Propagation and corm development of Brodiaea in liquid cultures

Ilan, A.; Ziv, Meira; Halevy, A. H.

doi:10.1016/0304-4238(95)00785-r

Cited by 34 publications

(9 citation statements)

References 17 publications

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“…Chlorophyll content was also high in the leaves of plantlets grown under liquid cultures when compared to plantlets grown under semisolid cultures (Table 4). Ziv and Ariel (1991), Ilan et al (1995), andAdelberg (2004) reported similar effect of liquid cultures on increased adventitious bud differentiation accompanied biomass production in Philodendron, Brodiaea, and Hosta, respectively. PPFD affected biomass production and growth rate of Alocasia plantlets in liquid cultures (Table 5; Fig.…”

Section: Resultssupporting

confidence: 49%

“…Invariably, the propagules produced by bioreactor technology are hyperhydric; however, the propagation method developed in the present studies produce cormlets, which were effectively transplanted and acclimatized. Similarly, organogenic propagules (bulbs, tubers, or corms) have been successfully produced using in vitro cultures (Ziv and Ariel 1991;Ziv and Hadar 1991;Ilan et al 1995;Park et al 2000;Lian et al 2003;Kim et al 2004).…”

Section: Resultsmentioning

confidence: 97%

“…Recently, liquid cultures are efficiently used for the in vitro multiplication and rapid growth of the propagules (Ziv 1989;Ilan et al 1995;Park et al 2000;Lian et al 2003;Adelberg 2004;Kim et al 2004). After establishing the appropriate growth regulator and sucrose concentration for shoot proliferation, we have compared the semisolid cultures with liquid cultures for proliferation of shoots.…”

Section: Resultsmentioning

confidence: 99%

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Micropropagation of Alocasia amazonica using semisolid and liquid cultures

Murthy

Hahn

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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An efficient, simple micropropagation method was developed for Alocasia amazonica using corms in semisolid and liquid cultures. Explants were cultured onto Murashige and Skoog (MS) medium (Murashige and Skoog, Physiol. Plant. 15:473-497, 1962) supplemented with different cytokinins (Benzyladenine [BA, 2.22-13.32 μM], kinetin [2.32-13.95 μM], Thidiazuron [TDZ, 0.45-4.54 μM]) and cytokinin in combination with auxins [naphthalene acetic acid (NAA, 0.54-5.37 μM)/indole acetic acid (IAA, 0.57-5.71 μM)/indole butyric acid (IBA, 0.49-4.9 μM)]. All supplementary-induced shoot proliferation and the optimal results was on the medium supplemented with 2.27 μM TDZ, which induced 5.1 shoots per explant. Among the different concentrations of sucrose (0-120 g l −1 ) tested for shoot proliferation, 30 g l −1 was found suitable for corm cultures of Alocasia amazonica. The optimal shoot proliferation and biomass values were with the plantlets grown at 30 μmol m −2 s −1 photosynthetic photon flux (PPF) and 25°C. Liquid cultures found suitable for shoot proliferation and biomass accumulation was compared to semisolid cultures. Comparative studies of bioreactor systems [continuous immersion (with or without net) and temporary immersion in liquid media using ebb and flood] revealed that shoot multiplication and growth were greatest with the raft bioreactor system. Plantlets (cormlets) from the bioreactor were hydroponically cultured for 30 days, and 100% of plants were acclimatized successfully. The simple efficient method of production of plantlets (cormlets) is useful for large-scale multiplication of this important ornamental plant.

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Section: Resultssupporting

confidence: 49%

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

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Micropropagation of Alocasia amazonica using semisolid and liquid cultures

Murthy

Hahn

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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“…It is known that liquid culture, with or without cytokines, is generally more efficient than solid support systems for shoot proliferation and multiplication for several species of plants, including Allium species (Escalona et al, 1999;Ilan et al, 1995;Kim et al, 2003). To examine the effect of culture type and BA concentration on shoot multiplication, explants with shoots were divided into 2 to 4 segments and further subcultured in solid or liquid B5 medium supplemented with different concentrations of BA (0, 2.5, 5, or 10 μM) alone, or 10 μM BA in combination with 1 μM NAA.…”

Section: Effect Of Liquid Culture and Ba Concentration On Shoot Multimentioning

confidence: 99%

High-frequency Plant Regeneration from Cultured Flower Bud Receptacles of Allium hookeri L.

Koo¹

2014

HST

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Allium hookeri L. (Alliaceae family) is an important ethnomedicinal plant native to the Himalayan region of Asia. The aim of this research was to establish a high-frequency plant regeneration system for in vitro propagation of A. hookeri. Among the tissue types examined, receptacle explants derived from immature flower buds showed the highest regeneration rate of shoots (93.33 ± 4.63%), roots (76.67 ± 7.85%), and calli (80.00 ± 7.43%) when cultured on Gamborg B5 (B5) medium containing 10 μM 6-benzylaminopurine (BA) + 1 μM naphthalene acetic acid (NAA), 0.5 μM BA + 5 μM NAA, and 1 μM BA + 10 μM NAA, respectively. Shoot multiplication was superior when cultured in liquid rather than on solid medium and relatively high concentrations of BA, ranging from 5 to 10 μM. Efficient bulblet formation following root induction from shoot clumps was achieved with culture in liquid B5 medium containing 7% (w/v) sucrose. Regenerated bulblets were successfully acclimatized to ex vitro conditions with a greater than 95% survival rate. By this method, a maximum of 62 plantlets per receptacle could be propagated within 9 weeks of initial culture. The in vitro propagation system established in this study will promote A. hookeri biotechnology, including large-scale production of healthy and aseptic clones, preserving parental genotypes with desirable traits, and genetic manipulation to enhance medicinal value.

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“…Recently, liquid cultures are efficiently used for in vitro multiplication and rapid growth of the propagules (Ilan et al 1995;Park et al 2000;Adelberg 2004;Kim et al 2004;Jo et al 2008). After culturing leaf explants on semisolid medium for 4 weeks, explants were transferred to MS liquid medium (50 ml liquid medium taken in conical flask/s) with the same cytokinin concentrations as in semisolid cultures.…”

Section: Shoot Multiplication In Liquid Culturesmentioning

confidence: 99%

In vitro regeneration of brahmi shoots using semisolid and liquid cultures and quantitative analysis of bacoside A

et al. 2009

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The major objectives of this study were to investigate an efficient rapid protocol for mass propagation of adventitious shoots of brahmi using semisolid and liquid cultures; and to assess the amount of bacoside A accumulated in the regenerated shoots. Leaf explants were grown in vitro on Murashige and Skoog semisolid medium supplemented with 0.5, 1.0, 1.5, 2.0 and 2.5 mg l -1 6-benzyladenine or kinetin (KN) or thidiazuron (TDZ) for 4 weeks. Adventitious shoots developed from leaf explants on all cytokinin supplemented media. After 4 weeks of incubation, leaf explants were split into two batches and one set was subcultured on semisolid medium and another set in liquid medium containing same concentration of cytokinins where they have come from. Highest rate of shoot regeneration was observed for explants cultured on medium with 2 mg l -1 KN. The fresh and dry weight of shoots was also highest with this treatment. Liquid cultures were found suitable for proliferation of shoots (155.6 shoots per explant) and they also favored highest biomass accumulation (8.60 g fresh and 0.35 g dry biomass). The bacoside A contents were determined in shoots using HPLC. Analysis revealed that, the contents were highest with shoots regenerated on medium supplemented with 2 mg l -1 KN. The amount of bacoside A was highest in the shoots regenerated in liquid medium (11.92 mg g -1 DW) and it was 2.2-fold higher compared to shoots grown on semisolid cultures.

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Propagation and corm development of Brodiaea in liquid cultures

Cited by 34 publications

References 17 publications

Micropropagation of Alocasia amazonica using semisolid and liquid cultures

Micropropagation of Alocasia amazonica using semisolid and liquid cultures

High-frequency Plant Regeneration from Cultured Flower Bud Receptacles of Allium hookeri L.

In vitro regeneration of brahmi shoots using semisolid and liquid cultures and quantitative analysis of bacoside A

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