Properties of a bacterium which degrades solid poly(tetramethylene succinate)-co-adipate, a biodegradable plastic

Uchida, Hiromi; Nakajima-Kambe, Toshiaki; Shigeno-Akutsu, Yukie; Nomura, Nobuhiko; Tokiwa, Yutaka; Nakahara, Tadaatsu

doi:10.1111/j.1574-6968.2000.tb09201.x

Cited by 78 publications

(31 citation statements)

References 9 publications

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“…Unlike other petrochemical-based resins that take centuries to degrade after disposal, these polymers break down rapidly into carbon dioxide, water, and humus under appropriate conditions when they are exposed to the combined attack of water and micro-organisms. [2,3] These products also meet advanced composting standards and typically break down in twelve weeks under aerobic conditions. [4] In this direction, poly[(butylene succinate)-co-adipate] (PBSA) is among the most promising polymer.…”

Section: Introductionmentioning

confidence: 99%

Crystallization Behavior of Poly[(butylene succinate)‐co‐adipate] Nanocomposite

Ray

Bousmina

2006

Macro Chemistry & Physics

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Summary: The unique crystallization behavior of a poly[(butylene succinate)‐co‐adipate] (PBSA) nanocomposite is addressed. Nanocomposites have been prepared by melt blending PBSA and organically modified synthetic fluorine mica (OSFM) in a batch mixer. The structure of the nanocomposite is studied by using X‐ray diffraction and transmission electron microscopy, which reveal a coexistence of exfoliated and intercalated silicate layers homogeneously dispersed in the PBSA matrix. The non‐isothermal crystallization behavior of PBSA and the nanocomposite samples is studied by differential scanning calorimetry (DSC). Various models, namely the Avrami method, the Ozawa method, and the combined Avrami‐Ozawa method, are applied to describe the kinetics of the non‐isothermal crystallization. All analyses reveal that the incorporation of the OSFM alters the crystallization properties of PBSA but in ways unexpected from other polymer nanocomposite systems. Polarized optical microscopy is used to support this conclusion. The activation energy for the non‐isothermal crystallization of both samples is evaluated by using three different methods. The results show that the absolute value of the activation energy for the nanocomposite is higher than that of the neat polymer. This indicates the slower crystallization kinetics of the nanocomposite. The effect of incorporation of OSFM on the cold crystallization behavior of neat PBSA is also studied by both conventional and temperature‐modulated DSC.Polarized optical microscopy image of the PBSA/OSFM nanocomposite at 70 °C during non‐isothermal crystallization from the melt.magnified imagePolarized optical microscopy image of the PBSA/OSFM nanocomposite at 70 °C during non‐isothermal crystallization from the melt.

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Section: Introductionmentioning

confidence: 99%

Crystallization Behavior of Poly[(butylene succinate)‐co‐adipate] Nanocomposite

Ray

Bousmina

2006

Macro Chemistry & Physics

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“…PBSA is biologically degradable when present in composts, moist soil, fresh water with activated sludge, and seawater (1,12). Various microorganisms capable of PBSA degradation have been isolated (27,30).…”

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confidence: 99%

Novel Hydrophobic Surface Binding Protein, HsbA, Produced by Aspergillus oryzae

Ohtaki

Maéda

Takahashi

et al. 2006

Appl Environ Microbiol

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Hydrophobic surface binding protein A (HsbA) is a secreted protein (14.5 kDa) isolated from the culture broth of Aspergillus oryzae RIB40 grown in a medium containing polybutylene succinate-co-adipate (PBSA) as a sole carbon source. We purified HsbA from the culture broth and determined its N-terminal amino acid sequence. We found a DNA sequence encoding a protein whose N terminus matched that of purified HsbA in the A. ozyzae genomic sequence. We cloned the hsbA genomic DNA and cDNA from A. oryzae and constructed a recombinant A. oryzae strain highly expressing hsbA. Orthologues of HsbA were present in animal pathogenic and entomopathogenic fungi. Heterologously synthesized HsbA was purified and biochemically characterized. Although the HsbA amino acid sequence suggests that HsbA may be hydrophilic, HsbA adsorbed to hydrophobic PBSA surfaces in the presence of NaCl or CaCl 2 . When HsbA was adsorbed on the hydrophobic PBSA surfaces, it promoted PBSA degradation via the CutL1 polyesterase. CutL1 interacts directly with HsbA attached to the hydrophobic QCM electrode surface. These results suggest that when HsbA is adsorbed onto the PBSA surface, it recruits CutL1, and that when CutL1 is accumulated on the PBSA surface, it stimulates PBSA degradation. We previously reported that when the A. oryzae hydrophobin RolA is bound to PBSA surfaces, it too specifically recruits CutL1. Since HsbA is not a hydrophobin, A. oryzae may use several types of proteins to recruit lytic enzymes to the surface of hydrophobic solid materials and promote their degradation.

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“…11,12 The compost sample (10 g) and distilled water (100 mL) were mixed for 10 min, then the suspension solution was allowed to settle down for 30 min. The supernatant was pipetted (0.5 mL) and inoculated to enrichment medium (10 mL).…”

Section: -13mentioning

confidence: 99%

Poly(L‐lactide)‐degrading activity of a newly isolated bacterium

Kim

Weon

et al. 2008

J of Applied Polymer Sci

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A new strain exhibiting poly(L-lactic acid) (PLLA)-degrading activity was isolated from the compost loaded with low-molecular weight PLLA. The strain was rod-shaped gram positive bacterium and was identified to be Bacillus licheniformis by the 16S rDNA sequence analysis. It degraded not only low-molecular weight PLLA but also other PLLAs having higher molecular weight. The lower the molecular weight of PLLA, the faster the biodegradation rate. And thereby 45% and 81% of PLLAs with weight average molecular weight of 256,000 and 5,000, respectively, were metabolized into CO 2 after 40 days of biodegradation at 588C in the sterilized compost inoculated with the bacterium. According to the 16S rDNA sequence analysis, B. licheniformis PLLA-2 belonged to Bacillus spp. phylogenetically. However, there existed almost no phylogenetic relationship between B. licheniformis PLLA-2 and the previously reported PLLA-degrading strains.

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Properties of a bacterium which degrades solid poly(tetramethylene succinate)-co-adipate, a biodegradable plastic

Cited by 78 publications

References 9 publications

Crystallization Behavior of Poly[(butylene succinate)‐co‐adipate] Nanocomposite

Crystallization Behavior of Poly[(butylene succinate)‐co‐adipate] Nanocomposite

Novel Hydrophobic Surface Binding Protein, HsbA, Produced by Aspergillus oryzae

Poly(L‐lactide)‐degrading activity of a newly isolated bacterium

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