1982
DOI: 10.1073/pnas.79.5.1578
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Properties of a Saccharomyces cerevisiae mtDNA segment conferring high-frequency yeast transformation.

Abstract: The bakers' yeast Saccharomyces cerevsiae is a facultative anaerobe, tolerant to mutations in its mitochondrial genome. Individual cytoplasmic petite mutants retain genetic information derived from any portion of the parental mtDNA, prompting questions concerning distribution of the DNA replication origin(s) on the yeast mitochondrial genome. The experiments described in this paper were designed to test the possibility of using high-frequency yeast transformation as a selection for yeast mtDNA sequences confer… Show more

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Cited by 80 publications
(31 citation statements)
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“…Nonetheless, a much more dramatic, 300-fold derepression occurred in cells carrying pLS7. The 10-fold-higher derepressed activity observed in strains carrying the multicopy episome pLS7 as compared with strains carrying a single integrated copy of pLS11 is consistent with the difference in copy number (16).…”
Section: Resultssupporting
confidence: 66%
“…Nonetheless, a much more dramatic, 300-fold derepression occurred in cells carrying pLS7. The 10-fold-higher derepressed activity observed in strains carrying the multicopy episome pLS7 as compared with strains carrying a single integrated copy of pLS11 is consistent with the difference in copy number (16).…”
Section: Resultssupporting
confidence: 66%
“…They transformed at high frequencies and were unstable, with the notable exception of pFL20 (see below). Their copy number varied between 15 and 80 as compared with 10 to 200 for different ars plasmids in S. cerevisiae (19,39). In both yeasts only a fraction of cells grown under selective conditions contain an ars plasmid (19,34,39).…”
Section: Discussionmentioning
confidence: 99%
“…Another general conclusion is that the present work provides precise examples of how physiological replication origins can be replaced by sequences which, though much less efficient, are still capable of insuring replication. It is very likely that sequences of the oril type also account for the replication as an extrachromosomal element in yeast of a hybrid plasmid containing a petite repeat unit devoid of any ori sequences (Hyman et al, 1982), but derived from a region rich in sequences of the orn family (de Zamaroczy et al, in preparation). As for the role of oril sequences as surrogate origins of replication, it should be stressed (Bernardi, 1982a) that the expansion (by replication slippage and unequal crossing-overs) of the duplicated and translocated ori sequences to form the intergenic sequences results in partial copies of ori sequence segments, still capable of interacting with the replication complex, being spread all over the genome.…”
Section: Discussionmentioning
confidence: 99%