Properties of a polarized primary culture from rat renal inner medullary collecting duct (IMCD) cells

Ruhfus, Birgit; Bauernschmitt, H. G.; Kinne, Rolf

doi:10.1007/s11626-998-0128-4

Cited by 20 publications

(17 citation statements)

References 22 publications

(10 reference statements)

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“…In addition, these cells form tight monolayers with high transepithelial resistance (ϳ1350 ohms ϫ cm 2 ). This high electrical resistance feature was recently shown also to be exhibited in primary cultures of rat IMCD cells, where resistance exceeded 1000 ohms ϫ cm 2 (25). mIMCD-3 cells were grown in a mixture of Dulbecco's modified Eagle's medium and Ham's F-12 (equal parts) supplemented with 10% fetal bovine serum, 100 units/ml penicillin, and 100 g/ml of streptomycin at 37°C in 95% air and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 90%

Bradykinin B2 Receptors Activate Na+/H+ Exchange in mIMCD-3 Cells via Janus Kinase 2 and Ca2+/Calmodulin

Mukhin¹,

Vlasova²,

Jaffa

et al. 2001

Journal of Biological Chemistry

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We used a cultured murine cell model of the inner medullary collecting duct (mIMCD-3 cells) to examine the regulation of the ubiquitous sodium-proton exchanger, Na ؉ /H ؉ exchanger isoform 1 (NHE-1), by a prototypical G protein-coupled receptor, the bradykinin B 2 receptor. Bradykinin rapidly activates NHE-1 in a concentration-dependent manner as assessed by proton microphysiometry of quiescent cells and by 2-7-bis[2-carboxymethyl]-5(6)-carboxyfluorescein fluorescence measuring the accelerated rate of pH i recovery from an imposed acid load. The activation of NHE-1 is blocked by inhibitors of the bradykinin B 2 receptor, phospholipase C, Ca 2؉ /calmodulin (CaM), and Janus kinase 2 (Jak2), but not by pertussis toxin or by inhibitors of protein kinase C and phosphatidylinositol 3-kinase. Immunoprecipitation studies showed that bradykinin stimulates the assembly of a signal transduction complex that includes CaM, Jak2, and NHE-1. CaM appears to be a direct substrate for phosphorylation by Jak2 as measured by an in vitro kinase assay. We propose that Jak2 is a new indirect regulator of NHE-1 activity, which modulates the activity of NHE-1 by increasing the tyrosine phosphorylation of CaM and most likely by increasing the binding of CaM to NHE-1.

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Section: Methodsmentioning

confidence: 90%

Bradykinin B2 Receptors Activate Na+/H+ Exchange in mIMCD-3 Cells via Janus Kinase 2 and Ca2+/Calmodulin

Mukhin¹,

Vlasova²,

Jaffa

et al. 2001

Journal of Biological Chemistry

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“…The hypoosmotic activation of sorbitol and betaine efflux from these cells is dependent upon G-proteins whereas that of taurine, myo-inositol and glycerophosphorylcholine is not significantly so [44]. Furthermore, when cells are grown in confluent layers, the extent to which the different organic osmolytes are released from one or other side of the polarised cell layer varies [16,45]. These observations are consistent with the operation of multiple hypoosmotically-activated organic osmolyte pathways, with varying substrate specificity.…”

Section: CLmentioning

confidence: 99%

Organic Osmolyte Channels: A Comparative View

Junankar

Kirk²

2000

Cell Physiol Biochem

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Cells respond to osmotic swelling by releasing inorganic ions and small organic molecules (organic osmolytes). In many cell-types, osmotic swelling results in the activation of an outwardly-rectifying anion-selective current. The channel underlying this current has a significant permeability to a number of organic osmolytes and may play a role in the hypoosmotically-activated efflux of these compounds. However, there is also evidence that the volume-regulatory efflux of organic osmolytes involves other pathways which may be selective for neutral osmolytes over anions.

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“…In the former cellular polarity is well maintained in the latter a high degree of differentiation is achieved [30].…”

Section: Imcd Cells Contain a Calcium Regulated Sorbitol Channelmentioning

confidence: 99%

Regulation of Sorbitol Efflux in Different Renal Medullary Cells: Similarities and Diversities

Kinne

Tinel²,

Kipp³

et al. 2000

Cell Physiol Biochem

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It is well accepted that organic osmolytes, including sorbitol, play a major role in the volume regulation of renal medullary cells. The signal leading to an activation of release channels during RVD is, however, poorly understood. Hypotonicity induced sorbitol efflux was investigated in freshly isolated rat inner medullary collecting duct (IMCD) cells and in rabbit medullary thick ascending limb of Henle’s loop (TALH) cells biochemically or using labeled sorbitol. The time course of release was compared with changes in cell volume, measured by confocal microscopy, and alterations in cell calcium (Ca_i) determined by Fura 2 technology. In IMCD cells sorbitol release, volume decrease and Ca_i transients show a close temporal correlation. In addition increases in Ca_i without volume changes stimulate sorbitol efflux. In TALH cells sorbitol release starts after a significant lag time and reaches a maximum when cell volume is already partially restored. The same discrepancy is observed with regard to changes in Ca_i and sorbitol efflux. These studies suggest that in IMCD cells changes in Ca_i are the main regulator for the sorbitol permeability of the plasma membrane. The sorbitol channel present in TALH cells seems to operate predominantly independently of Ca_i. Despite this diversity in signal transduction the sorbitol channels in both renal cell types appear, however, not to be stretch-activated.

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Properties of a polarized primary culture from rat renal inner medullary collecting duct (IMCD) cells

Cited by 20 publications

References 22 publications

Bradykinin B2 Receptors Activate Na+/H+ Exchange in mIMCD-3 Cells via Janus Kinase 2 and Ca2+/Calmodulin

Bradykinin B2 Receptors Activate Na+/H+ Exchange in mIMCD-3 Cells via Janus Kinase 2 and Ca2+/Calmodulin

Organic Osmolyte Channels: A Comparative View

Regulation of Sorbitol Efflux in Different Renal Medullary Cells: Similarities and Diversities

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