Propagation of Human Nasal Chondrocytes in Microcarrier Spinner Culture

Shikani, Alan H.; Fink, David J.; Sohrabi, Afshin; Phan, Phong V.; Polotsky, Anna; Hungerford, David S.; Frondoza, Carmelita G.

doi:10.1177/194589240401800207

Cited by 26 publications

(18 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found that chondrocytes attached to microcarriers can be cultured in a stirring bioreactor over more than 4 weeks with sustained high viability. While these results support previous studies, 6,9,19,20 we have shown for the first time that freshly isolated human articular chondrocytes readily attach to microcarriers derived from collagen type I without requiring intermediate monolayer isolation and expansion.…”

Section: Discussionsupporting

confidence: 56%

“…Faster cell doublings have also been reported for human nasal chondrocytes on porous microcarriers. 6,19,20 However, a direct comparison with our results is troublesome, since it has been demonstrated that monolayer-cultured nasoseptal cells proliferate 4 times faster than those of articular origin. 11 The higher population doubling times and increased microcarrier aggregation in microcarrier-cultured cells after 2-4 weeks observed in our studies indicate that cells reach confluence on the microcarrier surface, in contrast to TCP cultures where cells are reseeded at lower density during every passage.…”

mentioning

confidence: 64%

See 1 more Smart Citation

Adult human articular chondrocytes in a microcarrier‐based culture system: expansion and redifferentiation

Schrobback

Klein

Schuetz

et al. 2010

Journal Orthopaedic Research

View full text Add to dashboard Cite

Expanding human chondrocytes in vitro while maintaining their ability to form cartilage remains a key challenge in cartilage tissue engineering. One promising approach to address this is to use microcarriers as substrates for chondrocyte expansion. While microcarriers have shown beneficial effects for expansion of animal and ectopic human chondrocytes, their utility has not been determined for freshly isolated adult human articular chondrocytes. Thus, we investigated the proliferation and subsequent chondrogenic differentiation of these clinically relevant cells on porous gelatin microcarriers and compared them to those expanded using traditional monolayers. Chondrocytes attached to microcarriers within 2 days and remained viable over 4 weeks of culture in spinner flasks. Cells on microcarriers exhibited a spread morphology and initially proliferated faster than cells in monolayer culture, however, with prolonged expansion they were less proliferative. Cells expanded for 1 month and enzymatically released from microcarriers formed cartilaginous tissue in micromass pellet cultures, which was similar to tissue formed by monolayer-expanded cells. Cells left attached to microcarriers did not exhibit chondrogenic capacity. Culture conditions, such as microcarrier material, oxygen tension, and mechanical stimulation require further investigation to facilitate the efficient expansion of clinically relevant human articular chondrocytes that maintain chondrogenic potential for cartilage regeneration applications. ß

show abstract

Section: Discussionsupporting

confidence: 56%

mentioning

confidence: 64%

Adult human articular chondrocytes in a microcarrier‐based culture system: expansion and redifferentiation

Schrobback

Klein

Schuetz

et al. 2010

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…Research News particles into a defect, it overcomes the traditional surgical challenges with placement or suturing scaffolds and allows easier filling of the defect with the potential to improve surgical outcomes [63] . Furthermore, microparticle suspensions allow effective diffusion of nutrients and oxygen to cells and efficient removal of metabolic waste products.…”

Section: Advanced Materials Electrosprayed Injectable Microcapsules Amentioning

confidence: 99%

Living Cell Factories ‐ Electrosprayed Microcapsules and Microcarriers for Minimally Invasive Delivery

et al. 2015

View full text Add to dashboard Cite

Minimally invasive delivery of "living cell factories" consisting of cells and therapeutic agents has gained wide attention for next generation biomaterial device systems for multiple applications including musculoskeletal tissue regeneration, diabetes and cancer. Cellularbased microcapsules and microcarrier systems offer several attractive features for this particular purpose. One such technology capable of generating these types of systems is electrohydrodynamic (EHD) spraying. Depending on various parameters including applied voltage, biomaterial properties (viscosity, conductivity) and needle geometry, complex structures and arrangements can be fabricated for therapeutic strategies. In this paper, we outline the advances in the use of EHD technology specifically in the manipulation of bioactive and dynamic material systems to control size, composition and configuration in the development of minimally invasive micro-scaled biopolymeric systems. The exciting therapeutic applications of this technology, future perspectives and associated challenges are also presented.

show abstract

“…There are various designs ranging from the simplest stirred vessels to much more complex double chamber bioreactors for composite tissue formation. Regarding their application in cell culture, microcarriers are frequently used to increase the culture surface with the advantage of less enzymatic subcultivation steps, which turn out to be the main cause of cell dedifferentiation [34]. Recent studies have demonstrated that perfusion of a cell suspension directly through the pores of 3D scaffolds resulted in higher efficiencies and more uniform cell distributions inside the scaffold [35].…”

Section: Bioreactorsmentioning

confidence: 99%

Tissue-engineered trachea: History, problems and the future☆

Tan

Steiner

Hoerstrup

et al. 2006

European Journal of Cardio-Thoracic Surgery

View full text Add to dashboard Cite

SummaryThis review tries to summarize the efforts over the past 20 years to construct a tissue-engineered trachea. After illustrating the main technical bottlenecks faced nowadays, we discuss what might be the solutions to these bottlenecks. You may find out why the focus in this research field shifts dramatically from the construction of a tubular cartilage tissue to reepithelialization and revascularization of the prosthesis. In the end we propose a novel concept of 'in vivo bioreactor', defined as the design of a perfusion system inside the scaffold, and explain its potential application in the construction of a tissue-engineered trachea. # 2006 Published by Elsevier B.V.

show abstract

Propagation of Human Nasal Chondrocytes in Microcarrier Spinner Culture

Cited by 26 publications

References 26 publications

Adult human articular chondrocytes in a microcarrier‐based culture system: expansion and redifferentiation

Adult human articular chondrocytes in a microcarrier‐based culture system: expansion and redifferentiation

Living Cell Factories ‐ Electrosprayed Microcapsules and Microcarriers for Minimally Invasive Delivery

Tissue-engineered trachea: History, problems and the future☆

Contact Info

Product

Resources

About