Proof of concept for inhibiting metastasis: circulating tumor cell-triggered localized release of anticancer agent via a structure-switching aptamer

Chen, Nandi; Yang, Xiaohai; Wang, Qing; Jian, Lixin; Shi, Hui; Qin, Shiya; Wang, Kemin; Huang, Jin; Liu, Wenjing

doi:10.1039/c6cc02374f

Cited by 19 publications

(12 citation statements)

References 22 publications

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“…It suggested that there is no loss happened during the assembly of four ssDNA, thus the concentration of DNA‐tetra was equal to the concentration of ssDNA. As shown in Figure 1B, the electrophoretic band of DNA‐1 containing one DNA aptamer sequence [ 23 ] was below 200 bp, and that of DNA‐3 containing three DNA aptamer sequences was above 200 bp. All the three DNA‐tetras ran slowly comparing with P2 (ssDNA, 106 bp), verifying the formation of DNA‐tetras.…”

Section: Resultsmentioning

confidence: 99%

Graphene Oxide Based Fluorescent DNA Aptasensor for Liver Cancer Diagnosis and Therapy

Liu

Wang

et al. 2021

Adv Funct Materials

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Graphene-oxide based fluorescent DNA aptasensors for the in vivo application remain a challenging task. We demonstrate for the first time such a nanomaterial for in vivo diagnosis and therapy of liver tumor with good biocompatibility and high selectivity. This DNA nanomaterial comprising of DNA tetrahedron and aptamers, aggregation-induced emission luminogen (AIEgens) and antitumor drug doxorubicin, is fabricated and attached on GO surface. Additionally, this GO-based fluorescent DNA nanodevice is also constructed by using microfluidic chip for liver tumor cell screening. File list (2) download file view on ChemRxiv Manuscript 20210308-Ke净版.docx (2.26 MiB) download file view on ChemRxiv Manuscript 20210308-Ke净版.pdf (1.27 MiB)

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Section: Resultsmentioning

confidence: 99%

Graphene Oxide Based Fluorescent DNA Aptasensor for Liver Cancer Diagnosis and Therapy

Liu

Wang

et al. 2021

Adv Funct Materials

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“…A versatile approach to aptamer immobilization involves the modification of SA or its analogues (avidin, neutravidin) on the surface and then further reacting with biotin-terminated aptamers. Initially, SA was physically adsorbed on the clean surface of a glass or PDMS substrate, ,− but with poor stability and controllability. Subsequently, covalent modification of SA was developed and attracted more research interest.…”

Section: Interface Functionalization Of Aptamersmentioning

confidence: 99%

Aptamer-Based Detection of Circulating Targets for Precision Medicine

et al. 2021

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The past decade has witnessed ongoing progress in precision medicine to improve human health. As an emerging diagnostic technique, liquid biopsy can provide realtime, comprehensive, dynamic physiological and pathological information in a noninvasive manner, opening a new window for precision medicine. Liquid biopsy depends on the sensitive and reliable detection of circulating targets (e.g., cells, extracellular vesicles, proteins, microRNAs) from body fluids, the performance of which is largely governed by recognition ligands. Aptamers are single-stranded functional oligonucleotides, capable of folding into unique tertiary structures to bind to their targets with superior specificity and affinity. Their mature evolution procedure, facile modification, and affinity regulation, as well as versatile structural design and engineering, make aptamers ideal recognition ligands for liquid biopsy. In this review, we present a broad overview of aptamer-based liquid biopsy techniques for precision medicine. We begin with recent advances in aptamer selection, followed by a summary of state-of-the-art strategies for multivalent aptamer assembly and aptamer interface modification. We will further describe aptamer-based micro-/nanoisolation platforms, aptamer-enabled release methods, and aptamer-assisted signal amplification and detection strategies. Finally, we present our perspectives regarding the opportunities and challenges of aptamer-based liquid biopsy for precision medicine.

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“…In light of the crucial roles of CTCs in metastasis, capturing and inhibiting CTCs would prevent metastasis. Wang’s group proposed CTC-triggered localized drug release strategies using structure-switching aptamers. , For example, they designed aptamer-functionalized DNA tetrahedrons containing chemotherapy drugs (doxorubicin, Dox) and a photosensitizer . As shown in Figure D, in the “sense” microchannel, the conformational change of aptamers upon the recognition of CTCs led to the release of DNA nanostructures, which subsequently flowed into “treat” microchannel.…”

Section: Aptamer-based Isolation Release and Analysis Of Ctcsmentioning

confidence: 99%

Aptamer-Based Liquid Biopsy

Wang

Zhu

et al. 2020

ACS Appl. Bio Mater.

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Liquid biopsy allows noninvasive and real-time analysis of tumor-derived circulating targets in body fluids, holding great value for cancer diagnosis, prognosis, personalized therapy, as well as the understanding of mechanisms underlying tumorigenesis and progression. However, the low concentration and heterogeneity of circulating targets and the complexity of body fluids bring daunting technical challenges to liquid biopsy. Aptamers with many unique properties are considered as promising recognition ligands for liquid biopsy. Their facile modification and affinity regulation afford numerous platforms for efficient isolation and release of circulating targets. With versatile structural design and engineering, aptamers also offer many strategies for sensitive detection and analysis of circulating targets. In this review, recent progress in aptamer-based liquid biopsy is summarized, with a focus on the isolation and detection of circulating tumor cells (CTCs) and extracellular vesicles (EVs). First, recently emerged strategies for evolving aptamers against circulating targets are introduced, and existing aptamers successfully applied in the liquid biopsy are comprehensively summarized, including their targets, sequences, affinities, etc. Then, aptamer-based approaches for CTC isolation, release, and analysis are summarized. Additionally, aptamer-mediated signal transduction and amplification for the detection of EVs are also reviewed. Finally, the challenges and future perspectives in this field are discussed.

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Proof of concept for inhibiting metastasis: circulating tumor cell-triggered localized release of anticancer agent via a structure-switching aptamer

Cited by 19 publications

References 22 publications

Graphene Oxide Based Fluorescent DNA Aptasensor for Liver Cancer Diagnosis and Therapy

Graphene Oxide Based Fluorescent DNA Aptasensor for Liver Cancer Diagnosis and Therapy

Aptamer-Based Detection of Circulating Targets for Precision Medicine

Aptamer-Based Liquid Biopsy

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