Properties and the locations of a set of fluorescent probes sensitive to the fluidity gradient of the lipid bilayer

Thulborn, Keith R.; Sawyer, William H.

doi:10.1016/0005-2736(78)90308-5

Cited by 239 publications

(108 citation statements)

References 23 publications

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“…These effects in rate and magnitude are likely due to the smaller changes in microviscosity which occur near the glycerol head region of phosphatidylcholine relative to the center of the bilayer as the temperature is increased through the phase-transition region. Similar f5nlings were reported in [9]. The apolipoproteins produced a change in polarization amplitude (AP) in the liquid crystalline (1) and gel-crystalline (g) states of the lipid.…”

Section: Resultssupporting

confidence: 76%

Effects of human plasma apolipoproteins C‐I, C‐II and C‐III on the phase‐transition of sonicated vesicles of dipalmitoylphosphatidylcholine

Cardin¹,

Jackson²,

Johnson³

1982

FEBS Letters

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Section: Resultssupporting

confidence: 76%

Effects of human plasma apolipoproteins C‐I, C‐II and C‐III on the phase‐transition of sonicated vesicles of dipalmitoylphosphatidylcholine

Cardin¹,

Jackson²,

Johnson³

1982

FEBS Letters

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“…The static component of membrane fluidity can be assessed by probes such as DPH, while the dynamic component can be assessed with probes that undergo primarily rotational movements within the bilayer such as the anthroyloxy-labeled fatty acids ( 18). Inasmuch as the latter probes can have the fluorescent anthroyloxy group linked to any carbon of the base fatty acid, this property ofthe membrane can be evaluated as a function of depth within the bilayer (19,20)., Table II presents data obtained with the probe DPH. Microvillus membrane obtained from crypt cells appeared significantly more fluid than that found in either villus-tip or midvillus cells.…”

Section: Resultsmentioning

confidence: 99%

Glucose transport and microvillus membrane physical properties along the crypt-villus axis of the rabbit.

Meddings¹,

Souza²,

Goel³

et al. 1990

J. Clin. Invest.

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Both transport function and microvillus membrane physical properties evolve as the enterocyte matures and migrates up the crypt-villus axis. We isolated enriched fractions of villus tip, mid-villus, and crypt enterocytes from which microvillus membrane vesicles were prepared. Using this material we characterized the alterations that occur in microvillus membrane fluidity as the rabbit enterocyte matures and correlated these with kinetic studies of glucose transport. With increasing maturity the microvillus membrane becomes more rigid due to both an increase in the cholesterol/phospholipid ratio and alterations in individual phospholipid subclasses. Maximal rates of glucose transport were greatest in microvillus membrane vesicles prepared from mature cells. However, the glucose concentration producing half-maximal rates of transport (K.) was significantly lower in crypt microvillus membrane vesicles, suggesting that a distinct glucose transporter existed in crypt enterocytes. This distinction disappeared when differences between membrane lipid environments were removed. By fluidizing villus-tip microvillus membrane vesicles, in vitro, to levels seen in the crypt microvillus membrane, we observed a reduction in the K. of this transport system. These data suggest that the kinetic characteristics of the sodium-dependent glucose transporter are dependent upon its local membrane environment. (J. Clin. Invest. 1990Invest. . 85:1099Invest. -1107.) membrane fluidity -nutrient absorption -transport kinetics

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“…The photophysical features of these probes and their application to the study of vesicles and membranes were intensively studied in the past. [36][37][38][39] All the fluorescence anisotropy measurements were made using a Perkin Elmer LS 55B fluorescence spectrophotometer, fitted with temperature control and magnetic stirrer. Temperature was measured with an accurance of 0.1 ºC, and the stirrer switched off during the experiments.…”

Section: Fluorescence Anisotropymentioning

confidence: 99%

Interaction of arginine-based cationic surfactants with membranes. An experimental and molecular simulation study

Almeida¹,

Morán²,

Infante³

et al. 2009

Arkivoc

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This work is dedicated to Prof. António M. d'A. Rocha Gonçalves, on the occasion of his 70 th birthday: to the teacher, scientist and academic AbstractThe effect of two arginine-based cationic surfactants, arginine N-lauroyl amide dihydrochloride and arginine O-lauroyl ester dihydrochloride, upon dipalmitoylphosphatidylcholine liposomes, is addressed in this work. Some aspects concerning the synthesis of these surfactants are also presented in some detail. Differential scanning calorimetry, as well as fluorescence anisotropy using several probes, is used to assess the relative effects on the lipid model membranes. In spite of the structural similarity among these molecules, which differ only by the group that connects the polar head to the tail, experimental results suggest differences in their behavior. Molecular dynamics simulation provides significant insight into the molecular mechanism that governs this interaction. A rationale is provided in terms of lipid-surfactant hydrogen bonding and the consequent positioning of the surfactant molecule within the bilayer.

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Properties and the locations of a set of fluorescent probes sensitive to the fluidity gradient of the lipid bilayer

Cited by 239 publications

References 23 publications

Effects of human plasma apolipoproteins C‐I, C‐II and C‐III on the phase‐transition of sonicated vesicles of dipalmitoylphosphatidylcholine

Effects of human plasma apolipoproteins C‐I, C‐II and C‐III on the phase‐transition of sonicated vesicles of dipalmitoylphosphatidylcholine

Glucose transport and microvillus membrane physical properties along the crypt-villus axis of the rabbit.

Interaction of arginine-based cationic surfactants with membranes. An experimental and molecular simulation study

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