Propagation of Duck Hepatitis Virus in Tissue Cultures Prepared With Collagenase

Abstract

Since the Smith and St. Johns-Brooks equation is predicated on a linear relation between log dose and log response, the evidence that a sigmoid relation obtains, at least subcutaneously, requires modification in the equation before further interpretations can be made of its parameters.

Collagenase in equine cell culture preparation

“…For longer periods at ambient temperature, Mandl (10) found better preservation in water than in phos-phate buffer. This observation, although irrelevant for the short digestion time used for cell dispersal, seems to have been the motive of some to chose GKN as solvent for the enzyme, a solution which contains NaCI, KCl, and glucose at concentrations of Hanks balanced salt solution (1,6,15). Collagenase in this solution may give acceptable digestion of tissues, but the diluent is not recommended, since it lacks calcium ions which are required for both the binding of the enzyme to the substrate and for full enzymatic effectiveness (10,11,19).…”

“…The tissue culture literature gives the collagenase concentrations usually in terms of the dry weight of collagenase per volume of solvent. Data vary from 0.4% (5, 14) to 2% (4, 7, 8), 0.01% (1,6,15) and 0.00025% (22).…”