Properties of a Proteus morganii Bacteriocin

Smit, J. A.; Klerk, H. C. De; Coetzee, J. N.

doi:10.1099/00221287-54-1-67

Cited by 11 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bactericidal effect of Bac R. on a group A streptococcal strain. The bactericidal effect of Bac R1 against Streptococcus pyogenes strain M57 was monitored during growth of the streptococci in HI broth containing 20 AU of bacteriocin per ml. As a control, phosphate buffer was added to an identically treated culture.…”

Section: Resultsmentioning

confidence: 99%

“…Staphylococcins can be sensitive to mechanical agitation (11), but 30 min of continuous high-speed vortexing failed to reduce the activity of a Bac R. solution. Other bacteriocins are inactivated by oxidation (20), but Bac R. was not inactivated after incubation at 370C in a final concentration of 10 mM H202 for 2 h. Several enzymes were then interacted with Bac R1 to determine whether they affected bactericidal activity. Lysostaphin did not reduce Bac R. activity.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Production and properties of a staphylococcin genetically controlled by the staphylococcal plasmid for exfoliative toxin synthesis

Rogolsky¹,

Wiley²

1977

Infect Immun

View full text Add to dashboard Cite

Previous data from this laboratory showed that certain phage group 2 staphylococci contain a large 568 virulence plasmid containing genes that code for both exfoliative toxin (ET) and a specific staphylococcin. Optimal cultural conditions for bacteriocin production were similar to those found for ET production. The bacteriocin is an extracellular product produced in small quantities that can be neither extracted from cell pellets with 1 M NaCl nor induced with mitomycin C. The staphylococcin is active against a wide variety of gram-positive organisms and also against group 2 staphylococcal strains that have been cured of the plasmid carrying the staphylococcin marker. The bacteriocin is not inactivated by oxidation, mechanical agitation, or boiling for 15 min. It is sensitive to the action of trypsin and Pronase but not lysostaphin and is stable within a pH range of 4 to 9. It has an isoelectric point of approximately 7.7. Removal of the ampholytes and glycerol from electrofocused staphylococcin preparations resulted in total loss of bacteriocin activity.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Production and properties of a staphylococcin genetically controlled by the staphylococcal plasmid for exfoliative toxin synthesis

Rogolsky¹,

Wiley²

1977

Infect Immun

View full text Add to dashboard Cite

show abstract

“…Although the action of acnecin seems to be different in this respect, it has a notable feature in common with other bacteriocins. As Jetten and Vogels pointed out (14), there are many similarities in amino acid composition among bacteriocins from different species of S. aureus (14), S. marcescens (8), Lactobacillus fermenti (5), and Proteus morganii (30) and colicin (11). The most striking similarity is high content of aspartic acid, glutamic acid, glycine, and alanine.…”

Section: Methodsmentioning

confidence: 99%

Purification and Properties of a Bacteriocin-Like Substance (Acnecin) of Oral Propionibacterium acnes

Fujimura

Nakamura

1978

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Propionibacterium acnes CN-8, isolated from human dental plaque, was grown in a liquid medium, and its bacteriocin-like substance (acnecin) was extracted from the cells by ultrasonic treatment. Acnecin was purified to a homogeneous state with recovery of 47%. Specific activity increased 72-fold in comparison with the crude extract. The properties of acnecin were as follows. (i) Acnecin may consist of five subunits with a molecular weight of about 12,000. (ii) Its isoelectric point was 5.5. (iii) In amino acid composition, aspartic acid, glutamic acid, glycine, and alanine were predominant, whereas cystine was not present. (iv) Acnecin contained 3.3% carbohydrate but was substantially free from lipid. (v) The activity was lost by heating at 60°C or by protease and lysozyme treatments. Acnecin acted bacteriostatically on the indicator strain without killing it. The action spectrum of acnecin was very narrow; it was effective only against strains of nonacnecin-producing P. acnes and Corynebacterium parvum, a species closely related to P. acnes.

show abstract

“…The protracted accumulation of morganocin in Proteus cultures reflected its stability in the media, in contrast to the bacteriocin produced by P. morganii MR336, which had to be stabilized by addition of sodium sulfite (27). A long lag period after induction (maximum production 36 to 48 h after induction) has also been described for streptococcin A (28).…”

Section: Resultsmentioning

confidence: 99%

“…Many are coded for by plasmids which have been identified by isolation (25), elimination (16,27), or mobilization as a result of their sex factor activity (22) or that of a mobilizing plasmid (29). Several bacteriocins have been purified and characterized to varying degrees, and their mode of action has been established (see 9).…”

mentioning

confidence: 99%

Mobilization of Morganocin 174 Plasmid and Kinetics of Morganocin Production in Proteus and Escherichia coli Hosts

Williams

1977

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Morganocin 174 is coded by a plasmid, Mor174. The plasmid is not self-transmissible but may be mobilized by resistance factor R772. Morganocin synthesis in all Proteus and Providencia strains carrying Mor174 was characterized by a longer lag period after induction and higher titers than in Escherichia coli B(Mor174). The low titers obtained in E. coli B(Mor174) are due to a heatstable inhibitor produced by this strain. Synthesis of morganocin is not constitutive and may be induced by ultraviolet irradiation or mitomycin C. Morganocin production is not influenced by the growth medium of the organism.

show abstract

Properties of a Proteus morganii Bacteriocin

Cited by 11 publications

References 20 publications

Production and properties of a staphylococcin genetically controlled by the staphylococcal plasmid for exfoliative toxin synthesis

Production and properties of a staphylococcin genetically controlled by the staphylococcal plasmid for exfoliative toxin synthesis

Purification and Properties of a Bacteriocin-Like Substance (Acnecin) of Oral Propionibacterium acnes

Mobilization of Morganocin 174 Plasmid and Kinetics of Morganocin Production in Proteus and Escherichia coli Hosts

Contact Info

Product

Resources

About