1960
DOI: 10.1128/jb.80.4.571-572.1960
|View full text |Cite
|
Sign up to set email alerts
|

Propagation of Duck Hepatitis Virus in Tissue Cultures Prepared With Collagenase

Abstract: Since the Smith and St. Johns-Brooks equation is predicated on a linear relation between log dose and log response, the evidence that a sigmoid relation obtains, at least subcutaneously, requires modification in the equation before further interpretations can be made of its parameters.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

0
2
0

Year Published

1961
1961
2022
2022

Publication Types

Select...
5
1

Relationship

0
6

Authors

Journals

citations
Cited by 8 publications
(2 citation statements)
references
References 0 publications
0
2
0
Order By: Relevance
“…For longer periods at ambient temperature, Mandl (10) found better preservation in water than in phos-phate buffer. This observation, although irrelevant for the short digestion time used for cell dispersal, seems to have been the motive of some to chose GKN as solvent for the enzyme, a solution which contains NaCI, KCl, and glucose at concentrations of Hanks balanced salt solution (1,6,15). Collagenase in this solution may give acceptable digestion of tissues, but the diluent is not recommended, since it lacks calcium ions which are required for both the binding of the enzyme to the substrate and for full enzymatic effectiveness (10,11,19).…”
mentioning
confidence: 99%
See 1 more Smart Citation
“…For longer periods at ambient temperature, Mandl (10) found better preservation in water than in phos-phate buffer. This observation, although irrelevant for the short digestion time used for cell dispersal, seems to have been the motive of some to chose GKN as solvent for the enzyme, a solution which contains NaCI, KCl, and glucose at concentrations of Hanks balanced salt solution (1,6,15). Collagenase in this solution may give acceptable digestion of tissues, but the diluent is not recommended, since it lacks calcium ions which are required for both the binding of the enzyme to the substrate and for full enzymatic effectiveness (10,11,19).…”
mentioning
confidence: 99%
“…The tissue culture literature gives the collagenase concentrations usually in terms of the dry weight of collagenase per volume of solvent. Data vary from 0.4% (5, 14) to 2% (4, 7, 8), 0.01% (1,6,15) and 0.00025% (22).…”
mentioning
confidence: 99%