Proofreading Activity of <i>Pfu</i> Thermostable DNA Polymerase on a 6‐<i>O</i>‐Methylguanine‐Containing Template Monitored by ESI‐FTICR Mass Spectrometry

Wunschel, David S.; Masselon, Christophe; Feng, Bin; Smith, Richard D.

doi:10.1002/cbic.200400059

Cited by 3 publications

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References 21 publications

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“…For the unequivocal differentiation of the two overlapping isotopic distributions, all isotopic peaks must be resolved from one another regardless of their affiliation. We believe that not even the ultimate resolving power offered by FTICR mass spectrometers, which can achieve a value in excess of 1 000 000, would enable the separation of the practically congruent isotopic peaks within fused isotopic patterns of larger oligonucleotides (compare reference65 or the inset in Fig. 1 of66).…”

Section: Resultsmentioning

confidence: 99%

Characterization of synthetic nucleic acids by electrospray ionization quadrupole time‐of‐flight mass spectrometry

2005

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The potential of electrospray ionization quadrupole-quadrupole-time-of-flight mass spectrometry (ESI-QqTOF-MS) for the characterization of synthetic nucleic acids was evaluated. Oligonucleotides ranging in size from 12 up to 51 nucleotides were analyzed via direct infusion MS as well as via liquid chromatography (LC) online hyphenated to MS. These experiments proved the outstanding mass spectrometric performance of the TOF mass analyzer in regard of accuracy, reproducibility, resolution, and sensitivity. During a 1-min run, the monoisotopic mass of (dT)(24) was measured with a maximum relative mass deviation of 7.64 ppm proving the high mass accuracy of the TOF analyzer. Over a period of 1 h, mean deviations were determined in the range between -3.58 ppm and 3.06 ppm demonstrating the high stability of the applied external calibration. The molecular mass of a 51-mer was measured with a deviation smaller than 3.23 ppm from the theoretical value. The resolution exceeded a value of m/Deltam = 20 000 (m is the measured mass and Deltam the full peak width at half-maximum), which enabled the separation of the isotopic peaks of all investigated oligonucleotides. Because of the outstanding transmission and detection efficiency of the TOF mass analyzer, detection limits in the amol/microl to low fmol/microl range were reached. The usability of LC-ESI-QqTOF-MS for the qualitative and quantitative analysis of synthetic oligonucleotide mixtures was demonstrated.

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Section: Resultsmentioning

confidence: 99%

Characterization of synthetic nucleic acids by electrospray ionization quadrupole time‐of‐flight mass spectrometry

2005

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Current literature in mass spectrometry

2004

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Books, Reviews & Symposia; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (4 Weeks journals ‐ Search completed at 6th. Oct. 2004)

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Comprehensive Survey of Combinatorial Library Synthesis: 2004

Dolle

2005

J. Comb. Chem.

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An exhaustive compilation of biologically active libraries covering the years 1992-1997 was presented in a previous review [1a]. In that review, libraries were divided among 4 major categories, including those active against proteases, non-proteolytic enzymes, Gprotein coupled receptors, and non-G-protein coupled receptors. A generic structure for each library was provided as well as the structure of the most active member. The number of biologically active libraries reported in the literature during this time period (86 total) represents <25% of the total number of published library constructs. Disclosure of library synthesis without accompanying screening data is a more common occurrence. Because these types of libraries are equally important to the practicing combinatorial chemist, it was thought that a comprehensive listing of such libraries spanning the years 1992-1997 would be a useful supplement to the previous review [1a,b].Library constructs [2-248] listed herein are relegated to one of five tables. Table 1 is entitled 'Scaffold derivatization' and includes all constructs in which a multi-functional scaffold is modified in some fashion to create a library. An example of a construct found in Table 1 would be the sequential addition of three nucleophiles to trichloropyrimidine. Table 2, entitled 'Acyclic synthesis', lists all linear constructs such as those derived from multi-component Ugi condensations.

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Proofreading Activity of Pfu Thermostable DNA Polymerase on a 6‐O‐Methylguanine‐Containing Template Monitored by ESI‐FTICR Mass Spectrometry

Cited by 3 publications

References 21 publications

Characterization of synthetic nucleic acids by electrospray ionization quadrupole time‐of‐flight mass spectrometry

Characterization of synthetic nucleic acids by electrospray ionization quadrupole time‐of‐flight mass spectrometry

Current literature in mass spectrometry

Comprehensive Survey of Combinatorial Library Synthesis: 2004

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