1967
DOI: 10.1021/bi00857a005
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Properties of a Rat Tissue Iodothyronine Deiodinase and Its Natural Inhibitor*

Abstract: B-ring deiodination of L-thyroxine (T4) and L-triiodothyronine (Ta) by rat liver is enzymatic in nature, but the deiodinase activity in crude tissue homogenates is largely blocked by a naturally occurring inhibitor. The inhibitor is responsible for the time lag observed before deiodination takes place, and it is also responsible for the relative heat stability, pH dependence, and substrate inhibition of the system. All of these effects disappeared after the inhibitor was removed by dialysis. The specific activ… Show more

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Cited by 22 publications
(10 citation statements)
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“…The enzymatic nature of the deiodination of T4 and T3 by hepatic microsomes (12,27,28) and the increased rate of deiodination by microsomes from PB-treated rats have been demonstrated previously (20). Fig.…”
Section: Methodsmentioning
confidence: 53%
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“…The enzymatic nature of the deiodination of T4 and T3 by hepatic microsomes (12,27,28) and the increased rate of deiodination by microsomes from PB-treated rats have been demonstrated previously (20). Fig.…”
Section: Methodsmentioning
confidence: 53%
“…Furthermore, the increased rate of deiodination by PB-microsomes was accompanied by a proportional increase in the rate of microsomal NEI formation. Other workers have also reported the association of chromatographic origin radioactivity (presumably NEI) and iodide formation during the metabolism of the iodothyronines by liver homogenates (9,10,12) and microsomes (11). The lack of NEI formation during the metabolism of 3-monoiodotyrosine (MIT) suggests the specificity of this pathway for the iodothyronines.…”
Section: Discussionmentioning
confidence: 97%
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“…Rates of removal of the 5'-and 5-iodine of T4 are approximately equal in humans and rats (3,4), but these processes do not occur randomly. Decreased serum T3 concentrations, resulting from decreased peripheral 5'-deiodina-Many tissues, including liver, kidney, heart, skeletal muscle, leukocytes, and fibroblasts, are capable of deiodinating T4 in vitro (24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39). The techniques used to demonstrate T4 deiodination in these studies included incubation of tissue homogenates, tissue slices, dispersed cells, and whole organ perfusion.…”
mentioning
confidence: 99%