Propagation Techniques for Annona Cherimola Mill.

Tazzari, L.; Pestelli, P.; Fiorino, P.; Parri, G.

doi:10.17660/actahortic.1990.275.38

Cited by 11 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other attempts to propagate in vitro the cherimoya failed due to permanent systemic contamination of the cultures (TAZZARI et al, 1990). After the first success with the micropropagation of cherimoya and after the studies of BEJOY and HARIHARAN (1992), BLAKE (1996a, 1996b) recorded similar results micropropagating another two important Annonaceae species: Annona muricata and Annona squamosa.…”

Section: Micropropagationmentioning

confidence: 84%

“…Some authors such as GEORGE and SHERRINGTON (1984), TAZZARI et al (1990) studying juvenile Annona cherimola and (JORDAN, (1988), JORDAN et al (1991), JORDAN and BOTTI (1992) studying the cultivar Concha Lisa reported very good response in the induction and development of adventitious shoots from hypocotyls, unfortunately with a low rate of rooting success. Working with the cultivar Fino de Jete it was obtained similar results enhancing the rates of adventitious shoot formation and achieving a high rooting percentage (near the 100%) (ENCINA et al, unpublished data).…”

Section: Adventitious Organogenesis Cellular Cultures and Regenerationmentioning

confidence: 99%

See 1 more Smart Citation

Biotechnology applied to Annona species: a review

et al. 2014

View full text Add to dashboard Cite

Annonaceae is an ancient family of plants including approximately 50 genera growing worldwide in a quite restricted area with specific agroclimatic requirements. Only few species of this family has been cultivated and exploited commercially and most of them belonging to the genus Annona such as A. muricata, A. squamosa, the hybrid A. cherimola x A. squamosa and specially Annona cherimola: the cherimoya, commercially cultivated in Spain, Chile, California, Florida, México, Australia, Ecuador, Peru, Brazil, New Zealand and several countries in South and Central America. The cherimoya shows a high degree of heterozygosis, and to obtain homogeneous and productive orchards it is necessary to avoid the propagation by seeds of this species. Additionally, the traditional methods of vegetative propagation were inefficient and inadequate, due to the low morphogenetic potential of this species, and the low rooting rate. The in vitro tissue culture methods of micropropagation can be applied successfully to cherimoya and other Annona sp to overcome these problems. Most of the protocols of micropropagation and regeneration were developed using the cultivar Fino de Jete, which is the major cultivar in Spain. First it is developed the method to micropropagate the juvenile material of cherimoya (ENCINA et al., 1994), and later it was optimized a protocol to micropropagate adult cherimoya genotypes selected by outstanding agronomical traits (PADILLA and ENCINA, 2004) and further it was improved the process through micrografting (PADILLA and ENCINA, 2011).At the present time we are involved in inducing and obtaining new elite genotypes, as part of a breeding program for the cherimoya and other Annonas, using and optimizing different methodologies in vitro: a) Adventitious organogenesis and regeneration from cellular cultures (ENCINA, 2004), b) Ploidy manipulation of the cherimoya, to obtain haploid, tetraploid and triploid plants (seedless), c) Genetic transformation, for the genes introduction to control the postharvest processes and the genes introduction to provide resistance to pathogen and insects and d) Micropropagation and regeneration of other wild Annona or related Annonaceae species such as: Annona senegalensis, A. scleroderma, A. montana, A. reticulata, A. glabra, A. diversifolia and Rollinia sp.

show abstract

Section: Micropropagationmentioning

confidence: 84%

Section: Adventitious Organogenesis Cellular Cultures and Regenerationmentioning

confidence: 99%

Biotechnology applied to Annona species: a review

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Micropropagation has been used in other species of Annona viz. Annona Cherimola [4,5] and Annona muricuta [6]. Surface sterilization is the first most important step in micropropagation.…”

Section: Introductionmentioning

confidence: 99%

Identification of Best Surface Sterilization Treatment and Control of Endophytic Bacterial Contamination in Annona squamosa L.

Minipara

Dhaduk

Patil

et al. 2019

IJPSS

View full text Add to dashboard Cite

Surface sterilization is most important step in plant tissue culture protocol. In the present investigation, an attempt was made to eliminate microbial and fungal contaminants from the surface and interior of plant material, thus obtaining axenic culture with highest survival rate. Sequential surface sterilizations of hypocotyl, leaf, shoot tip and mature node were carried out to investigate its effectiveness in controlling surface contamination with satisfactory survival of explants. Combination of different surfactant were used for surface sterilization treatments. The least contamination was obtained when hypocotyl explants were treated with 200 ppm cefotaxime and 500 ppm carbendazim along with 0.1% HgCl2 with best survival percentage. Treatments consisting of alcohol treatment, carbendazim (2000 ppm) followed by 1000 ppm cefotaxime, 500 ppm kanamycin, 2% sodium hypochloride and 0.1% HgCl2 sequentially resulted in complete elimination of surface contaminants from shoot tip, soft node and hard node obtained from field grown mature tree. Optimal elimination of bioburden from young leaf (77.38%) were obtained using 1000 ppm carbendazim, 500 ppm cefotaxime, 500 ppm kanamycin and 0.1% HgCl2. Gentamicin used in the medium was able to control the endophytic bacterial bioburden completely in the first cycle of 15 days itself at higher concentration of 96 mol/l to remove endophytic bacterial contamination with out effecting plant growth.

show abstract