Propagation of a calcium pulse between osteoblastic cells

Xia, Shen‐Ling; Ferrier, Jack

doi:10.1016/s0006-291x(05)81535-9

Cited by 107 publications

(63 citation statements)

References 21 publications

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“…Indeed, stretch-activated ion channels have been identified on the plasma membrane of bone cells (41 ), and, more recently, propagation ofa Ca2" signal through osteoblastic monolayers has been demonstrated to occur after mechanical stimulation (42).…”

Section: Discussionmentioning

confidence: 99%

Connexin43 mediates direct intercellular communication in human osteoblastic cell networks.

Civitelli

Beyer²,

Warlow³

et al. 1993

J. Clin. Invest.

207

158

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Section: Discussionmentioning

confidence: 99%

Connexin43 mediates direct intercellular communication in human osteoblastic cell networks.

Civitelli

Beyer²,

Warlow³

et al. 1993

J. Clin. Invest.

207

158

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“…Besides, the anabolic response to PTH is attenuated in mice with an osteoblast-specific deletion of Cx43 [36]. Furthermore, it has been shown that calcium signals induced by direct membrane deformation are propagated via gap junctions to neighboring cells [37,38]. These data suggest that GJIC is critical to the mechanism by which external signals, both chemical (hormonal) and mechanical, can be transduced and integrated in bone cell networks.…”

Section: Introductionmentioning

confidence: 99%

Role of Cx43-Based Gap Junction in Murine Osteoblast-Like Mc3t3-E1Cells Exposed to 17-Βestradiol

Shang¹,

Wei²,

Liang³

2017

Mol Biol

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Estrogen has garnered considerable attention because of its importance in bone mass maintenance and the efficacy of hormone therapy in combating postmenopausal osteoporosis. Gap junctions are membrane spanning protein channels present on the surfaces of adjacent cells. They enable neighboring cells to physically link, which facilitate intercellular communication by allowing passage of small molecules from cell to cell in a process known as Gap Junctional Intercellular Communication (GJIC), can make the relevant cells function as a whole. An in vitro experiment combined with microarray analyses has identified novel genes in the response of MC3T3-E1 cells to an appropriate concentration of 17-β estradiol and the results of microarray showed that gap junction alpha-1 (Gja1) in the gap junction pathway was significantly elevated. We studied the effect of 17-β estradiol on the proliferation and differentiation of MC3T3-E1 cells disrupting of GJIC among osteoblasts with the chemical inhibitor, 18α-Glycyrrhetinic Acid (AGA). And we found that an appropriate concentration and duration of 17-β estradiol increased Methyl Thiazol Tetrazolium (MTT) values, Alkaline Phosphatase (ALP) activity and Runt-related transcription factor 2 (Runx2) proteins and gene expression and facilitated the mineralization of extracellular matrix. However, the promoting effect of 17-β estradiol on the proliferation and differentiation of MC3T3-E1 cells was weakened under the action of AGA. Therefore, we suggest that 17-β estradiol promotes MC3T3-E1's proliferation, differentiation and functions associated with Cx43-based GJIC, but gap junction is not the only signaling pathway that mediates the influence of 17-β estradiol on osteoblasts. The specific regulatory mechanism has yet to be researched.

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“…may pass~Flagg- Newton et al, 1979;Schwarzmann et al, 1981!. Cx43 is the predominant gap junction protein expressed by bone cells~Jones et al, 1993;Ilvesaro et al, 2000!. Gap junction channels allow intercellular transmission of signaling ions and second messenger molecules and have been thought to play several functions in bone, including the transmission of mechanical and chemical signals from one area of bone to another and the ability of the cellular network to initiate a coordinated response to external stimuli~Schiller et al, 1992;Xia & Ferrier, 1992;Donahue et al, 1995;Vander Molen et al, 1996;Jorgensen et al, 1997!. However, there is little direct evidence that osteocytes are functionally coupled via gap junctions.…”

Section: Introductionmentioning

confidence: 99%

Primary Cultures of Chick Osteocytes Retain Functional Gap Junctions between Osteocytes and between Osteocytes and Osteoblasts

Kamioka¹,

Ishihara²,

Ris³

et al. 2007

Microsc Microanal

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Abstract:The inaccessibility of osteocytes due to their embedment in the calcified bone matrix in vivo has precluded direct demonstration that osteocytes use gap junctions as a means of intercellular communication. In this article, we report successfully isolating primary cultures of osteocytes from chick calvaria, and, using anti-connexin 43 immunocytochemistry, demonstrate gap junction distribution to be comparable to that found in vivo. Next, we demonstrate the functionality of the gap junctions by~1! dye coupling studies that showed the spread of microinjected Lucifer Yellow from osteoblast to osteocyte and between adjacent osteocytes and 2! analysis of fluorescence replacement after photobleaching~FRAP!, in which photobleaching of cells loaded with a membrane-permeable dye resulted in rapid recovery of fluorescence into the photobleached osteocyte, within 5 min postbleaching. This FRAP effect did not occur when cells were treated with a gap junction blocker 18a-glycyrrhetinic acid!, but replacement of fluorescence into the photobleached cell resumed when it was removed. These studies demonstrate that gap junctions are responsible for intercellular communication between adjacent osteocytes and between osteoblasts and osteocytes. This role is consistent with the ability of osteocytes to respond to and transmit signals over long distances while embedded in a calcified matrix.

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Propagation of a calcium pulse between osteoblastic cells

Cited by 107 publications

References 21 publications

Connexin43 mediates direct intercellular communication in human osteoblastic cell networks.

Connexin43 mediates direct intercellular communication in human osteoblastic cell networks.

Role of Cx43-Based Gap Junction in Murine Osteoblast-Like Mc3t3-E1Cells Exposed to 17-Βestradiol

Primary Cultures of Chick Osteocytes Retain Functional Gap Junctions between Osteocytes and between Osteocytes and Osteoblasts

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