Properties and Biocompatibility of Chitosan and Silk Fibroin Blend Films for Application in Skin Tissue Engineering

Luangbudnark, Witoo; Viyoch, Jarupa; Laupattarakasem, Wiroon; Surakunprapha, Palakorn; Laupattarakasem, Pisamai

doi:10.1100/2012/697201

Cited by 84 publications

(64 citation statements)

References 44 publications

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“…The amide II band is divided into two peaks at 1514 and 1541 cm −1 confirming, therefore, that the composite contains both random coil and β sheet structures. The new peak appearing at 1260 cm −1 presented conformation transition from random coil to β-sheet of SF (Luangbudnark et al 2012). These results lead to the fact that in addition to the chitosan role in protecting silver nanoparticles from agglomeration by enveloping them, it can also increase the transition from random coil to beta sheet of SF.…”

Section: Discussionmentioning

confidence: 99%

Influence of the protocol of fibroin extraction on the antibiotic activities of the constructed composites

2015

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The effect of the solvents for silk fibroin (SF) extraction on its antimicrobial activity was studied. Extraction protocols were performed using LiBr (SFL) and Ajisawa’s reagent (CaCl2:ethanol:H2O) (SFC). The morphological and structural characteristics of the extracted SF and their composites were assessed. Corresponding bactericidal activities against Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922) and Pseudomonas aeroginosa (ATCC 27853) were performed. The resulting solutions were either casted into films or individually incorporated into composites of silver nanoparticles (NS) embedded into chitosan fragments (Cs) through γ-irradiation. Films of SF, obtained by using the two solvents, as well as the final prepared composites of SF, NS and Cs were analyzed using XRD, FTIR, SEM, TEM and zeta potential at several pH values. The band gap values were calculated. The results proved that, although SFC consumed shorter gelation time, yet SFL exerted higher antibiotic activity against the tested microorganisms. Moreover, the final composites had the ability to significantly reduce the growth of these medically relevant bacteria and are, therefore, recommended as a novel natural antibacterial biomaterial for several biomedical applications.

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Section: Discussionmentioning

confidence: 99%

Influence of the protocol of fibroin extraction on the antibiotic activities of the constructed composites

2015

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“…At 60% compressive strain level, all prepared scaffolds in this study showed >10 MPa strength with similar porosity (>72%). Pure SF, especially in dry state, lacks structural stability . PVA blending gives the desired mechanical integrity in these scaffolds to bear the load of human body.…”

Section: Discussionmentioning

confidence: 97%

Tissue engineering of human knee meniscus using functionalized and reinforced silk‐polyvinyl alcohol composite three‐dimensional scaffolds: Understanding the in vitro and in vivo behavior

Pillai

Janarthanan

Kumar

et al. 2018

J Biomedical Materials Res

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Tissue engineered constructs with rapid restoration of mechanical and biological properties remain a challenge, emphasizing the need to develop novel scaffolds. Here, we present a multicomponent composite three-dimensional scaffold structure with biomimetic reinforcement and biomolecule functionalization for meniscus tissue engineering. The scaffold structure was developed using 3:1 silk fibroin (SF) and polyvinyl alcohol (PVA). Autoclaved eggshell membrane (AESM) powder (1-3%w/v) was used as reinforcement to enhance biomechanical properties. Further to improve cell attachment and proliferation, these scaffolds were functionalized using an optimized unique combination of biomolecules. Comprehensive analysis of scaffolds was carried out on morphological, structural, mechanical and biological functionalities. Their mechanical properties were compared with different native human menisci. The results indicated that, functionalized SF-PVA with 3%AESM has shown similar order of magnitude of compressive and dynamic mechanical properties as in human meniscus. Moreover, 3% AESM based scaffolds were found to support better primary human meniscal cellular proliferation and extracellular matrix secretion. Immunohistochemical analysis revealed angiogenesis and biocompatibility with minimal inflammatory response for subcutaneously implanted scaffolds in New Zealand white rabbits. The developed reinforced and functionalized SF-PVA scaffolds can uniquely combine the potential for load-bearing properties with improved in vitro and in vivo support for meniscus tissue regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1722-1731, 2018.

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“…Higher absorption and permeability on body exudation could improve the healing of diabetic cutaneous wound. 29 Furthermore, chitosan has been described as being delivered in controlled protein release devices based on its cationic nature. An ionic complex can be formed between chitosan and growth factors, and these stable but reversible interactions can protect bioactive factors from infiltrating the surrounding environments.…”

Section: Discussionmentioning

confidence: 99%

Controlled Release of Thymosin Beta 4 Using a Collagen–Chitosan Sponge Scaffold Augments Cutaneous Wound Healing and Increases Angiogenesis in Diabetic Rats with Hindlimb Ischemia

Hao

Xia

et al. 2015

Tissue Engineering Part A

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It is important to establish an efficient vascularization for the long-term acceptance of bioengineered skin equivalents treating the cutaneous wounds of diabetic rats with hindlimb ischemia. This study investigates the possible use of a collagen-chitosan sponge scaffold encapsulated with thymosin beta 4 (CCSS-eTβ4), an angiogenic factor, to accelerate cutaneous wound healing in streptozotocin (STZ)-induced diabetic rats with hindlimb ischemia. CCSSs-eTβ4 was fabricated using a freeze-drying method. The scaffolds were analyzed by scanning electron microscopy, swelling and degradation assays, mechanical properties, and scaffolds of 50:50 collagen-chitosan were selected and applied. The controlled release of Tβ4 from the scaffolds elicited localized and prolonged effects over 12 days, as shown by an enzyme-linked immunosorbent assay (ELISA). In vivo, CCSSs-eTβ4 improved diabetic cutaneous wound healing, with faster wound reepithelialization, better dermal reorganization, and higher wound vascularization. Furthermore, CCSSs-eTβ4 downregulated inflammatory genes and upregulated angiogenic genes in the wound tissue. Significant increases in CD31-positive endothelial cells and new vessel density were also observed. In vitro, Tβ4 increased the migratory and proliferative activity of high glucose (HG)-treated human umbilical vein endothelial cells (HUVECs). Meanwhile, we found that Tβ4 could promote HG-treated HUVECs migration and improve angiogenesis by activation of the VEGF/AKT pathway. Overall, these findings demonstrated the promising potential of CCSSs-eTβ4 to promote more effective wound healing and suggest its possible application for diabetic cutaneous wound treatment.

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Properties and Biocompatibility of Chitosan and Silk Fibroin Blend Films for Application in Skin Tissue Engineering

Cited by 84 publications

References 44 publications

Influence of the protocol of fibroin extraction on the antibiotic activities of the constructed composites

Influence of the protocol of fibroin extraction on the antibiotic activities of the constructed composites

Tissue engineering of human knee meniscus using functionalized and reinforced silk‐polyvinyl alcohol composite three‐dimensional scaffolds: Understanding the in vitro and in vivo behavior

Controlled Release of Thymosin Beta 4 Using a Collagen–Chitosan Sponge Scaffold Augments Cutaneous Wound Healing and Increases Angiogenesis in Diabetic Rats with Hindlimb Ischemia

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