Properties and in vitro characterization of polyhydroxybutyrate–chitosan scaffolds prepared by modified precipitation method

Medvecký, Ľubomír; Giretová, Maria; Štulajterová, Radoslava

doi:10.1007/s10856-013-5105-0

Cited by 30 publications

(24 citation statements)

References 52 publications

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“…Increasing the wavenumber of the CO group is a result of disturbances in the crystalline phase of PHB after addition of CTS . This result is in agreement with the literature . Also, peaks at about 973 cm −1 and 1293 cm −1 also shifted to higher wavenumbers.…”

Section: Resultssupporting

confidence: 92%

“…also reported that CTS enhanced cell adhesion and proliferation on PLGA/CTS blend scaffolds . In a similar study on PHB/CTS blend scaffolds fabricated by co‐precipitation method, it was shown that CTS could increase the initial attachment of fibroblast cells …”

Section: Resultsmentioning

confidence: 77%

“…40 This result is in agreement with the literature. 41 Also, peaks at about 973 cm 21 and 1293 cm 21 also shifted to higher wavenumbers. Presence and weakening of these peaks as a result of the addition of CTS are attributed to a decrease in crystallinity of PHB.…”

Section: Sem Investigationsmentioning

confidence: 91%

See 2 more Smart Citations

Electrospun poly(hydroxybutyrate)/chitosan blend fibrous scaffolds for cartilage tissue engineering

Sadeghi

Karbasi

Razavi

et al. 2016

J of Applied Polymer Sci

113

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In this study, polyhydroxybutyrate (PHB) was blended with chitosan (CTS), and electrospun in order to produce more hydrophilic fibrous scaffolds with higher mass loss rates for cartilage tissue engineering application. First, the effects of diverse factors on the average and distribution of fiber's diameter of PHB scaffolds were systematically evaluated by experimental design. Then, PHB 9 wt % solutions were blended with various ratios of CTS (5%, 10%, 15%, and 20%) using trifluoroacetic acid as a co‐solvent, and electrospun. The addition of CTS could decrease both water droplet contact angle from ∼74° to ∼67° and tensile strength from, ∼87 MPa to ∼31 MPa. According to the results, the scaffolds containing 15% and 20% CTS were selected as optimized scaffolds for further investigations. Mass loss percentage of these scaffolds was directly proportional to the amount of CTS. Chondrocytes attached well to the surfaces of these scaffolds. The findings suggested that PHB/CTS blend fibrous scaffolds have tremendous potentials for further investigations for the intended application. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44171.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 77%

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Electrospun poly(hydroxybutyrate)/chitosan blend fibrous scaffolds for cartilage tissue engineering

Sadeghi

Karbasi

Razavi

et al. 2016

J of Applied Polymer Sci

113

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“…Pore size of 40-60 mm, suitable surface roughness and improved hydrophilicity of PHB/CHT scaffolds promoted fibroblasts cell proliferation. 51 Furthermore, optimum porosity (67%), controlled swelling and biodegradation (45% weight loss over 28 days) of PHBV/chitin led to enhanced human dermal fibroblasts cell attachment and proliferation compared to the control. 52 Similarly, electrospun PHBV/CHT (4:1 w/ w) with pore area 5.3 310 4 nm 2 and degradation rate of 26% exhibited higher levels of vinculin (an intracellular protein involved in focal adhesion) and wound healing potential compared to a 2/3 blend.…”

Section: Skin Tementioning

confidence: 92%

“…Cells formed clusters on P(3HB) and its composites, while they formed a confluent layer on P(4HB) and its composites (Figure ) due to the decrease in hydrophobicity of P(4HB) and its composites compared to P(3HB). Pore size of 40–60 µm, suitable surface roughness and improved hydrophilicity of PHB/CHT scaffolds promoted fibroblasts cell proliferation . Furthermore, optimum porosity (67%), controlled swelling and biodegradation (45% weight loss over 28 days) of PHBV/chitin led to enhanced human dermal fibroblasts cell attachment and proliferation compared to the control .…”

Section: Other Te Applications: Potential Of Phasmentioning

confidence: 94%

Third generation poly(hydroxyacid) composite scaffolds for tissue engineering

et al. 2016

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Bone tissue engineering based on scaffolds is quite a complex process as a whole gamut of criteria needs to be satisfied to promote cellular attachment, proliferation and differentiation: biocompatibility, right surface properties, adequate mechanical performance, controlled bioresorbability, osteoconductivity, angiogenic cues, and vascularization. Third generation scaffolds are more of composite types to maximize biological-mechanical-chemical properties. In the present review, our focus is on the performance of micro-organism-derived polyhydroxyalkanoates (PHAs)-polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-valerate (PHBV)-composite scaffolds with ceramics and natural polymers for tissue engineering applications with emphasis on bone tissue. We particularly emphasize on how material properties of the composites affect scaffold performance. PHA-based composites have demonstrated their biocompatibility with a range of tissues and their capacity to induce osteogenesis due to their piezoelectric properties. Electrospun PHB/PHBV fiber mesh in combination with human adipose tissue-derived stem cells (hASCs) were shown to improve vascularization in engineered bone tissues. For nerve and skin tissue engineering applications, natural polymers such as collagen and chitosan remain the gold standard but there is scope for development of scaffolds combining PHAs with other natural polymers which can address some of the limitations such as brittleness, lack of bioactivity and slow degradation rate presented by the latter. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1667-1684, 2017.

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Development of a membrane and a bilayer of chitosan, gelatin, and polyhydroxybutyrate to be used as wound dressing for the regeneration of rat excisional wounds

Barbosa,

de Melo,

da Silva Cunha

et al. 2023

J Biomedical Materials Res

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The skin is the largest organ in the human body that acts as a protective barrier from the outside environment. Certain dermatological pathologies or significant skin lesions can result in serious complications. Several studies have focused on the development of tissue‐engineered skin substitutes. In this study, a new bilayer scaffold composed of a chitosan‐gelatin membrane and a chitosan‐polyhydroxybutyrate (PHB) porous matrix was synthesized and populated with human adipose‐derived mesenchymal stem cells (hASCs) to be potentially used for wound dressing applications. By combining this membrane and porous matrix with the stem cells, we aimed to provide immunomodulation and differentiation capabilities for the wound environment, as well as mechanical strength and biocompatibility for the underlying tissue. The membrane was prepared from the mixture of chitosan and gelatin in a 2:1 ratio and the porous matrix was prepared from the mixture of chitosan and PHB, in equal proportions to form a final solution at 2.5% (m/v). Fourier transform infrared spectroscopy analysis showed the formation of blends, and micro‐computed tomography, scanning electron microscopy and atomic force microscopy images demonstrated membrane roughness and matrix porosity. The MTT assay showed that the scaffolds were biocompatible with hASC. The membrane and the bilayer were used as dressing and support for cell migration in the dorsal excisional wound model in Wistar rats. Histological and gene transcriptional analyses showed that the animals that received the scaffolds regenerated the hair follicles in the deep dermis in the central region of the wound. Our results demonstrate the potential of these new biomaterials as dressings in wound healing studies, favoring tissue regeneration.

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Properties and in vitro characterization of polyhydroxybutyrate–chitosan scaffolds prepared by modified precipitation method

Cited by 30 publications

References 52 publications

Electrospun poly(hydroxybutyrate)/chitosan blend fibrous scaffolds for cartilage tissue engineering

Electrospun poly(hydroxybutyrate)/chitosan blend fibrous scaffolds for cartilage tissue engineering

Third generation poly(hydroxyacid) composite scaffolds for tissue engineering

Development of a membrane and a bilayer of chitosan, gelatin, and polyhydroxybutyrate to be used as wound dressing for the regeneration of rat excisional wounds

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