Properties and Regulation of Glutathione Peroxidase

Little, C. H. A.; Olinescu, R; Reid, Kristina; O’Brien, Peter J.

doi:10.1016/s0021-9258(18)62973-4

Cited by 234 publications

(8 citation statements)

References 24 publications

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“…To determine the inhibition of GPx, an established microplate reader-based assay was performed with minor modifications. , For this purpose, commercially available fetal bovine erythrocytes GPx (Sigma-Aldrich) were used and diluted with distilled water to achieve a concentration of 100 ng/mL. The compounds were freshly dissolved as stock solutions in DMF.…”

Section: Methodsmentioning

confidence: 99%

Comparative in Vitro Evaluation of N-Heterocyclic Carbene Gold(I) Complexes of the Benzimidazolylidene Type

et al. 2011

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Gold(I) complexes with a 1,3-diethylbenzimidazol-2-ylidene N-heterocyclic carbene (NHC) ligand of the type NHC-Au-L (L=-Cl, -NHC, or -PPh3) were comparatively evaluated as thioredoxin reductase (TrxR) inhibitors and antimitochondrial anticancer agents. Different effects were noted in various biochemical assays (e.g., inhibition of TrxR, cellular and mitochondrial uptake, or effects on mitochondrial membrane potential), and this was related to properties of the complexes such as bond dissociation energies and overall charge. Remarkable antiproliferative effects, a strong induction of apoptosis, and enhancement of reactive oxygen species (ROS) formation as well as other effects on tumor cell metabolism confirmed the promising potential of the complexes as novel anticancer chemotherapeutics.

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Section: Methodsmentioning

confidence: 99%

Comparative in Vitro Evaluation of N-Heterocyclic Carbene Gold(I) Complexes of the Benzimidazolylidene Type

et al. 2011

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“…20 With the inclusion of glutathione peroxidase (GPX, 10 unit cm Ϫ3 ) and small amounts of reduced glutathione (GSH, 0.3 mmol dm Ϫ3 ) in the reaction mixture the reduction of LAMPPN-OOH to LAMPPN-OH was expected. 31 However, when we added GPX and GSH to the reaction mixture the LAMPPN-OOH signal was replaced by a 50 min persistent broad signal [a P = 4.20 mT, a N = 1.45 mT, a H = 0.34 mT, Fig. 1(f)] characteristic of the decomposition of the LAMPPN-OH spin adduct (results not shown).…”

Section: Superoxide Spin-trapping Studies In Phosphate Buffermentioning

confidence: 96%

Synthesis and spin-trapping behaviour of glycosylated nitrones

Ouari¹,

Chalier²,

Bonaly³

et al. 1998

J. Chem. Soc., Perkin Trans. 2

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“…Reactions were started by the addition of 1 mM GSSG and followed spectrophotometrically at 340 nm. Glutathione peroxidase activity (0.5 mg proteins) was estimated at 25 °C in 50 mM Hepes/Tris ( pH 7.0) and EDTA 3 mM, 0.3 mM NADPH, 5 mM GSH and 0.25 mM tert-butyl hydroperoxide according to Little et al 70…”

Section: Enzyme Inhibitionmentioning

confidence: 99%

Synthesis and characterization of azolate gold(i) phosphane complexes as thioredoxin reductase inhibiting antitumor agents

et al. 2012

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Following an increasing interest in the gold drug therapy field, nine new neutral azolate gold(I) phosphane compounds have been synthesized and tested as anticancer agents. The azolate ligands used in this study are pyrazolates and imidazolates substituted with deactivating groups such as trifluoromethyl, nitro or chloride moieties, whereas the phosphane co-ligand is the triphenylphosphane or the more hydrophilic TPA (TPA = 1,3,5-triazaphosphaadamantane). The studied gold(I) complexes are: (3,5-bis-trifluoromethyl-1H-pyrazolate-1-yl)-triphenylphosphane-gold(I) (1), (3,5-dinitro-1H-pyrazolate-1-yl)-triphenylphosphane-gold(I) (2), (4-nitro-1H-pyrazolate-1-yl)-triphenylphosphane-gold(I) (5), (4,5-dichloro-1H-imidazolate-1-yl)-triphenylphosphane-gold(I) (7), with the related TPA complexes (3), (4), (6) and (8) and (1-benzyl-4,5-di-chloro-2H-imidazolate-2-yl)-triphenylphosphane-gold(I) (9). The presence of deactivating groups on the azole rings improves the solubility of these complexes in polar media. Compounds 1-8 contain the N-Au-P environment, whilst compound 9 is the only one to contain a C-Au-P environment. Crystal structures for compounds 1 and 2 have been obtained and discussed. Interestingly, the newly synthesized gold(I) compounds were found to possess a pronounced cytotoxic activity on several human cancer cells, some of which were endowed with cis-platin or multidrug resistance. In particular, among azolate gold(I) complexes, 1 and 2 proved to be the most promising derivatives eliciting an antiproliferative effect up to 70 times higher than cis-platin. Mechanistic experiments indicated that the inhibition of thioredoxin reductase (TrxR) might be involved in the pharmacodynamic behavior of these gold species.

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Properties and Regulation of Glutathione Peroxidase

Cited by 234 publications

References 24 publications

Comparative in Vitro Evaluation of N-Heterocyclic Carbene Gold(I) Complexes of the Benzimidazolylidene Type

Comparative in Vitro Evaluation of N-Heterocyclic Carbene Gold(I) Complexes of the Benzimidazolylidene Type

Synthesis and spin-trapping behaviour of glycosylated nitrones

Synthesis and characterization of azolate gold(i) phosphane complexes as thioredoxin reductase inhibiting antitumor agents

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