Proneural genes in neocortical development

Wilkinson, Grey; Dennis, Daniel J.; Schuurmans, Carol

doi:10.1016/j.neuroscience.2013.08.029

Cited by 110 publications

(96 citation statements)

References 198 publications

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“…Importantly, ASCL1/hASH1 can determine different cell fates by promoting either proliferation or differentiation of neural progenitors (8,42). Thus, the local oxygen concentration may adjust ASCL1/hASH1 expression and associated pro-neural signaling pathways to the optimum level for the generation of different cell types in the developing central nervous system.…”

Section: Discussionmentioning

confidence: 99%

Shutdown of Achaete-scute Homolog-1 Expression by Heterogeneous Nuclear Ribonucleoprotein (hnRNP)-A2/B1 in Hypoxia

Kasim

Benko

Winkelmann

et al. 2014

Journal of Biological Chemistry

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Background:The transcription factor hASH1, encoded by the ASCL1 gene, has a crucial function in neurogenesis and tumor formation. Results: Gene expression of ASCL1 is controlled by the RNA-binding protein hnRNP-A2/B1 in neuroblastoma cells grown at low oxygen tension. Conclusion: ASCL1 mRNA is a new target of hnRNP-A2/B1. Significance: These findings provide novel insights in oxygen-dependent gene regulatory mechanisms in neuroblastoma cells.

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Section: Discussionmentioning

confidence: 99%

Shutdown of Achaete-scute Homolog-1 Expression by Heterogeneous Nuclear Ribonucleoprotein (hnRNP)-A2/B1 in Hypoxia

Kasim

Benko

Winkelmann

et al. 2014

Journal of Biological Chemistry

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“…Received May 27, 2016;revised version accepted August 3, 2016. During mammalian organogenesis, lineage specification and commitment involve passage through distinct progenitor/precursor states that rely on different combinations and levels of transcription factors (Wilkinson et al 2013;Cano et al 2014). In the current model of pancreatic endocrine cell formation, Neurogenin3 (Neurog3) expression in the epithelium rapidly progresses to a high-level production of protein (Neurog3 HI ) that leads to endocrine fate commitment, cell cycle exit, and delamination toward proto-islet clusters.…”

Section: Neurog3mentioning

confidence: 99%

Precommitment low-level Neurog3 expression defines a long-lived mitotic endocrine-biased progenitor pool that drives production of endocrine-committed cells

et al. 2016

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+ bipotent epithelial cells as the trigger for endocrine commitment, cell cycle exit, and rapid delamination toward proto-islet clusters. This model posits a transient Neurog3 expression state and short epithelial residence period. We show, however, that a Neurog3TA.LO cell population, defined as Neurog3 transcriptionally active and Sox9 + and often containing nonimmunodetectable Neurog3 protein, has a relatively high mitotic index and prolonged epithelial residency. We propose that this endocrine-biased mitotic progenitor state is functionally separated from a pro-ductal pool and endows them with long-term capacity to make endocrine fate-directed progeny. A novel BAC transgenic Neurog3 reporter detected two types of mitotic behavior in Sox9 + Neurog3TA.LO progenitors, associated with progenitor pool maintenance or derivation of endocrine-committed Neurog3 HI cells, respectively. Moreover, limiting Neurog3 expression dramatically increased the proportional representation of Sox9 + Neurog3 TA.LO progenitors, with a doubling of its mitotic index relative to normal Neurog3 expression, suggesting that low Neurog3 expression is a defining feature of this cycling endocrine-biased state. We propose that Sox9 + Neurog3 TA.LO endocrine-biased progenitors feed production of Neurog3 HI endocrine-committed cells during pancreas organogenesis.[Keywords: Neurog3; progenitor; endocrine-biased; mitotic] Supplemental material is available for this article. During mammalian organogenesis, lineage specification and commitment involve passage through distinct progenitor/precursor states that rely on different combinations and levels of transcription factors (Wilkinson et al. 2013;Cano et al. 2014). In the current model of pancreatic endocrine cell formation, Neurogenin3 (Neurog3) expression in the epithelium rapidly progresses to a high-level production of protein (Neurog3 HI ) that leads to endocrine fate commitment, cell cycle exit, and delamination toward proto-islet clusters. Meta-analysis of published literature (see below), however, is suggestive of a broader pattern of lower-level Neurog3 expression across the epithelium that is substantially more prevalent than the actively delaminating endocrine-committed Neurog3 HI population. The present study is focused on determining whether this low-Neurog3-expressing subpopulation represents the early-phase expression in post-mitotic cells on their way to becoming Neurog3 HI cells or endocrine fate-biased but uncommitted mitotic cells that have self-maintaining progenitor characteristics.Pancreas organogenesis is divided into a primary transition and secondary transition (Pan and Wright 2011). During the primary transition (embryonic day 9.5 [E9.5] to E12.5), multipotent progenitor cells undergo apical polarization, forming microlumens that then coalesce to generate an epithelial plexus. Within this epithelial plexus, progenitor cells (around E12.5) segregate into "tip" and "trunk" domains. In the short-term, tip domains

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“…The elevated levels of transforming growth factor, beta 1 (TGFB1) in ageing and neurodegenerative diseases have also been demonstrated (Kandasamy et al, 2014). The basic functions of the pro-neural gene, neurogenin 1 (NEUROG1), have been elucidated; it plays an important role in regulating the development of the olfactory system (Shaker et al, 2012), and in neocortical development (Wilkinson et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Effects of PAMAM dendrimers with various surface functional groups and multiple generations on cytotoxicity and neuronal differentiation using human neural progenitor cells

et al. 2016

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-Polyamidoamine (PAMAM) dendrimers have potential for biological applications as delivery systems for genes, drugs, and imaging agents into the brain, but their developmental neurotoxicity remains unknown. We investigated the effects of PAMAM dendrimers with various surface functional groups and multiple generations on neuronal differentiation using human neural progenitor cells at an equal mass concentration. Only PAMAM dendrimers containing amine (NH 2 ) surface groups at concentrations of 10 μg/mL significantly reduced cell viability and neuronal differentiation, compared with non-amine-terminated dendrimers. PAMAM-NH 2 with generation (G)3, G4, G5 G6, and G7 significantly decreased cell viability and inhibited neuronal differentiation from a concentration of 5 μg/mL, but G0, G1, and G2 dendrimers did not have any effect at this concentration. Cytotoxicity indices of PAMAM-NH 2 dendrimers at 10 μg/mL correlated well with the zeta potentials of the particles. Surface group density and particle number in unit volume is more important characteristic than particle size to influence cytotoxicity for positive changed dendrimers. PAMAM-50% C 12 at 1 μg/mL altered the expression level of the oxidative stress-related genes, ROR1, CYP26A1, and TGFB1, which is a DNA damage response gene. Our results indicate that PAMAM dendrimer exposure may have a surface charge-dependent adverse effect on neuronal differentiation, and that the effect may be associated with oxidative stress and DNA damage during development of neural cells.

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Proneural genes in neocortical development

Cited by 110 publications

References 198 publications

Shutdown of Achaete-scute Homolog-1 Expression by Heterogeneous Nuclear Ribonucleoprotein (hnRNP)-A2/B1 in Hypoxia

Shutdown of Achaete-scute Homolog-1 Expression by Heterogeneous Nuclear Ribonucleoprotein (hnRNP)-A2/B1 in Hypoxia

Precommitment low-level Neurog3 expression defines a long-lived mitotic endocrine-biased progenitor pool that drives production of endocrine-committed cells

Effects of PAMAM dendrimers with various surface functional groups and multiple generations on cytotoxicity and neuronal differentiation using human neural progenitor cells

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