated endothelial cells of microvascular origin.

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Properties of adenylate cyclase and cyclic nucleotide phosphodiesterase in hamster isolated capillary preparations

Cited by 10 publications

References 26 publications

Established Immortalized Cavernous Endothelial Cells Improve Erectile Dysfunction in Rats with Cavernous Nerve Injury

Established Immortalized Cavernous Endothelial Cells Improve Erectile Dysfunction in Rats with Cavernous Nerve Injury

Regulation of endothelial cell function by cyclic nucleotides

Isolation of metabolically active endothelial cells in high yield from bovine cavernous bodies

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