Proof‐of‐concept study to establish an <i>in situ</i> method to determine the nature and depth of collagen changes in dentine using Fourier Transform Infra‐Red spectroscopy after sodium hypochlorite irrigation

Morgan, Alyn; Ng, YL; Odlyha, Marianne; Gulabivala, K; Bozec, Laurent

doi:10.1111/iej.13004

Cited by 16 publications

(15 citation statements)

References 44 publications

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“…For FTIR analysis, the protocol reported by Zhang et al (2010) and Morgan et al (2019) was followed. Briefly, dentine discs obtained from the middle third of the roots (n = 6) were embedded into acrylic resin, and dentine slices were obtained (1.5 mm thickness) using an Isomet saw (Buehler).…”

Section: Fourier Transform Infrared Spectroscopy (Ftir) Analysismentioning

confidence: 99%

Surface and structural changes in root dentine by various chelating solutions used in regenerative endodontics

et al. 2020

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Aim To evaluate the effects of the prolonged use of various irrigant solutions used during regenerative endodontic procedures on the physical and chemical structure of root canal dentine in extracted human teeth. Methodology Sixtyroot dentine samples from extracted, single‐rooted, human teeth were assigned to 10 groups. Eight groups were irrigated with 1.5% NaOCl for 5 min, followed by 3%, 10%, 17% EDTA or 10% citric acid (CA) for 5 or 10 min. One group received only NaOCl irrigation, and samples with only distilled water irrigation were used as a control group. The changes in microhardness and flexural strength were determined using Vickers and 3‐point flexural tester, respectively. Molecular and elemental compositions were recorded using FTIR and EDS spectroscopy. Data were analysed using one‐way anova, Kruskal–Wallis and Mann–Whitney U tests. Results The application of 1.5% NaOCl for 5 min did not affect the mineral content or microhardness of dentine (P > 0.05). However, it significantly decreased the dentine collagen peak values (P < 0.05), which was similar to the control group after the use of chelating agents (EDTA and 10% of CA) (P > 0.05). The effect of EDTA on the inorganic content was not concentration and time dependent (P > 0.05). CA resulted in a significantly greater reduction in the inorganic contents compared with the control and EDTA groups (P < 0.05). Regardless of the time, EDTA and CA significantly decreased dentine microhardness compared to the control (P < 0.05) with the greatest reduction in the CA groups (P < 0.05). NaOCl alone was associated with the lowest flexural strength, while none of the other irrigation regimens significantly decreased the flexural strength compared to the control group (P> 0.05). Conclusions Use of 1.5% NaOCl for 5 min decreased the collagen content of samples of human dentine from extracted teeth while EDTA and 10% citric acid mostly affected the inorganic content and microhardness of dentine surfaces. None of the irrigant solutions significantly decreased the mechanical properties of the entire dentine specimen.

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Section: Fourier Transform Infrared Spectroscopy (Ftir) Analysismentioning

confidence: 99%

Surface and structural changes in root dentine by various chelating solutions used in regenerative endodontics

et al. 2020

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“…Therefore, we envision the enzyme/antibiotics mixture being more effective when combined with mechanical instrumentation or other irrigants, such as NaOCl, at lower concentrations (below 2.25%). This lower concentration would decrease the erosion of the teeth and show reduced toxicity [ 42 , 43 , 44 , 45 ]. Moreover, a low concentration of NaOCl used against a mature E. faecalis biofilm in a root canal has been reported to significantly improve the disinfection when mechanical instrumentation or irrigation using Navitio FX was used [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Use of a Cellulase from Trichoderma reesei as an Adjuvant for Enterococcus faecalis Biofilm Disruption in Combination with Antibiotics as an Alternative Treatment in Secondary Endodontic Infection

et al. 2023

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Apical periodontitis is an inflammation leading to the injury and destruction of periradicular tissues. It is a sequence of events that starts from root canal infection, endodontic treatment, caries, or other dental interventions. Enterococcus faecalis is a ubiquitous oral pathogen that is challenging to eradicate because of biofilm formation during tooth infection. This study evaluated a hydrolase (CEL) from the fungus Trichoderma reesei combined with amoxicillin/clavulanic acid as a treatment against a clinical E. faecalis strain. Electron microscopy was used to visualize the structure modification of the extracellular polymeric substances. Biofilms were developed on human dental apices using standardized bioreactors to evaluate the antibiofilm activity of the treatment. Calcein and ethidium homodimer assays were used to evaluate the cytotoxic activity in human fibroblasts. In contrast, the human-derived monocytic cell line (THP-1) was used to evaluate the immunological response of CEL. In addition, the secretion of the pro-inflammatory cytokines IL-6 and TNF-α and the anti-inflammatory cytokine IL-10 were measured by ELISA. The results demonstrated that CEL did not induce the secretion of IL-6 and TNF-α when compared with lipopolysaccharide used as a positive control. Furthermore, the treatment combining CEL with amoxicillin/clavulanic acid showed excellent antibiofilm activity, with a 91.4% reduction in CFU on apical biofilms and a 97.6% reduction in the microcolonies. The results of this study could be used to develop a treatment to help eradicate persistent E. faecalis in apical periodontitis.

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“…NaOCl denatures or dissolves the collagen in dentine 103,[105][106][107][108][109] , leading to a reduction in elastic modulus, microhardness, flexural strength 100,110 and visco-elasticity, as well as an increase in strain upon loading of dentine or whole teeth 102,111 . Demineralising agents, such as EDTA or other acids, do not affect collagen, per se [103][104][105] but disrupt the inorganic matrix and expose the collagen fibrillar structure to further damage from NaOCl 103,108,109,112 .…”

Section: Effect Of Chemo-mechanical Root Canal Debridement On the Mechanical Properties Of Dentinementioning

confidence: 99%

Value of root-filled teeth in maintaining a functional dentition for life

Gulabivala

2019

Br Dent J

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Proof‐of‐concept study to establish an in situ method to determine the nature and depth of collagen changes in dentine using Fourier Transform Infra‐Red spectroscopy after sodium hypochlorite irrigation

Abstract: In extracted human teeth, NaOCl caused changes in dentinal collagen that were measurable by FTIR. In an ex vivo model, the depth of effect into dentine extended at least 0.5 mm from the canal wall.

Cited by 16 publications

References 44 publications

Surface and structural changes in root dentine by various chelating solutions used in regenerative endodontics

Surface and structural changes in root dentine by various chelating solutions used in regenerative endodontics

Use of a Cellulase from Trichoderma reesei as an Adjuvant for Enterococcus faecalis Biofilm Disruption in Combination with Antibiotics as an Alternative Treatment in Secondary Endodontic Infection

Value of root-filled teeth in maintaining a functional dentition for life

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