PROPAGATION | Own-Rooted Cuttings

Costa, J.M.

doi:10.1016/b0-12-227620-5/00076-8

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Another support related to the higher rooting quality of 2020 cuttings is that these cuttings reached 100% rooting under 4,000 ppm IBA. Thus, it is argued that a less lignified and softer material can absorb and utilize external hormone better, thus enhancing the ease of rooting (Costa et al, 2017). Following the above observations, higher plant survival rates were also observed in 2020 under 2,000 ppm and 4,000 ppm IBA in apical cuttings.…”

mentioning

confidence: 61%

Asexual propagation for sustainable utilization of Greek indigenous germplasm of common dogwood (Cornus sanguinea L.), a neglected but noteworthy ornamental and nutraceutical species

Karapatzak¹,

Papanastasi²,

Kyrkas

et al. 2023

Not Bot Horti Agrobo

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The common dogwood (Cornus sanguinea L., Cornaceae) across the European continent represents a species with validated ornamental and nutraceutical value that has been utilized by humans for thousands of years but has been abandoned during modern times. It still represents however, a valuable but neglected native germplasm resource. The development of an asexual propagation protocol as a first step towards utilization of Greek C. sanguinea germplasm is presented herein for the first time. Plant material was collected from C. sanguinea‘s natural habitat during winter dormancy (hardwood cuttings) as well as during early vegetative growth (softwood cuttings). The propagation protocol was developed through preliminary trials for the successful propagation of the material sourced directly from the wild to establish enough starting material (ex situ adapted mother plants); consecutively, cutting propagation experiments over a two-year period coupled with early plant growth and survival assessment were conducted. The results showed that the use of external indole-3-butyric acid (IBA) rooting hormone application between 2,000 and 4,000 ppm delivered very high rooting rates (80-100%) via the use of primary softwood, leafy cuttings with minimum amount of lignification under mist. The proposed protocol is considered fast, reliable, easy to implement and economically viable. The study provides first time data on domestication and propagation of Greek C. sanguinea germplasm and at the same time paves the way for further research on the sustainable utilization of this species.

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confidence: 61%

Asexual propagation for sustainable utilization of Greek indigenous germplasm of common dogwood (Cornus sanguinea L.), a neglected but noteworthy ornamental and nutraceutical species

Karapatzak¹,

Papanastasi²,

Kyrkas

et al. 2023

Not Bot Horti Agrobo

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“…In general, auxins are the most commonly used phytohormones to promote adventitious root development [4,22,24,28,29], while cytokinins are used only to a very limited extent for rhizogenesis. Therefore, the effect of cytokinin on rhizogenesis in various species is still unknown.…”

Section: Discussionmentioning

confidence: 99%

Rapid and Efficient In Vitro Propagation Protocol of Endangered Wild Prickly Pear Growing in Eastern Morocco

et al. 2023

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The Opuntia genus is widely recognized as a significant member of the Cactaceae family. The eastern Moroccan prickly pear’s wild ecotype is renowned for its production of fruits of superior quality, which are in high demand. Nonetheless, the white cochineal (Dactylopius opuntiae) has emerged as a significant hazard to the persistence of the indigenous wild prickly pear population in the majority of the country’s territories. Conventional plant propagation techniques may facilitate the transmission of pathogens to successive generations and thus fail to satisfy market requirements. Therefore, the primary goal of this study was to develop a rapid and efficient protocol for large-scale production of the eastern Moroccan wild ecotype using axillary buds as the starting material. Sterilization of the starting material is a crucial but challenging step in this species, as the meristem is located just beneath the spine. The protocol developed for this study produced moderately satisfactory results, with 20 to 30% contamination in each experiment. The obtained shoots were incubated on Murashige and Skoog medium supplemented with varying concentrations of BAP (0, 1.5, 3, 5, and 7.5 mg/L). The treatment with BAP at 5 mg/L exhibited a statistically significant increase in the average number of regenerated shoots per explant (19.42). The effect of kinetin on the rooting of prickly pear was evaluated by transferring the shoots to a MS medium supplemented with varying concentrations of kinetin (0, 0.5, 1, 1.5, 2, and 2.5 mg/L). The use of kinetin increased the number and length of roots while also shortening the root development period from 21 days to 10 days. The best results were obtained at a concentration of 1.5 mg/L of kinetin. Furthermore, satisfactory acclimatization of plants was achieved by using plastic containers with a gradually increasing opening of the lids. The outcomes of this experimentation have significant potential to facilitate the preservation of this botanical variety, reduce the risk of white cochineal infestation, and address the need for superior quality fruit supply in the market.

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pH, dilución y concentraciones de alcohol de AIB en el enraizamiento de Ficus carica

Monroy González,

Villegas Monter,

Peña Valdivia

et al. 2024

Remexca

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La propagación vegetativa con estacas de tallo requiere originar brotes y raíces para formar una planta completa. Se han investigado factores que intervienen en la generación de raíces, pero poco se ha trabajado con el pH. La investigación se realizó en el Colegio de Postgraduados, Campus Montecillo, Texcoco, México en 2021. En estacas de Ficus carica se evaluaron: valores de pH (4, 7 y 11) en solución con o sin auxina, la dilución (alcohol o NaOH) del ácido indolbutírico (AIB), concentración de alcohol para disolver AIB (10, 15 y 20%), en el porcentaje de enraizamiento, número de raíces, longitud de la raíz más larga, porcentaje de yemas brotadas a los 21 días, fecha de establecimiento y cambios anatómicos en 0, 3 y 6 días de estacado. Se encontraron células meristemáticas y primordios de raíz hasta el día 6. Se obtuvo 100% de enraizamiento en todos los tratamientos. El pH no demostró tener efecto en la solución auxínica, pero si influyó en el porcentaje de enraizamiento evaluado individualmente. La forma de diluir la auxina influyó en el número de raíces por la concentración de alcohol utilizada, menor concentración (10%) tuvo efecto positivo.

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PROPAGATION | Own-Rooted Cuttings

Cited by 3 publications

References 6 publications

Asexual propagation for sustainable utilization of Greek indigenous germplasm of common dogwood (Cornus sanguinea L.), a neglected but noteworthy ornamental and nutraceutical species

Asexual propagation for sustainable utilization of Greek indigenous germplasm of common dogwood (Cornus sanguinea L.), a neglected but noteworthy ornamental and nutraceutical species

Rapid and Efficient In Vitro Propagation Protocol of Endangered Wild Prickly Pear Growing in Eastern Morocco

pH, dilución y concentraciones de alcohol de AIB en el enraizamiento de Ficus carica

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