Promotion of the growth and plant biomass degrading enzymes production in solid-state cultures of Lentinula edodes expressing Vitreoscilla hemoglobin gene

Wang, Xiaoting; Ding, Yatong; Gao, Xiaodan; Liu, Haohao; Gao, Yuqian; Qiu, Liyou

doi:10.1016/j.jbiotec.2019.06.301

Cited by 8 publications

(5 citation statements)

References 30 publications

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“…Vgb+ recombinant strain showed a more than about 7-fold higher vitamin A production (about 0.07 µg/ml) compared to its wild-type strain at 48 h. Results revealed that under low aeration conditions vitamin A production increased in the vgb+ strain. These results concur with the role for vgb gene done by other scientists (Kurt, Aytan, Ozer, Ates & Geckil, 2009;Mejía, Viniegra-González & Barrios-González, 2003;Du, Shen, Huang, Huang & Zhang, 2016;Wang et al, 2019).…”

Section: The Vhb Gene Leads To İncreased Production Of Vitamin a In ...supporting

confidence: 92%

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Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis

Giray¹,

Pekdemir

2022

Turkish JAF Sci.Tech.

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Vitamin A is an essential micronutrient and has important functions such as vision, growth, reproduction embryogenesis, cellular differentiation, and proliferation, immune function and epithelial protector in the organism. Biotechnological production of vitamins is increasing due to their advantages and significant advances. The vitreoscilla hemoglobin (VHb) gene is extremely effective in binding oxygen and conducting it under hypoxic conditions. In this study, the production of vitamin A in E. herbicola (wild type) and its recombinant strains was investigated in LB medium and M9 medium (containing high concentrations (1%) of different carbon sources). The maximum production of vitamin A of the recombinant strain with the hemoglobin gene (vgb+) was observed in including glucose and sucrose M9 medium and their total product levels in vgb+ recombinant strain were 0.14 µg/ml and 0.1 µg/ml, respectively. The vitamin A production in the M9 medium with glucose and sucrose were 2-fold and 1.4- fold higher than that of the wild strain, respectively. The extracellular product level (0.07 µg/ml) in LB was 7-fold higher than wild strain at 48 h. These results reveal that the expression of VHb in E. herbicola in the both LB and M9 medium (containing 1% glucose and 1% sucrose, specially) increase the vitamin A production.

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Section: The Vhb Gene Leads To İncreased Production Of Vitamin a In ...supporting

confidence: 92%

“…Production of many metabolites are increased by the expression of VHb (Gao, Deng, Guan, Liao & Cai, 2018;Giray, 2020;Kahraman, Aytan, Giray & Özcan, 2013;Kurt, Aytan, Ozer, Ates & Geckil, 2009;Li et al, 2016b;S. Wang et al, 2020;X. Wang et al, 2019).…”

Section: The Vitreoscilla Hemoglobin (Vhb) Did Not Induce Vitamin a P...mentioning

confidence: 99%

Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis

Giray¹,

Pekdemir

2022

Turkish JAF Sci.Tech.

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“…The pBHG-BCA1-gpd plasmid was kindly gifted to us by Professor Baogui Xie from the Fujian Agriculture and Forestry University. The resistance marker hygromycin B phosphotransferase (hph) gene resided in the plasmid [ 43 ]. E. coli BL21 and plasmid pCold-TF were purchased from Zomanbio (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

The Biosynthesis of 1-octene-3-ol by a Multifunctional Fatty Acid Dioxygenase and Hydroperoxide Lyase in Agaricus bisporus

Chen

Liu

et al. 2022

JoF

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The biosynthetic pathway from linoleic acid to 1-octen-3-ol in Agaricus bisporus has long been established, in which linoleic acid is converted to 10-hydroperoxide (10-HPOD) by deoxygenation, and 10-HPOD is subsequently cleaved to yield 1-octene-3-ol and 10-oxodecanoic acid. However, the corresponding enzymes have not been identified and cloned. In the present study, four putative genes involved in oxylipid biosynthesis, including one lipoxygenase gene named AbLOX, two linoleate diol synthase genes named AbLDS1 and AbLDS2, and one hydroperoxide lyase gene named AbHPL were retrieved from the A. bisporus genome by a homology search and cloned and expressed prokaryotically. AbLOX, AbLDS1, and AbLDS2 all exhibited fatty acid dioxygenase activity, catalyzing the conversion of linoleic acid to generate hydroperoxide, and AbHPL showed a cleaving hydroperoxide activity, as was determined by the KI-starch method. AbLOX and AbHPL catalyzed linoleic acid to 1-octen-3-ol with an optimum temperature of 35 °C and an optimum pH of 7.2, whereas AbLDS1, AbLDS2, and AbHPL catalyzed linoleic acid without 1-octen-3-ol. Reduced AbLOX expression in antisense AbLOX transformants was correlated with a decrease in the yield of 1-octen-3-ol. AbLOX and AbHPL were highly homologous to the sesquiterpene synthase Cop4 of Coprinus cinerea and the yeast sterol C-22 desaturase, respectively. These results reveal that the enzymes for the oxidative cleavage of linoleic acid to synthesize 1-octen-3-ol in A. bisporus are the multifunctional fatty acid dioxygenase AbLOX and hydroperoxide lyase AbHPL.

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“…[68], and ganoderic acid (1.4-fold) in G. lucidum [69]. For polysaccharides, several complex compounds, including bacterial cellulose (58.6%) in Gluconacetobacter xylinus [70], pullulan (42.08%) in Aureobasidium melanogenum [71], β-glucan (12.9-24.0%) in Lentinula edodes [72], and 6-(N-hydroxyethyl)-amino-6-deoxy-alpha-l-sorbofuranose (11.89%) in Gluconobacter oxydans [10] can be efficiently obtained, respectively. Enterobacter aerogenes [59] Enhancement: the ratio of the increase in the product titer of vgb + strain relative to the control (% or fold).…”

Section: Vhb In Biotechnological Productionsmentioning

confidence: 99%

Recent Advances in the Physicochemical Properties and Biotechnological Application of Vitreoscilla Hemoglobin

Zhao

Wang

et al. 2021

Microorganisms

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Vitreoscilla hemoglobin (VHb), the first discovered bacterial hemoglobin, is a soluble heme-binding protein with a faster rate of oxygen dissociation. Since it can enhance cell growth, product synthesis and stress tolerance, VHb has been widely applied in the field of metabolic engineering for microorganisms, plants, and animals. Especially under oxygen-limited conditions, VHb can interact with terminal oxidase to deliver enough oxygen to achieve high-cell-density fermentation. In recent years, with the development of bioinformatics and synthetic biology, several novel physicochemical properties and metabolic regulatory effects of VHb have been discovered and numerous strategies have been utilized to enhance the expression level of VHb in various hosts, which greatly promotes its applications in biotechnology. Thus, in this review, the new information regarding structure, function and expressional tactics for VHb is summarized to understand its latest applications and pave a new way for the future improvement of biosynthesis for other products.

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Promotion of the growth and plant biomass degrading enzymes production in solid-state cultures of Lentinula edodes expressing Vitreoscilla hemoglobin gene

Cited by 8 publications

References 30 publications

Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis

Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis

The Biosynthesis of 1-octene-3-ol by a Multifunctional Fatty Acid Dioxygenase and Hydroperoxide Lyase in Agaricus bisporus

Recent Advances in the Physicochemical Properties and Biotechnological Application of Vitreoscilla Hemoglobin

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