Propagation physiology of Juniperus phoenicea L. from Jordan using seeds and in vitro culture techniques: Baseline information for a conservation perspective

Al-Ramamneh, Ezz Al-Dein; Dura, Susan; Daradkeh, Nidal Q.

doi:10.5897/ajb12.485

Cited by 4 publications

(2 citation statements)

References 41 publications

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“…The use of cold stratification gave contradictory results in previous studies. Pacini and Piotto [7] found that it was totally ineffective for J. macrocarpa, J. oxycedrus, and J. communis, whereas it increased the germination of J. phoenicea L. [34]. However, this pre-treatment was effective for the seed germination of other juniper species, such as J. excelsa from East Mediterranean and Caucasus areas [21], J. scopulorum Sarg.…”

Section: Discussionmentioning

confidence: 99%

Effects of pre-treatments and temperature on seed viability and germination of Juniperus macrocarpa Sm.

Pinna

Mattana

Cañadas

et al. 2014

Comptes Rendus. Biologies

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The effects of collecting season, collection site, laboratory pre-treatments and temperatures on seed viability and germination of Juniperus macrocarpa were investigated. Ripe cones were collected in four Sardinian dune systems, in two seasons, from plant and soil. Warm (W) and cold (C) stratifications, two combinations of them (W + C, C + W), and no pre-treatment (0) were applied. Seeds were incubated in a range of constant (10-25 8C) and an alternating (25/10 8C) temperature regime. Seed viability was low (ca. 40%) and varied significantly according to the collecting season. Seed germination was also low (ca. 10%), the 0 and W were the most effective pre-treatments on stimulating germination. The best germination temperature, without any pre-treatment, was 15 8C (ca. 20%). J. macrocarpa seeds are dormant and the achieved results suggested that the presence of secondary dormancy is induced by cold stratification. Spring appeared to be the best season for seed collecting, whereas autumn was the best for sowing. These results give new findings for restoration activities on this species.

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Section: Discussionmentioning

confidence: 99%

Effects of pre-treatments and temperature on seed viability and germination of Juniperus macrocarpa Sm.

Pinna

Mattana

Cañadas

et al. 2014

Comptes Rendus. Biologies

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“…On half-and quarter-strength WPM, shoot elongation was significantly reduced compared to the MS medium in Harpagophytum procumbens. Conversely, Fadel et al [48] observed the maximum shoot length on half-strength MS medium in Mentha spicata L.. Our results did not coincide with those of Loureiro et al [60], who observed that Juniperus phoenicea L. explants growing in DKW exhibited significantly better results compared to those growing in WPM or MS. Several studies in Juniperus species [61][62][63][64][65][66][67] have showed that optimal results in blastogenesis were achieved in media with a lower nitrogen content, i.e., diluted media. However, Bertsouklis et al [53] encountered challenges using the MS medium with low nitrogen content in an in vitro propagation of Juniperus phoenicea.…”

Section: In Vitro Culturementioning

confidence: 99%

Effects of Different Media and Their Strengths in In Vitro Culture of Three Different Cistus creticus L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular Markers

Ioannidis,

Koropouli

2024

Horticulturae

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Cistus creticus L. (rockrose), a species of ecological and medicinal significance, constitutes a valuable component of the Mediterranean ecosystem. The present study investigated the effect of the inorganic salt concentration of Murashige and Skoog medium (MS), woody plant medium (WPM), and Driver and Kuniyaki Walnut medium (DKW) at several strengths (1/8×, 1/4×, 1/2×, 1×, and 2×) on the in vitro growth and organogenesis of rockrose. Significant interactions were observed throughout the experiments between pairs of plant origins, medium types, and strengths, and we also examined the extent to which they affected the studied traits was examined. The types of nutrient medium affected all studied traits except shoot and root percentages. The maximum growth percentage (143.49%) was gained using full-strength WPM. The best performance in shoot percentage was obtained using MS (100%) at several strengths along with 1× WPM (100%). The topmost rooting percentage values (98.61%) were obtained using 1× WPM and 1/2× DKW. The highest number of shoots and roots were observed using full-strength MS (9.39) and half-strength WPM (6.49), respectively. The maximum values for shoot and root length were achieved using 1/2× MS (0.78 cm) and 1/8× WPM (1.55 cm), respectively. The origin of the plant material did not influence any studied trait. Moreover, the genetic relations among the populations used in the in vitro culture were assessed using simple sequence repeats (SSR) markers. Twenty-eight alleles were identified across all five STR loci. The different and effective alleles per locus were 5.60 and 4.72, respectively. The average observed and expected heterozygosity was estimated at 0.52 and 0.72, respectively. Shannon’s information index and the inbreeding coefficient (F) were assessed at 1.48 and 0.30, respectively, revealing a narrow genetic base and high genetic similarity among origins, suggesting that they belong to the same population.

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Effects of explant, media and growth regulators on in vitro regeneration and antioxidant activity of Juniperus phoenicea

Al-Ramamneh¹,

Daradkeh²,

Rababah³

et al. 2017

Aust J Crop Sci

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Juniperus phoenicea is an ornamental shrub that is also used to flavor food and to supply medicines and timber. Its micropropagation is of industrial concern and can occur by axillary shoot multiplication. Microcuttings of J. phoenicia were established in vitro on Murashige and Skoog (MS) and Rugini Olive (OM) media in glass tubes (25 mm x 150 mm). Factors studied were explant length (0.5 or 1.5 cm) and orientation (horizontal or vertical), media strength (OM, ½OM, MS, ½MS) and the following growth regulators: the anti-auxin 2,3,5-triiodobenzoic acid (TIBA), the cytokinins 6-benzyladenine (BA) and thidiazuron (TDZ), and the growth retardant daminozide (DM). Microcuttings placed vertically on the surface of OM, ½OM and ½MS media without hormones exhibited axillary bud differentiation, but they were swollen and turned brown one month later when placed horizontally on the medium surface. The number of shoots, averaged across OM, ½OM and ½MS media, was significantly higher from longer (1.5 cm) than shorter (0.5 cm) microcuttings (4.11 versus 1.57 shoots microcutting -1 ) after 60 days of culture. TIBA or DM at 0.1 mg l -1 included in OM medium enhanced leaf differentiation, callus induction and formation of adventitious shoots over three months from 0.5 cm long microcuttings taken from in vitro shoots. The formation of adventitious shoots was sporadic and occurred at a rate of 1 shoot microcutting -1 in the presence of 0.1 mg l -1 DM. OM supplemented with 0.1 mg l -1 TIBA resulted in significantly the highest leaf differentiation (55 leaves microcutting -1 ), with a rooting rate of 40%. Contents of phenols, flavonoids and antioxidants were compared for cuttings from young seedlings, callus, in vitro shoots, and seeds. Antioxidant activity was significantly the highest for shoots grown on hormone-free OM medium and callus maintained on OM medium containing 0.1 mg l -1 2,4-D (94.7 and 94.3% inhibition of DPPH free radicals, respectively). Thus, different routes for in vitro regeneration of J. phoenicea can be of potential use for many biotechnological, pharmaceutical and food industry applications.

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Propagation physiology of Juniperus phoenicea L. from Jordan using seeds and in vitro culture techniques: Baseline information for a conservation perspective

Cited by 4 publications

References 41 publications

Effects of pre-treatments and temperature on seed viability and germination of Juniperus macrocarpa Sm.

Effects of pre-treatments and temperature on seed viability and germination of Juniperus macrocarpa Sm.

Effects of Different Media and Their Strengths in In Vitro Culture of Three Different Cistus creticus L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular Markers

Effects of explant, media and growth regulators on in vitro regeneration and antioxidant activity of Juniperus phoenicea

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