Properties of a Fatty Acid Forming Organism Isolated From the Rumen of Sheep

Elsden, S. R.; Volcani, Benjamin E.; Gilchrist, F. M. C.; Lewis, D.

doi:10.1128/jb.72.5.681-689.1956

Cited by 150 publications

(74 citation statements)

References 22 publications

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“…The level of JCM at the cecum decreased to the 10 8 /pouch (10 7 /g digesta)‐level in that study but still worked efficiently. JCM is of ovine rumen origin (Elsden et al . 1956); therefore, this strain had not been checked to determine if it is resistant to gastric LAB and gastro‐intestinal digestion.…”

Section: Discussionmentioning

confidence: 99%

Stimulation of butyrate production through the metabolic interaction among lactic acid bacteria, Lactobacillus acidophilus, and lactic acid‐utilizing bacteria, Megasphaera elsdenii, in porcine cecal digesta

Tsukahara

Hashizume

Koyama

et al. 2006

Animal Science Journal

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In this study, we examined the potential of Megasphaera elsdenii as a probiotic agent in combination with sodium gluconate. We also examined the combination of M. elsdenii with probiotic lactic acid bacteria (LAB), Lactobacillus acidophilus, to enhance butyrate production in the large intestine using a pig cecal in vitro model under the presence of sodium gluconate. Compared to the uninoculated control culture (with only diluted cecal digesta), the addition of M. elsdenii and LAB to the culture stimulated significantly the production of n-butyrate (60% increase) and n-valerate (50% increase) after 24 h incubation. n-Butyrate is regarded as the most important short-chain fatty acid in the large intestine because it stimulates the proliferation of epithelial cells, mucus release and water and mineral absorption. Therefore, lactate-utilizing butyrate producers such as M. elsdenii play a significant role in supporting the health-beneficial effects of lactogenic prebiotics and probiotics LAB. The potential of M. elsdenii as a probiotic was also suggested.

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Section: Discussionmentioning

confidence: 99%

Stimulation of butyrate production through the metabolic interaction among lactic acid bacteria, Lactobacillus acidophilus, and lactic acid‐utilizing bacteria, Megasphaera elsdenii, in porcine cecal digesta

Tsukahara

Hashizume

Koyama

et al. 2006

Animal Science Journal

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“…The reactor was purged with high-purity (99.999%) nitrogen gas to guarantee anaerobic conditions after it was sealed. The basic culture medium per liter contained NaHCO 3 , 5,040 mg; NH 4 12 , 0.001 mg; and lipoic acid, 0.05 mg. All the chemicals used in the study were of analytical grade or better.…”

Section: Methodsmentioning

confidence: 99%

“…E. pyruvativorans has been reported to harbor metabolic properties analogous to those of C. kluyveri (10). Additionally, n-caproic acid can be produced from lactate by a ruminal Ruminococcaceae bacterium (11) and Megasphaera elsdenii (12).…”

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confidence: 99%

Metabolic Interactions of a Chain Elongation Microbiome

Han

Shao

et al. 2018

Appl Environ Microbiol

107

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Carbon chain elongation (CCE), a reaction within the carboxylate platform that elongates short-chain to medium-chain carboxylates by mixed culture, has attracted worldwide interest. The present study provides insights into the microbial diversity and predictive microbial metabolic pathways of a mixed-culture CCE microbiome on the basis of a comparative analysis of the metagenome and metatranscriptome. We found that the microbial structure of an acclimated chain elongation microbiome was a highly similar to that of the original inoculating biogas reactor culture; however, the metabolic activities were completely different, demonstrating the high stability of the microbial structure and flexibility of its functions. Additionally, the fatty acid biosynthesis (FAB) pathway, rather than the well-known reverse β-oxidation (RBO) pathway for CCE, was more active and pivotal, though the FAB pathway had more steps and consumed more ATP, a phenomenon that has rarely been observed in previous CCE studies. A total of 91 draft genomes were reconstructed from the metagenomic reads, of which three were near completion (completeness, >97%) and were assigned to unknown strains of ,, and The last two strains are likely new-found active participators of CCE in the mixed culture. Finally, a conceptual framework of CCE, including both pathways and the potential participators, was proposed. Carbon chain elongation means the conversion of short-chain volatile fatty acids to medium-chain carboxylates, such as -caproate and-caprylate with electron donors under anaerobic condition. This bio-reaction can both expand the resource of valuable biochemicals and broaden the utilization of low-grade organic residues in a sustainable biorefinery context. is conventionally considered model microbe for carbon chain elongation which uses the reverse β-oxidation pathway. However, little is known about the detailed microbial structure and function of other abundant microorganism in a mixed culture (or open culture) of chain elongation. We conducted the comparative metagenomic and metatranscriptomic analysis of a chain elongation microbiome to throw light on the underlying functional microbes and alternative pathways.

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“…In a review article, Oxford (1958) (1952) concluded that Clostridium pro-plonlcum appears to form propionate from lactate by a more direct mechanism without production of intermediary dlcarboxylic acids and without Involving carbon dioxide fixation followed by subsequent decarboxylation. Elsden et al (1956) isolated an unidentified organism from the rumen of sheep that fermented lactate directly to propionate. Ladd (19 59) con firmed Elsden's observations.…”

mentioning

confidence: 99%