Propagation of Trachoma Virus in Cultures of Human Tissues.

Pollard, Morris; Starr, Theodore J.; Tanami, Yoh; Moore, Richard W.

doi:10.3181/00379727-104-25786

Cited by 16 publications

(5 citation statements)

References 2 publications

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“…As reported by others (10,16) for trachoma strains, infectivity of strain B-577 for chicken embryos was maintained after becoming adapted to L cells.…”

Section: During Adaptation Of Chlamydial Strain B-577 To L Cells Censupporting

confidence: 52%

Tissue Culture Adaptation and Pathogenic Properties of an Ovine Chlamydial Abortion Strain*

Becerra

Storz

2010

Zentralblatt Für Veterinärmedizin Reihe B

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Summary The ovine chlamydial abortion strain B‐577 was adapted and maintained for 101 serial passages in cell cultures. Rates of chlamydial infection of cells ranged between 8.2 and 100%, and the infectivity in developing chicken embryos of the tissue culture‐adapted strain varied from 103.5 to 107.833 CELD50/0.5 ml. at different passage levels. Unexpected reductions of chlamydial infectivity occurred at the 55th, 56th and 93rd passages. The tissue culture‐adapted chlamydial strain B‐577 remained consistently pathogenic for chicken embryos, and caused placental and fetal infection after inoculation of pregnant ewes with material of the 54th passage in L cells. Furthermore, this strain caused an accidental laboratory infection of man after the 94th passage in tissue culture. These facts indicate that this chlamydial strain did not become attenuated during serial passage in culture cells. Zusammenfassung Pathogenität eines Chlamydienstammes vom Schafabort nach Adaption an Gewebekulturen Der Chlamydienstamm B 577 vom Schafabort wurde an L‐Zellen adaptiert und in 101 Passagen darin weitergezüchtet. Während dieser Passagen schwankte die Rate infizierter Zellen zwischen 8,2 und 100%. Der Infektionstiter in Hühnerembryonen lag zwischen 10−3,5 und 10−7,833 LD50. In der 55., 56. und 93. Gewebekulturpassage waren die Titer am niedrigsten. Die Pathogenität des Stammes für die Hühnerembryonen blieb unverändert. Nach der 54. L‐Zellen‐Passage infizierte dieser Stamm Plazenta und Fötus in tragenden Schafen. Außerdem verursachte der Stamm B 577 während der 94. Passage eine Augeninfektion beim Menschen. Diese Befunde weisen darauf hin, daß der Chlamydienstamm B 577 durch Passagen in L‐Zellen nicht attenuiert wurde. Résumé Pathogénité d'une souche de Chlamydia, isolée lors d'une avortement chez le mouton, après une adaptation aux cultures de tissue On a adapté aux cellules‐L la souche Chlamydia B 577 d'un avortement de mouton et on l'a reproduite lors de 101 passages. Le taux de cellules infectées alla de 8,2 à 100% durant ces passages. Le titre infectieux se situa chez l'embryon de poulet entre 10−3,5 et 10−7,833 LD50. Les titres furent les plus bas lors du 55e, 56e et 93e passage en culture de tissu. La pathogénité de la souche pour l'embryon de poulet resta inchangée. Cette souche infecta le placenta et le foetus de brebis gravides après le 54e passage sur cellules‐L. La souche B 577 provoqua en outre une infection oculaire chez l'homme pendant le 94e passage. Ces résultats montrent que la souche Chlamydia B 577 n'a pas été atténuée par des passages sur cellules‐L. Resumen Patogeneidad de una estirpe de clamidios del aborto ovino tras adaptación a cultivos hísticos Se adaptó la estirpe de clamidios B 577 del aborto ovino a células L y se siguió cultivando en ellas durante 101 pases. A lo largo de estos pases oscilaba la tasa de células infectadas entre 8,2 y 100%. El título de infección en los embriones de pollo se hallaba entre 10−3,5 y 10−7,833 DL50. Los títulos eran mínimos en los pases 55°, 56° y 93° por cultivo hístico. La...

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“…As reported by others (10,16) for trachoma strains, infectivity of strain B-577 for chicken embryos was maintained after becoming adapted to L cells.…”

Section: During Adaptation Of Chlamydial Strain B-577 To L Cells Censupporting

confidence: 52%

Tissue Culture Adaptation and Pathogenic Properties of an Ovine Chlamydial Abortion Strain*

Becerra

Storz

2010

Zentralblatt Für Veterinärmedizin Reihe B

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“…The propagation of chlamydiae in continuous cell culture, which was first introduced in the 1960s (Gordon et al, 1960;Pollard et al, 1960;Bernkopf et al, 1962;Gordon & Quan, 1965;Gordon et al, 1969) as an alternative to the more cumbersome cultivation in egg yolk sacs and primary/explant cultures, greatly facilitated isolation of chlamydiae for use in physiological studies. The introduction of isopycnic (density gradient) centrifugation as a means to obtain highly pure preparations of EBs and RBs moreover represented a key technological achievement that promoted characterization of the physiological features of distinct developmental forms (Tamura & Higashi, 1963;Allen, 1967;Tamura & Manire, 1967;Tamura et al, , 1971.…”

Section: The Discovery Of Developmental Stage-specific Metabolic Featmentioning

confidence: 99%

Chlamydial metabolism revisited: interspecies metabolic variability and developmental stage-specific physiologic activities

Omsland¹,

Sixt²,

et al. 2014

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Chlamydiae are a group of obligate intracellular bacteria comprising important human and animal pathogens as well as symbionts of ubiquitous protists. They are characterized by a developmental cycle including two main morphologically and physiologically distinct stages, the replicating reticulate body and the infectious non-dividing elementary body. In this review we reconstruct the history of studies that have led to our current perception of chlamydial physiology, focusing on their energy and central carbon metabolism. We then compare the metabolic capabilities of pathogenic and environmental chlamydiae highlighting interspecies variability among the metabolically more flexible environmental strains. We discuss recent findings suggesting that chlamydiae may not live as energy parasites throughout the developmental cycle and that elementary bodies are not metabolically inert but exhibit metabolic activity under appropriate axenic conditions. The observed host-free metabolic activity of elementary bodies may reflect adequate recapitulation of the intracellular environment, but there is evidence that this activity is biologically relevant and required for extracellular survival and maintenance of infectivity. The recent discoveries call for a reconsideration of chlamydial metabolism and future in-depth analyses to better understand how species- and stage-specific differences in chlamydial physiology may affect virulence, tissue tropism, and host adaptation.

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“…Early references to its use (18,20) indicate that it originated in C. M. Pomerat's laboratory. It has been used by a number of persons interested in Chlamydia (17), and cultures have frequently been exchanged. Although originally regarded as a human cell derived from synovial fluid, evidence has accumulated from several laboratories that cultures now labeled McCoy are composed of mouse cells.…”

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confidence: 99%

Effect of Ionizing Irradiation on Susceptibility of McCoy Cell Cultures to Chlamydia trachomatis

Gordon

Dressler

Quan

et al. 1972

Applied Microbiology

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The effect of. graded doses of irradiation (cobalt-60) on the morphology of McCoy cells was analyzed, and 4,000 to 5,000 r was selected as a satisfactory dose for production of giant cells. The susceptibility of radiation-induced giant cells to chlamydial infection was compared with that of nonirradiated cells by using three strains of Chlamydia trachomatis and one of C. psittaci. Monolayers of giant cells were more susceptible than normal McCoy cells as indicated by (i) greater numbers of inclusions (fourto eightfold) per unit area of monolayer, (ii) larger inclusions (fourfold greater in area), (iii) higher infective titers (1 log or more greater) of harvested cells, and (iv) greater ease of promoting a second cycle of growth. Graded doses of irradiation were applied also to mouse fibroblast (L) cells, and a similar increase in susceptibility to chlamydial infection was noted. It is concluded that giant cells produced by irradiation possess advantages over nonirradiated cells in culture for growth of Chlamydia.

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Propagation of Trachoma Virus in Cultures of Human Tissues.

Cited by 16 publications

References 2 publications

Tissue Culture Adaptation and Pathogenic Properties of an Ovine Chlamydial Abortion Strain*

Tissue Culture Adaptation and Pathogenic Properties of an Ovine Chlamydial Abortion Strain*

Chlamydial metabolism revisited: interspecies metabolic variability and developmental stage-specific physiologic activities

Effect of Ionizing Irradiation on Susceptibility of McCoy Cell Cultures to Chlamydia trachomatis

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