“…They have been widely used to infer activity patterns and they are normally scored visually, either as the presence of changes (AlOumaoui et al, 2004;Cardoso, 2008;Alves Cardoso and Henderson, 2010;Villotte et al, 2010) or on a scale of expression (Hawkey and Merbs, 1995;Chapman, 1997;Peterson, 1998;Steen and Lane, 1998;Weiss, 2003Weiss, , 2007Eshed et al, 2004;Jordana et al, 2006;Mariotti, et al, 2004Mariotti, et al, , 2007Molnar, 2006Molnar, , 2010Lieverse et al, 2008;Wysocki and Whittle, 2000;Henderson et al, 2012). Two-and threedimensional studies of ECs have also been undertaken (Wilczak, 1998;Zumwalt, 2005;Pany et al, 2009;Nolte and Wilczak, 2012).…”