Finance Code 001. I also thank the partnership of LASURF with the R&D project of ANP No. 20358-8 (PUC-Rio/Shell Brasil/ANP), funded by Shell Brasil, for the support and resources provided.Family, friends, and lab colleagues who became friends.Finally, I would like to express my gratitude to myself, as even in the face of challenges posed by a pandemic that shook even the strongest of psyches and a government that discredited and undermined science in Brazil, I managed to complete this thesis.