Properties and biological functions of polysaccharides and ethanolic extracts isolated from medicinal fungus, Fomitopsis pinicola

Cheng, Jing-Jy; Lin, Cha-Yui; Lur, Huu‐Sheng; Chen, Hsuan-Pei; Lu, Mei-Kuang

doi:10.1016/j.procbio.2008.03.005

Cited by 49 publications

(27 citation statements)

References 39 publications

(44 reference statements)

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“…The combined extracts were lyophilized, and then 95% ethanol (10 ml) was added and polysaccharides were precipitated overnight at 4 ºC. The precipitated polysaccharides were collected after centrifugation (Centorion K24OR refrigerated centrifuge) at 3100 × g for 40 min followed by filtration, and then were lyophilized, resulting in a crude polysaccharidic sample (Cheng et al, 2008). The crude polysaccharidic samples were re-dissolved in water for the antioxidant activity assays, and further hydrolysed for chemical characterization.…”

Section: Preparation Of Polysaccharidic Extractsmentioning

confidence: 99%

“…The polysaccharidic extracts were hydrolyzed with 0.05 M trifluoroacetic acid (TFA, 2 ml), maintained at 90 ºC for 16h and then centrifuged, following a procedure described by Cheng et al (2008) with some modifications. The supernatant was lyophilized, redissolved in distilled water (1 ml) and filtered through 0.2 µm nylon filters for HPLC-RI analysis, using the conditions previously described by the authors (Grangeia, Heleno, Barros, Martins, & Ferreira, 2011).…”

Section: Characterization Of Polysaccharidic Extractsmentioning

confidence: 99%

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Fruiting body, spores and in vitro produced mycelium of Ganoderma lucidum from Northeast Portugal: A comparative study of the antioxidant potential of phenolic and polysaccharidic extracts

Heleno

Barros

Martins³

et al. 2012

Food Research International

135

107

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Abbreviations CATCatalase DAD Diode array detector DPPH 2,2-Diphenyl-1-picrylhydrazyl EC 50Extract concentration providing 50% antioxidant activity or 0.5 absorbance in the reducing power assay FB Fruiting body GAE Gallic acid equivalents GPx Glutathione peroxidase GSH Reduced glutathioneHPLC High-performance liquid chromatography IS Internal standard M Mycelium MMN Melin-Norkans medium MS Mass spectrometry PDA Potato dextrose agar medium PE Polysaccharides equivalents Ph Phenolic extract Ps Polysaccharidic extract RI Refraction index RSA Radical scavenging activity S Spores SOD Superoxide dismutaseTBARS Thiobarbituric acid reactive substances v/v ml/100 ml w/v g/100 ml ABSTRACTGanoderma lucidum is one of the most extensively studied mushrooms due to its medicinal properties. Herein, a systematic study was carried out in order to compare the antioxidant activity of phenolic and polysaccharidic extracts from fruiting body, spores and mycelium, obtained in three different culture media, of G. lucidum from NortheastPortugal. Phenolic extracts were characterized using high-performance liquid chromatography coupled to photodiode array detection, while polysaccharidic extracts were hydrolysed and further characterized using HPLC and refraction index detection.In general, the phenolic extracts (Ph) proved to have higher antioxidant potential than their corresponding polysaccharidic extracts (Ps). Amongst phenolic extracts, FB-Ph provided the highest antioxidant activity (EC 50 ≤ 0.6 mg/ml) and the highest content in total phenolics (~29 mg GAE/g extract) and phenolic acids (p-hydroxybenzoic and pcoumaric acids). S-Ps was the polysaccharidic extract with the best antioxidant activity (EC 50 ≤ 2 mg/ml); nevertheless, the highest levels of total phenolics were obtained in FB-PS (~56 mg GAE/g extract), while the highest levels of total polysaccharides (~14 mg PE/g extract) and individual sugars were observed in mycelia obtained from solid culture media, M-PDA-Ps and M-sMMN-Ps. The free radical scavenging properties, reducing power and lipid peroxidation inhibition of G. lucidum seemed to be correlated with phenolic compounds mostly in a free form, but also linked to polysaccharides.

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Section: Preparation Of Polysaccharidic Extractsmentioning

confidence: 99%

Section: Characterization Of Polysaccharidic Extractsmentioning

confidence: 99%

Fruiting body, spores and in vitro produced mycelium of Ganoderma lucidum from Northeast Portugal: A comparative study of the antioxidant potential of phenolic and polysaccharidic extracts

Heleno

Barros

Martins³

et al. 2012

Food Research International

135

107

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“…The IC50 values were calculated from the plots for each assay and are summarised in Table 1. In our earlier study, we had evaluated the antioxidant properties of edible mushrooms that were available in commercial Indian market (Rajesh Babu and Nageswar Rao 2011). Mushrooms are known to contain sterols (Ohnuma et al 2000), phenolic compounds and antioxidant vitamins like tocopherols and ascorbic acid, Josiana et al 2011), and polysaccharides (Cheng et al 2008). HPLC-MS analysis of edible mushrooms done by Barros et al (2009) revealed the presence of diverse phenolic acids like protocatechuic, p-hydroxybenzoic, p-coumaric and vanillic acid isomers.…”

Section: Electrochemical Behavior Of Mushroom Extractsmentioning

confidence: 99%

Antioxidant and electrochemical properties of cultivated Pleurotus spp. and their sporeless/low sporing mutants

2012

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Methanolic extracts of four cultivated edible mushrooms of Pleurotus spp. namely Pleurotus florida, Pleurotus sajor-caju, Pleurotus cystidiosus and Pleurotus djamor along with the sporeless/low sporing mutants of Pleurotus florida, and Pleurotus sajor-caju were analyzed for their antioxidant activity using different chemical assays. The electrochemical behaviors of these extracts were also analyzed using cyclic voltammetry and differential pulse voltammetry. Results showed that scavenging effects on 2,2-diphenyl-1-picrylhydrazyl radicals were good (73.3-42.4 %) at 1.5 mg/ml. At 12 mg/ml, the reducing powers (2.54-1.71) and chelating effects on ferrous ions (56.0-78.5 %) were excellent. H 2 O 2 scavenging abilities at 1.5 mg/ml showed a wide range (20.0-85.4 %). Scavenging of superoxide radicals were excellent and were found to be in the range of 61.1-90.0 % at 16 mg/ml concentration. FRAP results were in the range of 1.20 -0.98 at 16 mg/ ml. Total phenolic and total flavonoid contents of the methanolic extracts ranged from 22.67 to 36.03 mg/g and 1.19-2.94 μg/g respectively. The study assessed the amount of variation in antioxidant activities exhibited by different cultivated species and their sporeless/low sporing mutants.

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“…Medicinal fungus (Fomitopsis pinicola) is used extensively in the health sector due to its important chemical compounds (lanostane triterpenoids, triterpene glycosides and steroids) and antimicrobial, antitumor and antioxidant activities [1][2][3][4][5]. Additionally, it can be used as a nutritional supplement and as a therapeutic substance [5].…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Properties of Chitin and Chitosan Produced from Medicinal Fungus (Fomitopsis pinicola)

et al. 2014

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Fomitopsis pincola, which is used as a medicinal fungus in Asia, is widespread throughout the temperate Northern Hemisphere. The fungus's chitin structure was isolated and characterized in this study. It was found that 30.11 % of the dry weight of the fungus consisted of chitin, a very high portion. The chitosan yield from the chitin was 71.75 %. It was calculated that chitin acetylation was 72.5 % and that deacetylation of chitosan was 73.1 %. The maximum temperature of degradation (DTGmax) recorded for the chitin was 341°C and was 265°C for chitosan. The crystalline index (CrI) value of the chitin was 52 %, while it was 41 % for the chitosan. Examination by SEM revealed that the surface morphologies of the chitin and chitosan were formed of nanofibre structures. The FTIR examination identified it as the α form of chitin. As F. pinicola is widespread, abundant and has a high chitin and chitosan content, it may be used as an alternative chitin and chitosan source.

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Properties and biological functions of polysaccharides and ethanolic extracts isolated from medicinal fungus, Fomitopsis pinicola

Cited by 49 publications

References 39 publications

Fruiting body, spores and in vitro produced mycelium of Ganoderma lucidum from Northeast Portugal: A comparative study of the antioxidant potential of phenolic and polysaccharidic extracts

Fruiting body, spores and in vitro produced mycelium of Ganoderma lucidum from Northeast Portugal: A comparative study of the antioxidant potential of phenolic and polysaccharidic extracts

Antioxidant and electrochemical properties of cultivated Pleurotus spp. and their sporeless/low sporing mutants

Physicochemical Properties of Chitin and Chitosan Produced from Medicinal Fungus (Fomitopsis pinicola)

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